Identification and dipeptidyl peptidase IV (DPP-IV) inhibitory activity verification of peptides from mouse lymphocytes
The objective of this study was to isolate and identify the intracellular bioactive peptides from mouse lymphocytes before and after lipopolysaccharide (LPS) stimulation, to explore novel peptides and to research the bioactive function. Mouse spleen lymphocytes were isolated and cultured with LPS st...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2022-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022000921 |
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| author | Juan Wang Yujia Xie Yuanyuan Luan Tingting Guo Shanshan Xiao Xingxing Zeng Shaohui Zhang |
| author_facet | Juan Wang Yujia Xie Yuanyuan Luan Tingting Guo Shanshan Xiao Xingxing Zeng Shaohui Zhang |
| author_sort | Juan Wang |
| collection | DOAJ |
| description | The objective of this study was to isolate and identify the intracellular bioactive peptides from mouse lymphocytes before and after lipopolysaccharide (LPS) stimulation, to explore novel peptides and to research the bioactive function. Mouse spleen lymphocytes were isolated and cultured with LPS stimulation (experimental group) or not (control group) to collect intracellular peptides. Totally 385 peptides were analyzed by nanoliter liquid phase-Q Exactive quadrupole ultra-high resolution orbitrap mass spectrometer (Nano LC-Q Exactive Plus) and identified by PEAKS X software. After compared with peptides reported, 131 novel peptides were discovered, which then were predicted bioactivity by Peptide Ranker and 6 peptides with high bioactivity were predicted function by BIOPEP-UMW database. Prediction data showed that they may have dipeptidyl peptidase IV (DPP-IV) inhibitory activity. Finally, two peptides showed better potent inhibition were verified with competitive and noncompetitive modes. |
| format | Article |
| id | doaj-art-f1a08fc7860b4059aaa9c50772a82bed |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-f1a08fc7860b4059aaa9c50772a82bed2025-02-03T03:59:54ZengTsinghua University PressFood Science and Human Wellness2213-45302022-11-0111615151526Identification and dipeptidyl peptidase IV (DPP-IV) inhibitory activity verification of peptides from mouse lymphocytesJuan Wang0Yujia Xie1Yuanyuan Luan2Tingting Guo3Shanshan Xiao4Xingxing Zeng5Shaohui Zhang6School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Zhejiang Go Peptides Life Science and Healthcare Technology Co., Ltd., Wenzhou 325000, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaZhejiang Go Peptides Life Science and Healthcare Technology Co., Ltd., Wenzhou 325000, ChinaZhejiang Go Peptides Life Science and Healthcare Technology Co., Ltd., Wenzhou 325000, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Zhejiang Go Peptides Life Science and Healthcare Technology Co., Ltd., Wenzhou 325000, China; Corresponding author at: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.The objective of this study was to isolate and identify the intracellular bioactive peptides from mouse lymphocytes before and after lipopolysaccharide (LPS) stimulation, to explore novel peptides and to research the bioactive function. Mouse spleen lymphocytes were isolated and cultured with LPS stimulation (experimental group) or not (control group) to collect intracellular peptides. Totally 385 peptides were analyzed by nanoliter liquid phase-Q Exactive quadrupole ultra-high resolution orbitrap mass spectrometer (Nano LC-Q Exactive Plus) and identified by PEAKS X software. After compared with peptides reported, 131 novel peptides were discovered, which then were predicted bioactivity by Peptide Ranker and 6 peptides with high bioactivity were predicted function by BIOPEP-UMW database. Prediction data showed that they may have dipeptidyl peptidase IV (DPP-IV) inhibitory activity. Finally, two peptides showed better potent inhibition were verified with competitive and noncompetitive modes.http://www.sciencedirect.com/science/article/pii/S2213453022000921LymphocytesPeptidesLipopolysaccharideDipeptidyl peptidase IV (DPP-IV) inhibitory activity |
| spellingShingle | Juan Wang Yujia Xie Yuanyuan Luan Tingting Guo Shanshan Xiao Xingxing Zeng Shaohui Zhang Identification and dipeptidyl peptidase IV (DPP-IV) inhibitory activity verification of peptides from mouse lymphocytes Food Science and Human Wellness Lymphocytes Peptides Lipopolysaccharide Dipeptidyl peptidase IV (DPP-IV) inhibitory activity |
| title | Identification and dipeptidyl peptidase IV (DPP-IV) inhibitory activity verification of peptides from mouse lymphocytes |
| title_full | Identification and dipeptidyl peptidase IV (DPP-IV) inhibitory activity verification of peptides from mouse lymphocytes |
| title_fullStr | Identification and dipeptidyl peptidase IV (DPP-IV) inhibitory activity verification of peptides from mouse lymphocytes |
| title_full_unstemmed | Identification and dipeptidyl peptidase IV (DPP-IV) inhibitory activity verification of peptides from mouse lymphocytes |
| title_short | Identification and dipeptidyl peptidase IV (DPP-IV) inhibitory activity verification of peptides from mouse lymphocytes |
| title_sort | identification and dipeptidyl peptidase iv dpp iv inhibitory activity verification of peptides from mouse lymphocytes |
| topic | Lymphocytes Peptides Lipopolysaccharide Dipeptidyl peptidase IV (DPP-IV) inhibitory activity |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022000921 |
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