Effects of hypoxia on the heart of the juvenile four-finger threadfin (Eleutheronema tetradactylum) based on physiological indicators and transcriptome analysis
This study evaluated the effects of hypoxia on the heart of juvenile four-finger threadfin (Eleutheronema tetradactylum) through physiological and transcriptome analysis. Juveniles with an average weight of 122.82 g and length of 24.60 cm were used. Hypoxia significantly increased serum myocardial e...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2025.1530224/full |
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| author | Yi Lu Eric Amenyogbe Ye- Yang Zhong-liang Wang Zhong-liang Wang Zhong-liang Wang Jing-hui Jin Rui-tao Xie Eric Kwabena Droepenu Jian-sheng Huang Jian-sheng Huang Jian-sheng Huang |
| author_facet | Yi Lu Eric Amenyogbe Ye- Yang Zhong-liang Wang Zhong-liang Wang Zhong-liang Wang Jing-hui Jin Rui-tao Xie Eric Kwabena Droepenu Jian-sheng Huang Jian-sheng Huang Jian-sheng Huang |
| author_sort | Yi Lu |
| collection | DOAJ |
| description | This study evaluated the effects of hypoxia on the heart of juvenile four-finger threadfin (Eleutheronema tetradactylum) through physiological and transcriptome analysis. Juveniles with an average weight of 122.82 g and length of 24.60 cm were used. Hypoxia significantly increased serum myocardial enzyme activities, including creatine kinase (CK), creatine kinase-MB isoenzyme, lactate dehydrogenase (LDH), and α-hydroxybutyrate dehydrogenase (HDBH). These indicators initially rose and then declined, reflecting cardiac stress and suggesting their potential as early hypoxia biomarkers for real-time aquaculture monitoring. Histological analysis revealed structural damage in myocardial fibers under hypoxia, with increasing severity over time. This underscores the need to minimize oxygen fluctuations to prevent cardiac tissue degeneration. Transcriptome analysis identified upregulated genes involved in cell communication, immune responses, and intracellular signaling, offering potential targets for breeding hypoxia-tolerant species. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis highlighted key pathways such as mitogen-activated protein kinase (MAPK), hypoxia-inducible factor-1 (HIF-1), endocytosis, and phagosome formation. The MAPK pathway plays a critical role in cellular stress responses, including survival, proliferation, and apoptosis. Hypoxia-induced activation of MAPKs like ERK, JNK, and p38 regulates stress-responsive genes. HIF-1 signaling regulates oxygen homeostasis, with HIF-1α stabilizing hypoxia-responsive genes such as VEGFA, which promotes vascular remodeling and enhances oxygen delivery. These findings collectively offer practical applications for enhancing aquaculture management, such as monitoring biochemical markers, adopting hypoxia-tolerant breeding, and adjusting environmental conditions to mitigate stress, ensuring better productivity and sustainability. This research provides a foundation for further studies on the molecular mechanisms of hypoxia stress in aquaculture species. |
| format | Article |
| id | doaj-art-f1983ae1a31d47f5b91e32775219cf63 |
| institution | Kabale University |
| issn | 2296-7745 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj-art-f1983ae1a31d47f5b91e32775219cf632025-02-05T05:17:50ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452025-02-011210.3389/fmars.2025.15302241530224Effects of hypoxia on the heart of the juvenile four-finger threadfin (Eleutheronema tetradactylum) based on physiological indicators and transcriptome analysisYi Lu0Eric Amenyogbe1Ye- Yang2Zhong-liang Wang3Zhong-liang Wang4Zhong-liang Wang5Jing-hui Jin6Rui-tao Xie7Eric Kwabena Droepenu8Jian-sheng Huang9Jian-sheng Huang10Jian-sheng Huang11Fishery College, Guangdong Ocean University, Zhanjiang, ChinaDepartment of Water Resources and Aquaculture Management, University of Environment and Sustainable Development, Somanya, Eastern Region, GhanaFishery College, Guangdong Ocean University, Zhanjiang, ChinaFishery College, Guangdong Ocean University, Zhanjiang, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang, ChinaGuangdong Marine Fish Science and Technology Innovation Center, Zhanjiang, ChinaFishery College, Guangdong Ocean University, Zhanjiang, ChinaGuangdong Evergreen Feed Industry Co. Ltd, Zhanjiang, ChinaDepartment of Water Resources and Aquaculture Management, University of Environment and Sustainable Development, Somanya, Eastern Region, GhanaFishery College, Guangdong Ocean University, Zhanjiang, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang, ChinaGuangdong Marine Fish Science and Technology Innovation Center, Zhanjiang, ChinaThis study evaluated the effects of hypoxia on the heart of juvenile four-finger threadfin (Eleutheronema tetradactylum) through physiological and transcriptome analysis. Juveniles with an average weight of 122.82 g and length of 24.60 cm were used. Hypoxia significantly increased serum myocardial enzyme activities, including creatine kinase (CK), creatine kinase-MB isoenzyme, lactate dehydrogenase (LDH), and α-hydroxybutyrate dehydrogenase (HDBH). These indicators initially rose and then declined, reflecting cardiac stress and suggesting their potential as early hypoxia biomarkers for real-time aquaculture monitoring. Histological analysis revealed structural damage in myocardial fibers under hypoxia, with increasing severity over time. This underscores the need to minimize oxygen fluctuations to prevent cardiac tissue degeneration. Transcriptome analysis identified upregulated genes involved in cell communication, immune responses, and intracellular signaling, offering potential targets for breeding hypoxia-tolerant species. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis highlighted key pathways such as mitogen-activated protein kinase (MAPK), hypoxia-inducible factor-1 (HIF-1), endocytosis, and phagosome formation. The MAPK pathway plays a critical role in cellular stress responses, including survival, proliferation, and apoptosis. Hypoxia-induced activation of MAPKs like ERK, JNK, and p38 regulates stress-responsive genes. HIF-1 signaling regulates oxygen homeostasis, with HIF-1α stabilizing hypoxia-responsive genes such as VEGFA, which promotes vascular remodeling and enhances oxygen delivery. These findings collectively offer practical applications for enhancing aquaculture management, such as monitoring biochemical markers, adopting hypoxia-tolerant breeding, and adjusting environmental conditions to mitigate stress, ensuring better productivity and sustainability. This research provides a foundation for further studies on the molecular mechanisms of hypoxia stress in aquaculture species.https://www.frontiersin.org/articles/10.3389/fmars.2025.1530224/fullEleutheronema tetradactylumhypoxia stressheart tissuetranscriptomephysiological indicators |
| spellingShingle | Yi Lu Eric Amenyogbe Ye- Yang Zhong-liang Wang Zhong-liang Wang Zhong-liang Wang Jing-hui Jin Rui-tao Xie Eric Kwabena Droepenu Jian-sheng Huang Jian-sheng Huang Jian-sheng Huang Effects of hypoxia on the heart of the juvenile four-finger threadfin (Eleutheronema tetradactylum) based on physiological indicators and transcriptome analysis Frontiers in Marine Science Eleutheronema tetradactylum hypoxia stress heart tissue transcriptome physiological indicators |
| title | Effects of hypoxia on the heart of the juvenile four-finger threadfin (Eleutheronema tetradactylum) based on physiological indicators and transcriptome analysis |
| title_full | Effects of hypoxia on the heart of the juvenile four-finger threadfin (Eleutheronema tetradactylum) based on physiological indicators and transcriptome analysis |
| title_fullStr | Effects of hypoxia on the heart of the juvenile four-finger threadfin (Eleutheronema tetradactylum) based on physiological indicators and transcriptome analysis |
| title_full_unstemmed | Effects of hypoxia on the heart of the juvenile four-finger threadfin (Eleutheronema tetradactylum) based on physiological indicators and transcriptome analysis |
| title_short | Effects of hypoxia on the heart of the juvenile four-finger threadfin (Eleutheronema tetradactylum) based on physiological indicators and transcriptome analysis |
| title_sort | effects of hypoxia on the heart of the juvenile four finger threadfin eleutheronema tetradactylum based on physiological indicators and transcriptome analysis |
| topic | Eleutheronema tetradactylum hypoxia stress heart tissue transcriptome physiological indicators |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2025.1530224/full |
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