Protocol for multiplexed RNAscope-based imaging of mRNAs in whole-mount adult Drosophila brains

Protocol for multiplexed RNAscope-based imaging of mRNAs in whole-mount adult Drosophila brains

Summary: Visualizing the expression of mRNAs using traditional in situ hybridization is often hampered by obstacles including weak signal, high background, and poor probe specificity. Here, we present a protocol utilizing RNAscope (ACD) to overcome these obstacles and detect multiple types of mRNAs...

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Bibliographic Details
Main Authors: Meilin Wu, Vanessa Lambatan, Peng Guo, William J. Joiner
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
single cell
microscopy
gene expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725000218
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