Antimicrobial Efficacy and Cell Adhesion Inhibition of In Situ Synthesized ZnO Nanoparticles/Polyvinyl Alcohol Nanofibrous Membranes
Nanoparticle metal oxides are emerging as a new class of important materials in medical, agricultural, and industrial applications. In this context, free zinc oxide (ZnO) nanoparticles (NPs) have been increasingly shown with broad antimicrobial activities. However, biological properties of immobiliz...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Advances in Condensed Matter Physics |
| Online Access: | http://dx.doi.org/10.1155/2016/6394124 |
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| author | Jian Li Qun Zhang Minjing Xu Changzhu Wu Ping Li |
| author_facet | Jian Li Qun Zhang Minjing Xu Changzhu Wu Ping Li |
| author_sort | Jian Li |
| collection | DOAJ |
| description | Nanoparticle metal oxides are emerging as a new class of important materials in medical, agricultural, and industrial applications. In this context, free zinc oxide (ZnO) nanoparticles (NPs) have been increasingly shown with broad antimicrobial activities. However, biological properties of immobilized ZnO NPs on matrixes like nanofibrous membranes are still limited. In this study, in situ synthesized ZnO NPs/polyvinyl alcohol (PVA) nanofibrous membranes were fabricated by electrospinning with different zinc acetate concentrations. Characterization results indicated that, with 5 mM zinc acetate, uniform size ZnO NPs (~40 nm) were formed and evenly distributed on the membrane surface. The surfaces became more hydrophobic with higher concentration of zinc acetate. ZnO NPs/PVA nanofibrous membranes showed a broad spectrum of antimicrobial activities and cell adhesion inhibiting effects against four microorganisms including Gram-positive Staphylococcus aureus, Gram-negative Escherichia coli, fungi Candida albicans, and spores of Aspergillus niger. Our data revealed that the major antimicrobial mechanism could be attributed to cell membrane damage and cellular internalization of ZnO NPs, while the hydrophobic surface of the membrane primarily contributed to the cell adhesion inhibition. This study suggests that ZnO NPs/PVA nanofibrous membranes could potentially be used as an effective antimicrobial agent to maintain agricultural and food safety. |
| format | Article |
| id | doaj-art-f1949214a43540458a3c21453f4a9db3 |
| institution | Kabale University |
| issn | 1687-8108 1687-8124 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Condensed Matter Physics |
| spelling | doaj-art-f1949214a43540458a3c21453f4a9db32025-02-03T07:26:17ZengWileyAdvances in Condensed Matter Physics1687-81081687-81242016-01-01201610.1155/2016/63941246394124Antimicrobial Efficacy and Cell Adhesion Inhibition of In Situ Synthesized ZnO Nanoparticles/Polyvinyl Alcohol Nanofibrous MembranesJian Li0Qun Zhang1Minjing Xu2Changzhu Wu3Ping Li4School of Life Science and Technology, Tongji University, 1239 Siping Road, Shanghai 200092, ChinaAnhui Agricultural University, 130 West Changjiang Road, Hefei 230036, ChinaSchool of Life Science and Technology, Tongji University, 1239 Siping Road, Shanghai 200092, ChinaInstitute of Microbiology, Technische Universität Dresden, Zellescher Weg 20b, 01217 Dresden, GermanySchool of Life Science and Technology, Tongji University, 1239 Siping Road, Shanghai 200092, ChinaNanoparticle metal oxides are emerging as a new class of important materials in medical, agricultural, and industrial applications. In this context, free zinc oxide (ZnO) nanoparticles (NPs) have been increasingly shown with broad antimicrobial activities. However, biological properties of immobilized ZnO NPs on matrixes like nanofibrous membranes are still limited. In this study, in situ synthesized ZnO NPs/polyvinyl alcohol (PVA) nanofibrous membranes were fabricated by electrospinning with different zinc acetate concentrations. Characterization results indicated that, with 5 mM zinc acetate, uniform size ZnO NPs (~40 nm) were formed and evenly distributed on the membrane surface. The surfaces became more hydrophobic with higher concentration of zinc acetate. ZnO NPs/PVA nanofibrous membranes showed a broad spectrum of antimicrobial activities and cell adhesion inhibiting effects against four microorganisms including Gram-positive Staphylococcus aureus, Gram-negative Escherichia coli, fungi Candida albicans, and spores of Aspergillus niger. Our data revealed that the major antimicrobial mechanism could be attributed to cell membrane damage and cellular internalization of ZnO NPs, while the hydrophobic surface of the membrane primarily contributed to the cell adhesion inhibition. This study suggests that ZnO NPs/PVA nanofibrous membranes could potentially be used as an effective antimicrobial agent to maintain agricultural and food safety.http://dx.doi.org/10.1155/2016/6394124 |
| spellingShingle | Jian Li Qun Zhang Minjing Xu Changzhu Wu Ping Li Antimicrobial Efficacy and Cell Adhesion Inhibition of In Situ Synthesized ZnO Nanoparticles/Polyvinyl Alcohol Nanofibrous Membranes Advances in Condensed Matter Physics |
| title | Antimicrobial Efficacy and Cell Adhesion Inhibition of In Situ Synthesized ZnO Nanoparticles/Polyvinyl Alcohol Nanofibrous Membranes |
| title_full | Antimicrobial Efficacy and Cell Adhesion Inhibition of In Situ Synthesized ZnO Nanoparticles/Polyvinyl Alcohol Nanofibrous Membranes |
| title_fullStr | Antimicrobial Efficacy and Cell Adhesion Inhibition of In Situ Synthesized ZnO Nanoparticles/Polyvinyl Alcohol Nanofibrous Membranes |
| title_full_unstemmed | Antimicrobial Efficacy and Cell Adhesion Inhibition of In Situ Synthesized ZnO Nanoparticles/Polyvinyl Alcohol Nanofibrous Membranes |
| title_short | Antimicrobial Efficacy and Cell Adhesion Inhibition of In Situ Synthesized ZnO Nanoparticles/Polyvinyl Alcohol Nanofibrous Membranes |
| title_sort | antimicrobial efficacy and cell adhesion inhibition of in situ synthesized zno nanoparticles polyvinyl alcohol nanofibrous membranes |
| url | http://dx.doi.org/10.1155/2016/6394124 |
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