Transcriptomic data reveals MYC as an upstream regulator in laying hen follicular recruitment
Understanding the mechanisms of follicular recruitment is essential for improving laying hen and broiler breeder productivity, as it directly influences egg production. Despite advancements in poultry breeding for enhanced egg production, the factors driving successful ovarian follicle maturation re...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124011258 |
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| author | Ashley E Kramer Kathryn M Ellwood Nicole Guarino Cong-Jun Li Aditya Dutta |
| author_facet | Ashley E Kramer Kathryn M Ellwood Nicole Guarino Cong-Jun Li Aditya Dutta |
| author_sort | Ashley E Kramer |
| collection | DOAJ |
| description | Understanding the mechanisms of follicular recruitment is essential for improving laying hen and broiler breeder productivity, as it directly influences egg production. Despite advancements in poultry breeding for enhanced egg production, the factors driving successful ovarian follicle maturation remain inadequately understood. This study investigates the genetic drivers mediating the transition of pre-recruitment follicles to the pre-ovulatory phase, a crucial stage before ovulation. Using RNA sequencing and bioinformatics approaches such as a differential gene expression analysis, we compared pre-recruitment follicles with the recently recruited F5 pre-ovulatory follicle to identify key genes and upstream regulators involved in this transition. Further validation through qRT-PCR confirmed these findings. Using Qiagen's Ingenuity Pathway Analysis we identified MYC proto-oncogene (C-Myc) as a pivotal upstream regulator, controlling genes essential for cell proliferation and differentiation. Additionally, TGFβ1 emerged as a key regulator, influencing pathways involving SMAD3, TNF, and TP53. The study highlights the intricate regulatory network involving MYC and other transcription factors such as CTNNB1, crucial for follicular development. These findings provide valuable insights into the molecular mechanisms governing follicular selection and maturation, which are essential for enhancing egg production efficiency. Future research should explore the roles of MYC, CTNNB1, and other driver genes in follicular development to further understand and improve reproductive efficiency in poultry. |
| format | Article |
| id | doaj-art-f17b1e107ff14932a662da5fde62242a |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-f17b1e107ff14932a662da5fde62242a2025-01-22T05:40:26ZengElsevierPoultry Science0032-57912025-01-011041104547Transcriptomic data reveals MYC as an upstream regulator in laying hen follicular recruitmentAshley E Kramer0Kathryn M Ellwood1Nicole Guarino2Cong-Jun Li3Aditya Dutta4Department of Animal & Food Sciences, University of Delaware, Newark, DE, 19716, USADepartment of Animal & Food Sciences, University of Delaware, Newark, DE, 19716, USADepartments of Animal & Food Sciences and Communication Sciences & Disorders, University of Delaware, Newark, DE, 19716, USAAnimal Genomics and Improvement Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture USDA, Beltsville, MD, 20705, USADepartments of Animal & Food Sciences, Biological Sciences, Medical & Molecular Sciences, and Microbiology Graduate Program, University of Delaware, Newark, DE, 19716, USA; Corresponding author at: University of Delaware, Full address: 050 Townsend Hall, 531 South College Ave, Newark, DE, 19716, USA.Understanding the mechanisms of follicular recruitment is essential for improving laying hen and broiler breeder productivity, as it directly influences egg production. Despite advancements in poultry breeding for enhanced egg production, the factors driving successful ovarian follicle maturation remain inadequately understood. This study investigates the genetic drivers mediating the transition of pre-recruitment follicles to the pre-ovulatory phase, a crucial stage before ovulation. Using RNA sequencing and bioinformatics approaches such as a differential gene expression analysis, we compared pre-recruitment follicles with the recently recruited F5 pre-ovulatory follicle to identify key genes and upstream regulators involved in this transition. Further validation through qRT-PCR confirmed these findings. Using Qiagen's Ingenuity Pathway Analysis we identified MYC proto-oncogene (C-Myc) as a pivotal upstream regulator, controlling genes essential for cell proliferation and differentiation. Additionally, TGFβ1 emerged as a key regulator, influencing pathways involving SMAD3, TNF, and TP53. The study highlights the intricate regulatory network involving MYC and other transcription factors such as CTNNB1, crucial for follicular development. These findings provide valuable insights into the molecular mechanisms governing follicular selection and maturation, which are essential for enhancing egg production efficiency. Future research should explore the roles of MYC, CTNNB1, and other driver genes in follicular development to further understand and improve reproductive efficiency in poultry.http://www.sciencedirect.com/science/article/pii/S0032579124011258Layer henBroiler breederMYCFollicular recruitmentOvary |
| spellingShingle | Ashley E Kramer Kathryn M Ellwood Nicole Guarino Cong-Jun Li Aditya Dutta Transcriptomic data reveals MYC as an upstream regulator in laying hen follicular recruitment Poultry Science Layer hen Broiler breeder MYC Follicular recruitment Ovary |
| title | Transcriptomic data reveals MYC as an upstream regulator in laying hen follicular recruitment |
| title_full | Transcriptomic data reveals MYC as an upstream regulator in laying hen follicular recruitment |
| title_fullStr | Transcriptomic data reveals MYC as an upstream regulator in laying hen follicular recruitment |
| title_full_unstemmed | Transcriptomic data reveals MYC as an upstream regulator in laying hen follicular recruitment |
| title_short | Transcriptomic data reveals MYC as an upstream regulator in laying hen follicular recruitment |
| title_sort | transcriptomic data reveals myc as an upstream regulator in laying hen follicular recruitment |
| topic | Layer hen Broiler breeder MYC Follicular recruitment Ovary |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124011258 |
| work_keys_str_mv | AT ashleyekramer transcriptomicdatarevealsmycasanupstreamregulatorinlayinghenfollicularrecruitment AT kathrynmellwood transcriptomicdatarevealsmycasanupstreamregulatorinlayinghenfollicularrecruitment AT nicoleguarino transcriptomicdatarevealsmycasanupstreamregulatorinlayinghenfollicularrecruitment AT congjunli transcriptomicdatarevealsmycasanupstreamregulatorinlayinghenfollicularrecruitment AT adityadutta transcriptomicdatarevealsmycasanupstreamregulatorinlayinghenfollicularrecruitment |