An In Vitro Study on Prestin Analog Gene in the Bullfrog Hearing Organs
The prestin-based active process in the mammalian outer hair cells (OHCs) is believed to play a crucial role in auditory signal amplification in the cochlea. Prestin belongs to an anion transporter family (SLC26A). It is densely expressed in the OHC lateral plasma membrane and functions as a voltage...
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| Language: | English |
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Wiley
2020-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2020/3570732 |
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| author | Zhongying Wang Minfei Qian Qixuan Wang Huihui Liu Hao Wu Zhiwu Huang |
| author_facet | Zhongying Wang Minfei Qian Qixuan Wang Huihui Liu Hao Wu Zhiwu Huang |
| author_sort | Zhongying Wang |
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| description | The prestin-based active process in the mammalian outer hair cells (OHCs) is believed to play a crucial role in auditory signal amplification in the cochlea. Prestin belongs to an anion transporter family (SLC26A). It is densely expressed in the OHC lateral plasma membrane and functions as a voltage-dependent motor protein. Analog genes can be found in the genome of nonmammalian species, but their functions in hearing are poorly understood. In the present study, we used the gerbil prestin sequence as a template and identified an analog gene in the bullfrog genome. We expressed the gene in a stable cell line (HEK293T) and performed patch-clamp recording. We found that these cells exhibited prominent nonlinear capacitance (NLC), a widely accepted assay for prestin functioning as a motor protein. Upon close examination, the key parameters of this NLC are comparable to that conferred by the gerbil prestin, and nontransfected cells failed to display NLC. Lastly, we performed patch-clamp recording in HCs of all three hearing organs in bullfrog. HCs in both the sacculus and the amphibian papilla exhibited a capacitance profile that is similar to NLC while HCs in the basilar papilla showed no sign of NLC. Whether or not this NLC-like capacitance change is involved in auditory signal amplification certainly requires further examination; our results represent the first and necessary step in revealing possible roles of prestin in the active hearing processes found in many nonmammalian species. |
| format | Article |
| id | doaj-art-f15f8130ab2e4724aebce8c06f2ecac3 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-f15f8130ab2e4724aebce8c06f2ecac32025-02-03T00:58:42ZengWileyNeural Plasticity2090-59041687-54432020-01-01202010.1155/2020/35707323570732An In Vitro Study on Prestin Analog Gene in the Bullfrog Hearing OrgansZhongying Wang0Minfei Qian1Qixuan Wang2Huihui Liu3Hao Wu4Zhiwu Huang5Department of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaThe prestin-based active process in the mammalian outer hair cells (OHCs) is believed to play a crucial role in auditory signal amplification in the cochlea. Prestin belongs to an anion transporter family (SLC26A). It is densely expressed in the OHC lateral plasma membrane and functions as a voltage-dependent motor protein. Analog genes can be found in the genome of nonmammalian species, but their functions in hearing are poorly understood. In the present study, we used the gerbil prestin sequence as a template and identified an analog gene in the bullfrog genome. We expressed the gene in a stable cell line (HEK293T) and performed patch-clamp recording. We found that these cells exhibited prominent nonlinear capacitance (NLC), a widely accepted assay for prestin functioning as a motor protein. Upon close examination, the key parameters of this NLC are comparable to that conferred by the gerbil prestin, and nontransfected cells failed to display NLC. Lastly, we performed patch-clamp recording in HCs of all three hearing organs in bullfrog. HCs in both the sacculus and the amphibian papilla exhibited a capacitance profile that is similar to NLC while HCs in the basilar papilla showed no sign of NLC. Whether or not this NLC-like capacitance change is involved in auditory signal amplification certainly requires further examination; our results represent the first and necessary step in revealing possible roles of prestin in the active hearing processes found in many nonmammalian species.http://dx.doi.org/10.1155/2020/3570732 |
| spellingShingle | Zhongying Wang Minfei Qian Qixuan Wang Huihui Liu Hao Wu Zhiwu Huang An In Vitro Study on Prestin Analog Gene in the Bullfrog Hearing Organs Neural Plasticity |
| title | An In Vitro Study on Prestin Analog Gene in the Bullfrog Hearing Organs |
| title_full | An In Vitro Study on Prestin Analog Gene in the Bullfrog Hearing Organs |
| title_fullStr | An In Vitro Study on Prestin Analog Gene in the Bullfrog Hearing Organs |
| title_full_unstemmed | An In Vitro Study on Prestin Analog Gene in the Bullfrog Hearing Organs |
| title_short | An In Vitro Study on Prestin Analog Gene in the Bullfrog Hearing Organs |
| title_sort | in vitro study on prestin analog gene in the bullfrog hearing organs |
| url | http://dx.doi.org/10.1155/2020/3570732 |
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