Potentially probiotic NPL 1334 strain of Enterococcus durans benefits rats with diet-induced hypercholesterolemia
Abstract Purpose To study the potential of a candidate probiotic strain belonging to the Enterococcus durans species in alleviating hypercholesterolemia and improving the microbial milieu of rat gut. Methods A previously isolated and characterized E. durans strain NPL 1334 was further screened in vi...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | BMC Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12896-024-00943-5 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832594662608601088 |
|---|---|
| author | Hannan Rashid Haseeb Anwar Fakhir Mehmood Baig Imran Mukhtar Tariq Muhammad Arsalan Zaidi |
| author_facet | Hannan Rashid Haseeb Anwar Fakhir Mehmood Baig Imran Mukhtar Tariq Muhammad Arsalan Zaidi |
| author_sort | Hannan Rashid |
| collection | DOAJ |
| description | Abstract Purpose To study the potential of a candidate probiotic strain belonging to the Enterococcus durans species in alleviating hypercholesterolemia and improving the microbial milieu of rat gut. Methods A previously isolated and characterized E. durans strain NPL 1334 was further screened in vitro for its bile salt hydrolyzation and cholesterol assimilation ability. An in vivo trial using diet-induced hypercholesterolemic rats was conducted to evaluate the effects of the administered test probiotic strain on the animal’s blood biochemical parameters such as total cholesterol (TC), high-density lipopolysaccharides (HDL), low-density lipopolysaccharides (LDL), triglycerides (TG), on body weight, oxidative stress markers, and its impact on intestinal and fecal microbiota as well as a histopathological examination of the test animal’s livers. Results E. durans strain showed good bile salt hydrolyzing ability and ample cholesterol assimilation in vitro. Probiotic-fed hypercholesterolemic rats showed significantly lowered cholesterol, triglyceride and LDL levels. The body weight of probiotic-fed rats was reduced as compared to the control. E. durans also stimulated the growth of beneficial LAB in the intestine of experimental rats and did not harm the liver of the experimental rats. Conclusion E. durans can be a natural therapeutic alternative to manage diet-induced hypercholesterolemia and may eventually enhance anti-cholesterolemic therapies. |
| format | Article |
| id | doaj-art-f158ac59b42148e08187a64abcdbe204 |
| institution | Kabale University |
| issn | 1472-6750 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj-art-f158ac59b42148e08187a64abcdbe2042025-01-19T12:29:28ZengBMCBMC Biotechnology1472-67502025-01-0125111310.1186/s12896-024-00943-5Potentially probiotic NPL 1334 strain of Enterococcus durans benefits rats with diet-induced hypercholesterolemiaHannan Rashid0Haseeb Anwar1Fakhir Mehmood Baig2Imran Mukhtar3Tariq Muhammad4Arsalan Zaidi5Pakistan Institute of Engineering and Applied Sciences (PIEAS)Department of Physiology, Government College University (GCUF)Department of Physiology, Government College University (GCUF)Department of Physiology, Government College University (GCUF)Pakistan Institute of Engineering and Applied Sciences (PIEAS)Pakistan Institute of Engineering and Applied Sciences (PIEAS)Abstract Purpose To study the potential of a candidate probiotic strain belonging to the Enterococcus durans species in alleviating hypercholesterolemia and improving the microbial milieu of rat gut. Methods A previously isolated and characterized E. durans strain NPL 1334 was further screened in vitro for its bile salt hydrolyzation and cholesterol assimilation ability. An in vivo trial using diet-induced hypercholesterolemic rats was conducted to evaluate the effects of the administered test probiotic strain on the animal’s blood biochemical parameters such as total cholesterol (TC), high-density lipopolysaccharides (HDL), low-density lipopolysaccharides (LDL), triglycerides (TG), on body weight, oxidative stress markers, and its impact on intestinal and fecal microbiota as well as a histopathological examination of the test animal’s livers. Results E. durans strain showed good bile salt hydrolyzing ability and ample cholesterol assimilation in vitro. Probiotic-fed hypercholesterolemic rats showed significantly lowered cholesterol, triglyceride and LDL levels. The body weight of probiotic-fed rats was reduced as compared to the control. E. durans also stimulated the growth of beneficial LAB in the intestine of experimental rats and did not harm the liver of the experimental rats. Conclusion E. durans can be a natural therapeutic alternative to manage diet-induced hypercholesterolemia and may eventually enhance anti-cholesterolemic therapies.https://doi.org/10.1186/s12896-024-00943-5EnterococcusHypercholesterolemiaGut microbiotaAntioxidant potentialProbiotics |
| spellingShingle | Hannan Rashid Haseeb Anwar Fakhir Mehmood Baig Imran Mukhtar Tariq Muhammad Arsalan Zaidi Potentially probiotic NPL 1334 strain of Enterococcus durans benefits rats with diet-induced hypercholesterolemia BMC Biotechnology Enterococcus Hypercholesterolemia Gut microbiota Antioxidant potential Probiotics |
| title | Potentially probiotic NPL 1334 strain of Enterococcus durans benefits rats with diet-induced hypercholesterolemia |
| title_full | Potentially probiotic NPL 1334 strain of Enterococcus durans benefits rats with diet-induced hypercholesterolemia |
| title_fullStr | Potentially probiotic NPL 1334 strain of Enterococcus durans benefits rats with diet-induced hypercholesterolemia |
| title_full_unstemmed | Potentially probiotic NPL 1334 strain of Enterococcus durans benefits rats with diet-induced hypercholesterolemia |
| title_short | Potentially probiotic NPL 1334 strain of Enterococcus durans benefits rats with diet-induced hypercholesterolemia |
| title_sort | potentially probiotic npl 1334 strain of enterococcus durans benefits rats with diet induced hypercholesterolemia |
| topic | Enterococcus Hypercholesterolemia Gut microbiota Antioxidant potential Probiotics |
| url | https://doi.org/10.1186/s12896-024-00943-5 |
| work_keys_str_mv | AT hannanrashid potentiallyprobioticnpl1334strainofenterococcusduransbenefitsratswithdietinducedhypercholesterolemia AT haseebanwar potentiallyprobioticnpl1334strainofenterococcusduransbenefitsratswithdietinducedhypercholesterolemia AT fakhirmehmoodbaig potentiallyprobioticnpl1334strainofenterococcusduransbenefitsratswithdietinducedhypercholesterolemia AT imranmukhtar potentiallyprobioticnpl1334strainofenterococcusduransbenefitsratswithdietinducedhypercholesterolemia AT tariqmuhammad potentiallyprobioticnpl1334strainofenterococcusduransbenefitsratswithdietinducedhypercholesterolemia AT arsalanzaidi potentiallyprobioticnpl1334strainofenterococcusduransbenefitsratswithdietinducedhypercholesterolemia |