Escherichia coli Strains Isolated from the Uteri Horn, Mouth, and Rectum of Bitches Suffering from Pyometra: Virulence Factors, Antimicrobial Susceptibilities, and Clonal Relationships among Strains
Pyometra is recognized as one of the main causes of disease and death in the bitch, and Escherichia coli is the major pathogen associated with this disease. In this study, 70 E. coli isolates from the uteri horn, mouth, and rectum of bitches suffering from the disease and 43 E. coli isolates from th...
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| Format: | Article |
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Wiley
2014-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2014/979584 |
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| author | Juliana M. A. Agostinho Andressa de Souza Ruben P. Schocken-Iturrino Lívia G. Beraldo Clarissa A. Borges Fernando A. Ávila José M. Marin |
| author_facet | Juliana M. A. Agostinho Andressa de Souza Ruben P. Schocken-Iturrino Lívia G. Beraldo Clarissa A. Borges Fernando A. Ávila José M. Marin |
| author_sort | Juliana M. A. Agostinho |
| collection | DOAJ |
| description | Pyometra is recognized as one of the main causes of disease and death in the bitch, and Escherichia coli is the major pathogen associated with this disease. In this study, 70 E. coli isolates from the uteri horn, mouth, and rectum of bitches suffering from the disease and 43 E. coli isolates from the rectum of clinically healthy bitches were examined for the presence of uropathogenic virulence genes and susceptibility to antimicrobial drugs. DNA profiles of isolates from uteri horn and mouth in bitches with pyometra were compared by REP, ERIC, and BOX-PCR. Virulence gene frequencies detected in isolates from canine pyometra were as follows: 95.7% fim, 27.1% iss, 25.7% hly, 18.5% iuc, and 17.1% usp. Predominant resistance was determined for cephalothin, ampicillin, and nalidixic acid among the isolates from all sites examined. Multidrug resistance was found on ∼50% pyometra isolates. Using the genotypic methods some isolates from uteri, pus, and saliva of the same bitch proved to have identical DNA profiles which is a reason for concern due to the close relationship between household pets and humans. |
| format | Article |
| id | doaj-art-f152598b88f740e69453a573d281529c |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-f152598b88f740e69453a573d281529c2025-02-03T06:05:47ZengWileyInternational Journal of Microbiology1687-918X1687-91982014-01-01201410.1155/2014/979584979584Escherichia coli Strains Isolated from the Uteri Horn, Mouth, and Rectum of Bitches Suffering from Pyometra: Virulence Factors, Antimicrobial Susceptibilities, and Clonal Relationships among StrainsJuliana M. A. Agostinho0Andressa de Souza1Ruben P. Schocken-Iturrino2Lívia G. Beraldo3Clarissa A. Borges4Fernando A. Ávila5José M. Marin6Programa de Microbiologia Agropecuária, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Campus de Jaboticabal, 14884-900 Jaboticabal, SP, BrazilDepartamento de Medicina Veterinária Preventiva, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Campus de Jaboticabal, 14884-900 Jaboticabal, SP, BrazilDepartamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Campus de Jaboticabal, 14884-900 Jaboticabal, SP, BrazilPrograma de Microbiologia Agropecuária, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Campus de Jaboticabal, 14884-900 Jaboticabal, SP, BrazilPrograma de Microbiologia Agropecuária, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Campus de Jaboticabal, 14884-900 Jaboticabal, SP, BrazilDepartamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Campus de Jaboticabal, 14884-900 Jaboticabal, SP, BrazilDepartamento de Morfologia, Fisiologia e Patologia Básica, FORP, Avenida do Café S/N, Campus USP, 14040-904 Ribeirão Preto, SP, BrazilPyometra is recognized as one of the main causes of disease and death in the bitch, and Escherichia coli is the major pathogen associated with this disease. In this study, 70 E. coli isolates from the uteri horn, mouth, and rectum of bitches suffering from the disease and 43 E. coli isolates from the rectum of clinically healthy bitches were examined for the presence of uropathogenic virulence genes and susceptibility to antimicrobial drugs. DNA profiles of isolates from uteri horn and mouth in bitches with pyometra were compared by REP, ERIC, and BOX-PCR. Virulence gene frequencies detected in isolates from canine pyometra were as follows: 95.7% fim, 27.1% iss, 25.7% hly, 18.5% iuc, and 17.1% usp. Predominant resistance was determined for cephalothin, ampicillin, and nalidixic acid among the isolates from all sites examined. Multidrug resistance was found on ∼50% pyometra isolates. Using the genotypic methods some isolates from uteri, pus, and saliva of the same bitch proved to have identical DNA profiles which is a reason for concern due to the close relationship between household pets and humans.http://dx.doi.org/10.1155/2014/979584 |
| spellingShingle | Juliana M. A. Agostinho Andressa de Souza Ruben P. Schocken-Iturrino Lívia G. Beraldo Clarissa A. Borges Fernando A. Ávila José M. Marin Escherichia coli Strains Isolated from the Uteri Horn, Mouth, and Rectum of Bitches Suffering from Pyometra: Virulence Factors, Antimicrobial Susceptibilities, and Clonal Relationships among Strains International Journal of Microbiology |
| title | Escherichia coli Strains Isolated from the Uteri Horn, Mouth, and Rectum of Bitches Suffering from Pyometra: Virulence Factors, Antimicrobial Susceptibilities, and Clonal Relationships among Strains |
| title_full | Escherichia coli Strains Isolated from the Uteri Horn, Mouth, and Rectum of Bitches Suffering from Pyometra: Virulence Factors, Antimicrobial Susceptibilities, and Clonal Relationships among Strains |
| title_fullStr | Escherichia coli Strains Isolated from the Uteri Horn, Mouth, and Rectum of Bitches Suffering from Pyometra: Virulence Factors, Antimicrobial Susceptibilities, and Clonal Relationships among Strains |
| title_full_unstemmed | Escherichia coli Strains Isolated from the Uteri Horn, Mouth, and Rectum of Bitches Suffering from Pyometra: Virulence Factors, Antimicrobial Susceptibilities, and Clonal Relationships among Strains |
| title_short | Escherichia coli Strains Isolated from the Uteri Horn, Mouth, and Rectum of Bitches Suffering from Pyometra: Virulence Factors, Antimicrobial Susceptibilities, and Clonal Relationships among Strains |
| title_sort | escherichia coli strains isolated from the uteri horn mouth and rectum of bitches suffering from pyometra virulence factors antimicrobial susceptibilities and clonal relationships among strains |
| url | http://dx.doi.org/10.1155/2014/979584 |
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