Computer Based Correlation of the Texture of P63 Expressed Nuclei with Histological Tumour Grade, in Laryngeal Carcinomas
Background. P63 immunostaining has been considered as potential prognostic factor in laryngeal cancer. Considering that P63 is mainly nuclear stain, a possible correlation between the texture of P63-stained nuclei and the tumor’s grade could be of value to diagnosis, since this may be related to bio...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2014/963076 |
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| author | Konstantinos Ninos Spiros Kostopoulos Ioannis Kalatzis Panagiota Ravazoula George Sakelaropoulos George Panayiotakis George Economou Dionisis Cavouras |
| author_facet | Konstantinos Ninos Spiros Kostopoulos Ioannis Kalatzis Panagiota Ravazoula George Sakelaropoulos George Panayiotakis George Economou Dionisis Cavouras |
| author_sort | Konstantinos Ninos |
| collection | DOAJ |
| description | Background. P63 immunostaining has been considered as potential prognostic factor in laryngeal cancer. Considering that P63 is mainly nuclear stain, a possible correlation between the texture of P63-stained nuclei and the tumor’s grade could be of value to diagnosis, since this may be related to biologic information imprinted as texture on P63 expressed nuclei. Objective. To investigate the association between P63 stained nuclei and histologic grade in laryngeal tumor lesions. Methods. Biopsy specimens from laryngeal tumour lesions of 55 patients diagnosed with laryngeal squamous cell carcinomas were immunohistochemically (IHC) stained for P63 expression. Four images were digitized from each patient’s IHC specimens. P63 positively expressed nuclei were identified, the percentage of P63 expressed nuclei was computed, and 118 textural, morphological, shape, and architectural features were calculated from each one of the 55 laryngeal lesions. Data were split into the low grade (21 grade I lesions) and high grade (34 grade II and grade III lesions) classes for statistical analysis. Results. With advancing grade, P63 expression decreased, P63 stained nuclei appeared of lower image intensity, more inhomogeneous, of higher local contrast, contained smaller randomly distributed dissimilar structures and had irregular shape. Conclusion. P63 expressed nuclei contain important information related to histologic grade. |
| format | Article |
| id | doaj-art-f14ff96ff64c491cb80dad77337414b0 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-f14ff96ff64c491cb80dad77337414b02025-02-03T06:05:47ZengWileyAnalytical Cellular Pathology2210-71772210-71852014-01-01201410.1155/2014/963076963076Computer Based Correlation of the Texture of P63 Expressed Nuclei with Histological Tumour Grade, in Laryngeal CarcinomasKonstantinos Ninos0Spiros Kostopoulos1Ioannis Kalatzis2Panagiota Ravazoula3George Sakelaropoulos4George Panayiotakis5George Economou6Dionisis Cavouras7Department of Physics, School of Natural Sciences, University of Patras, Rio, 26504 Patras, GreeceMedical Image and Signal Processing Laboratory, Department of Biomedical Engineering, Technological Educational Institute of Athens, Ag. Spyridonos, Egaleo, 12210 Athens, GreeceMedical Image and Signal Processing Laboratory, Department of Biomedical Engineering, Technological Educational Institute of Athens, Ag. Spyridonos, Egaleo, 12210 Athens, GreeceDepartment of Pathology, University Hospital of Patras, 26504 Rio, GreeceDepartment of Medical Physics, School of Health Sciences, Faculty of Medicine, University of Patras, Rio, 26504 Patras, GreeceDepartment of Medical Physics, School of Health Sciences, Faculty of Medicine, University of Patras, Rio, 26504 Patras, GreeceDepartment of Physics, School of Natural Sciences, University of Patras, Rio, 26504 Patras, GreeceMedical Image and Signal Processing Laboratory, Department of Biomedical Engineering, Technological Educational Institute of Athens, Ag. Spyridonos, Egaleo, 12210 Athens, GreeceBackground. P63 immunostaining has been considered as potential prognostic factor in laryngeal cancer. Considering that P63 is mainly nuclear stain, a possible correlation between the texture of P63-stained nuclei and the tumor’s grade could be of value to diagnosis, since this may be related to biologic information imprinted as texture on P63 expressed nuclei. Objective. To investigate the association between P63 stained nuclei and histologic grade in laryngeal tumor lesions. Methods. Biopsy specimens from laryngeal tumour lesions of 55 patients diagnosed with laryngeal squamous cell carcinomas were immunohistochemically (IHC) stained for P63 expression. Four images were digitized from each patient’s IHC specimens. P63 positively expressed nuclei were identified, the percentage of P63 expressed nuclei was computed, and 118 textural, morphological, shape, and architectural features were calculated from each one of the 55 laryngeal lesions. Data were split into the low grade (21 grade I lesions) and high grade (34 grade II and grade III lesions) classes for statistical analysis. Results. With advancing grade, P63 expression decreased, P63 stained nuclei appeared of lower image intensity, more inhomogeneous, of higher local contrast, contained smaller randomly distributed dissimilar structures and had irregular shape. Conclusion. P63 expressed nuclei contain important information related to histologic grade.http://dx.doi.org/10.1155/2014/963076 |
| spellingShingle | Konstantinos Ninos Spiros Kostopoulos Ioannis Kalatzis Panagiota Ravazoula George Sakelaropoulos George Panayiotakis George Economou Dionisis Cavouras Computer Based Correlation of the Texture of P63 Expressed Nuclei with Histological Tumour Grade, in Laryngeal Carcinomas Analytical Cellular Pathology |
| title | Computer Based Correlation of the Texture of P63 Expressed Nuclei with Histological Tumour Grade, in Laryngeal Carcinomas |
| title_full | Computer Based Correlation of the Texture of P63 Expressed Nuclei with Histological Tumour Grade, in Laryngeal Carcinomas |
| title_fullStr | Computer Based Correlation of the Texture of P63 Expressed Nuclei with Histological Tumour Grade, in Laryngeal Carcinomas |
| title_full_unstemmed | Computer Based Correlation of the Texture of P63 Expressed Nuclei with Histological Tumour Grade, in Laryngeal Carcinomas |
| title_short | Computer Based Correlation of the Texture of P63 Expressed Nuclei with Histological Tumour Grade, in Laryngeal Carcinomas |
| title_sort | computer based correlation of the texture of p63 expressed nuclei with histological tumour grade in laryngeal carcinomas |
| url | http://dx.doi.org/10.1155/2014/963076 |
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