Effect of Magnolol on the Function of Osteoblastic MC3T3-E1 Cells
Objectives. In the present study, the ability of magnolol, a hydroxylated biphenyl compound isolated from Magnolia officinalis, to stimulate osteoblast function and inhibit the release of bone-resorbing mediators was investigated in osteoblastic MC3T3-E1 cells. Methods. Osteoblast function was measu...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/829650 |
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| _version_ | 1832552056658853888 |
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| author | Eun Jung Kwak Young Soon Lee Eun Mi Choi |
| author_facet | Eun Jung Kwak Young Soon Lee Eun Mi Choi |
| author_sort | Eun Jung Kwak |
| collection | DOAJ |
| description | Objectives. In the present study, the ability of magnolol, a hydroxylated biphenyl compound isolated from Magnolia officinalis, to stimulate osteoblast function and inhibit the release of bone-resorbing mediators was investigated in osteoblastic MC3T3-E1 cells. Methods. Osteoblast function was measured by cell growth, alkaline phosphatase activity, collagen synthesis, and mineralization. Glutathione content was also measured in the cells. Bone-resorbing cytokines, receptor activator of nuclear factor-κB ligand (RANKL), TNF-α, and IL-6 were measured with an enzyme immunoassay system. Results. Magnolol caused a significant elevation of cell growth, alkaline phosphatase activity, collagen synthesis, mineralization, and glutathione content in the cells (P<0.05). Skeletal turnover is orchestrated by a complex network of regulatory factors. Among cytokines, RANKL, TNF-α, and IL-6 were found to be key osteoclastogenetic molecules produced by osteoblasts. Magnolol significantly (P<0.05) decreased the production of osteoclast differentiation inducing factors such as RANKL, TNF-α, and IL-6 in the presence of antimycin A, which inhibits mitochondrial electron transport and has been used as an ROS generator. Conclusion. Magnolol might be a candidate as an agent for the prevention of bone disorders such as osteoporosis. |
| format | Article |
| id | doaj-art-f128e3a09279424ea175ffae217c0691 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-f128e3a09279424ea175ffae217c06912025-02-03T05:59:52ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/829650829650Effect of Magnolol on the Function of Osteoblastic MC3T3-E1 CellsEun Jung Kwak0Young Soon Lee1Eun Mi Choi2Department of Food Science Technology, Yeungnam University, Gyeongsan 712-749, Republic of KoreaDepartment of Food & Nutrition, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of KoreaDepartment of Food & Nutrition, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of KoreaObjectives. In the present study, the ability of magnolol, a hydroxylated biphenyl compound isolated from Magnolia officinalis, to stimulate osteoblast function and inhibit the release of bone-resorbing mediators was investigated in osteoblastic MC3T3-E1 cells. Methods. Osteoblast function was measured by cell growth, alkaline phosphatase activity, collagen synthesis, and mineralization. Glutathione content was also measured in the cells. Bone-resorbing cytokines, receptor activator of nuclear factor-κB ligand (RANKL), TNF-α, and IL-6 were measured with an enzyme immunoassay system. Results. Magnolol caused a significant elevation of cell growth, alkaline phosphatase activity, collagen synthesis, mineralization, and glutathione content in the cells (P<0.05). Skeletal turnover is orchestrated by a complex network of regulatory factors. Among cytokines, RANKL, TNF-α, and IL-6 were found to be key osteoclastogenetic molecules produced by osteoblasts. Magnolol significantly (P<0.05) decreased the production of osteoclast differentiation inducing factors such as RANKL, TNF-α, and IL-6 in the presence of antimycin A, which inhibits mitochondrial electron transport and has been used as an ROS generator. Conclusion. Magnolol might be a candidate as an agent for the prevention of bone disorders such as osteoporosis.http://dx.doi.org/10.1155/2012/829650 |
| spellingShingle | Eun Jung Kwak Young Soon Lee Eun Mi Choi Effect of Magnolol on the Function of Osteoblastic MC3T3-E1 Cells Mediators of Inflammation |
| title | Effect of Magnolol on the Function of Osteoblastic MC3T3-E1 Cells |
| title_full | Effect of Magnolol on the Function of Osteoblastic MC3T3-E1 Cells |
| title_fullStr | Effect of Magnolol on the Function of Osteoblastic MC3T3-E1 Cells |
| title_full_unstemmed | Effect of Magnolol on the Function of Osteoblastic MC3T3-E1 Cells |
| title_short | Effect of Magnolol on the Function of Osteoblastic MC3T3-E1 Cells |
| title_sort | effect of magnolol on the function of osteoblastic mc3t3 e1 cells |
| url | http://dx.doi.org/10.1155/2012/829650 |
| work_keys_str_mv | AT eunjungkwak effectofmagnololonthefunctionofosteoblasticmc3t3e1cells AT youngsoonlee effectofmagnololonthefunctionofosteoblasticmc3t3e1cells AT eunmichoi effectofmagnololonthefunctionofosteoblasticmc3t3e1cells |