Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation
Aim. To analyze the risks of preoperatively produced donor-specific antibody (DSA) in liver transplantation. Methods. DSA was assessed using direct complement-dependent cytotoxicity (CDC) and anti-human globulin- (AHG-) CDC tests, as well as the Luminex Single Antigen assay. Among 616 patients under...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/972705 |
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| author | Atsushi Yoshizawa Hiroto Egawa Kimiko Yurugi Rie Hishida Hiroaki Tsuji Eiji Ashihara Aya Miyagawa-Hayashino Satoshi Teramukai Taira Maekawa Hironori Haga Sinji Uemoto |
| author_facet | Atsushi Yoshizawa Hiroto Egawa Kimiko Yurugi Rie Hishida Hiroaki Tsuji Eiji Ashihara Aya Miyagawa-Hayashino Satoshi Teramukai Taira Maekawa Hironori Haga Sinji Uemoto |
| author_sort | Atsushi Yoshizawa |
| collection | DOAJ |
| description | Aim. To analyze the risks of preoperatively produced donor-specific antibody (DSA) in liver transplantation. Methods. DSA was assessed using direct complement-dependent cytotoxicity (CDC) and anti-human globulin- (AHG-) CDC tests, as well as the Luminex Single Antigen assay. Among 616 patients undergoing blood type identical or compatible living donor liver transplantation (LDLT), 21 patients were positive for CDC or AHG-CDC tests, and the preserved serum from 18 patients was examined to determine targeted Class I and II antigens. The relationships between the mean fluorescence intensity (MFI) of DSA and the clinical outcomes were analyzed. Results. Patients were divided into 3 groups according to the MFI of anti-Class I DSA: high (11 patients with MFI > 10,000), low (2 patients with MFI < 10,000), and negative (5 patients) MFI groups. Six of 11 patients with high Class-I DSA showed positive Class-II DSA. Hospital death occurred in 7 patients of the high MFI group. High MFI was a significant risk factor for mortality (). Univariate analysis showed a significant correlation between MFI strength and C4d deposition (). Conclusions. HLA Class I DSA with MFI > 10,000 had a significant negative effect on the clinical outcome of patients with preformed DSA in LDLT. |
| format | Article |
| id | doaj-art-f1135cbaddec499c90d92c2f1c68028b |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-f1135cbaddec499c90d92c2f1c68028b2025-02-03T01:30:12ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/972705972705Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver TransplantationAtsushi Yoshizawa0Hiroto Egawa1Kimiko Yurugi2Rie Hishida3Hiroaki Tsuji4Eiji Ashihara5Aya Miyagawa-Hayashino6Satoshi Teramukai7Taira Maekawa8Hironori Haga9Sinji Uemoto10Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, JapanDepartment of Surgery, Institute of Gastroenterology, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, JapanDepartment of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, Kyoto 606-8507, JapanDepartment of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, Kyoto 606-8507, JapanDepartment of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, Kyoto 606-8507, JapanDepartment of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, Kyoto 606-8507, JapanDepartment of Diagnostic Pathology, Kyoto University, Kyoto 606-8507, JapanDivision of Clinical Trial Design and Management, Translational Research Center, Kyoto University Hospital, Kyoto 606-8507, JapanDepartment of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, Kyoto 606-8507, JapanDepartment of Diagnostic Pathology, Kyoto University, Kyoto 606-8507, JapanDepartment of Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, JapanAim. To analyze the risks of preoperatively produced donor-specific antibody (DSA) in liver transplantation. Methods. DSA was assessed using direct complement-dependent cytotoxicity (CDC) and anti-human globulin- (AHG-) CDC tests, as well as the Luminex Single Antigen assay. Among 616 patients undergoing blood type identical or compatible living donor liver transplantation (LDLT), 21 patients were positive for CDC or AHG-CDC tests, and the preserved serum from 18 patients was examined to determine targeted Class I and II antigens. The relationships between the mean fluorescence intensity (MFI) of DSA and the clinical outcomes were analyzed. Results. Patients were divided into 3 groups according to the MFI of anti-Class I DSA: high (11 patients with MFI > 10,000), low (2 patients with MFI < 10,000), and negative (5 patients) MFI groups. Six of 11 patients with high Class-I DSA showed positive Class-II DSA. Hospital death occurred in 7 patients of the high MFI group. High MFI was a significant risk factor for mortality (). Univariate analysis showed a significant correlation between MFI strength and C4d deposition (). Conclusions. HLA Class I DSA with MFI > 10,000 had a significant negative effect on the clinical outcome of patients with preformed DSA in LDLT.http://dx.doi.org/10.1155/2013/972705 |
| spellingShingle | Atsushi Yoshizawa Hiroto Egawa Kimiko Yurugi Rie Hishida Hiroaki Tsuji Eiji Ashihara Aya Miyagawa-Hayashino Satoshi Teramukai Taira Maekawa Hironori Haga Sinji Uemoto Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation Clinical and Developmental Immunology |
| title | Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation |
| title_full | Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation |
| title_fullStr | Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation |
| title_full_unstemmed | Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation |
| title_short | Significance of Semiquantitative Assessment of Preformed Donor-Specific Antibody Using Luminex Single Bead Assay in Living Related Liver Transplantation |
| title_sort | significance of semiquantitative assessment of preformed donor specific antibody using luminex single bead assay in living related liver transplantation |
| url | http://dx.doi.org/10.1155/2013/972705 |
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