Low pH induces amiloride-sensitive expression of leukemia inhibitory factor in endometrial cells
An association has been reported between a lower pH in the uterus and an increased rate of implantation. How low pH regulates endometrial function is unclear. This study investigated the effect of low pH on the expression of leukemia inhibitory factor (LIF), which is crucial for implantation, in a h...
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| Format: | Article |
| Language: | English |
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The Society for Reproduction and Development
2025-03-01
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| Series: | The Journal of Reproduction and Development |
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| Online Access: | https://www.jstage.jst.go.jp/article/jrd/71/3/71_2024-105/_pdf/-char/en |
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| author | Miku OTSUGU Ayumi MINE Kurumi FUJIWARA Ayako ICHIMURA Keiji YAMAMOTO Ryo TACHIHARA Hideaki TOMURA |
| author_facet | Miku OTSUGU Ayumi MINE Kurumi FUJIWARA Ayako ICHIMURA Keiji YAMAMOTO Ryo TACHIHARA Hideaki TOMURA |
| author_sort | Miku OTSUGU |
| collection | DOAJ |
| description | An association has been reported between a lower pH in the uterus and an increased rate of implantation. How low pH regulates endometrial function is unclear. This study investigated the effect of low pH on the expression of leukemia inhibitory factor (LIF), which is crucial for implantation, in a human endometrial carcinoma cell line, rat endometrial stromal cells, and porcine endometrial cells. LIF mRNA expression was quantified by real-time PCR and protein expression was assessed using western blot analysis. LIF mRNA and protein expression increased at low pH in human endometrial carcinoma cells. Increased LIF mRNA expression was also detected at low pH in rat endometrial stromal and porcine endometrial cells, suggesting that low intrauterine pH may create favorable conditions for implantation and endometrial receptivity across species. The increase in LIF mRNA expression in the three cell types was attenuated by the addition of amiloride, indicating that low pH promotes the expression of LIF via amiloride-sensitive molecules in the endometrium. |
| format | Article |
| id | doaj-art-f0f24f86d0d24f16979f76cd6fb25a91 |
| institution | OA Journals |
| issn | 0916-8818 1348-4400 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj-art-f0f24f86d0d24f16979f76cd6fb25a912025-08-20T02:03:18ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002025-03-0171311512310.1262/jrd.2024-105jrdLow pH induces amiloride-sensitive expression of leukemia inhibitory factor in endometrial cellsMiku OTSUGU0Ayumi MINE1Kurumi FUJIWARA2Ayako ICHIMURA3Keiji YAMAMOTO4Ryo TACHIHARA5Hideaki TOMURA6Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, JapanLaboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, JapanLaboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, JapanLaboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, JapanLaboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, JapanLaboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, JapanLaboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, JapanAn association has been reported between a lower pH in the uterus and an increased rate of implantation. How low pH regulates endometrial function is unclear. This study investigated the effect of low pH on the expression of leukemia inhibitory factor (LIF), which is crucial for implantation, in a human endometrial carcinoma cell line, rat endometrial stromal cells, and porcine endometrial cells. LIF mRNA expression was quantified by real-time PCR and protein expression was assessed using western blot analysis. LIF mRNA and protein expression increased at low pH in human endometrial carcinoma cells. Increased LIF mRNA expression was also detected at low pH in rat endometrial stromal and porcine endometrial cells, suggesting that low intrauterine pH may create favorable conditions for implantation and endometrial receptivity across species. The increase in LIF mRNA expression in the three cell types was attenuated by the addition of amiloride, indicating that low pH promotes the expression of LIF via amiloride-sensitive molecules in the endometrium.https://www.jstage.jst.go.jp/article/jrd/71/3/71_2024-105/_pdf/-char/enamilorideendometrial cellleukemia inhibitory factor (lif)low phsodium-proton exchangers (nhes) |
| spellingShingle | Miku OTSUGU Ayumi MINE Kurumi FUJIWARA Ayako ICHIMURA Keiji YAMAMOTO Ryo TACHIHARA Hideaki TOMURA Low pH induces amiloride-sensitive expression of leukemia inhibitory factor in endometrial cells The Journal of Reproduction and Development amiloride endometrial cell leukemia inhibitory factor (lif) low ph sodium-proton exchangers (nhes) |
| title | Low pH induces amiloride-sensitive expression of leukemia inhibitory factor in endometrial cells |
| title_full | Low pH induces amiloride-sensitive expression of leukemia inhibitory factor in endometrial cells |
| title_fullStr | Low pH induces amiloride-sensitive expression of leukemia inhibitory factor in endometrial cells |
| title_full_unstemmed | Low pH induces amiloride-sensitive expression of leukemia inhibitory factor in endometrial cells |
| title_short | Low pH induces amiloride-sensitive expression of leukemia inhibitory factor in endometrial cells |
| title_sort | low ph induces amiloride sensitive expression of leukemia inhibitory factor in endometrial cells |
| topic | amiloride endometrial cell leukemia inhibitory factor (lif) low ph sodium-proton exchangers (nhes) |
| url | https://www.jstage.jst.go.jp/article/jrd/71/3/71_2024-105/_pdf/-char/en |
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