Detection of canine distemper virus (CDV) neutralising antibodies in small ruminants during peste-des-petits-ruminants virus (PPRV) surveillance in Zambia

Abstract Canine distemper virus (CDV) is endemic in Zambia, while peste-des-petits-ruminants virus (PPRV) has not been detected. This study screened 962 farmed goats and 237 small ruminants at informal markets for PPRV antibodies using c-ELISA. A subset (n = 29) was re-analysed with virus neutralisa...

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Main Authors: Sara Lysholm, Nicola Logan, Johanna F Lindahl, Mikael Berg, Elin Johansson, Pernilla Karlsson Bergkvist, George Dautu, Ricky Chazya, Brian J. Willett, Musso Munyeme, Jonas Johansson Wensman
Format: Article
Language:English
Published: BMC 2025-04-01
Series:BMC Veterinary Research
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Online Access:https://doi.org/10.1186/s12917-025-04732-w
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Summary:Abstract Canine distemper virus (CDV) is endemic in Zambia, while peste-des-petits-ruminants virus (PPRV) has not been detected. This study screened 962 farmed goats and 237 small ruminants at informal markets for PPRV antibodies using c-ELISA. A subset (n = 29) was re-analysed with virus neutralisation assays (VNA) for antibodies to PPRV and CDV due to cross-reactivity concerns. c-ELISA indicated 6.44% PPRV seroprevalence on farms and 3.80% at markets. While VNA detected no PPRV antibodies, 45% (13/29) tested positive for CDV, possibly suggesting cross-reactivity. Given the study’s limitations, further research is needed to determine whether PPRV is present in Zambia and assess potential cross-reactivity.
ISSN:1746-6148