Liver-Specific Overexpression of Gamma-Glutamyltransferase Ameliorates Insulin Sensitivity of Male C57BL/6 Mice
In the current study, we developed a liver-specific GGT-overexpressing mice model by rapid injection pLIVE-GGT vector through tail vein and investigated the effects of GGT elevation on glucose metabolism and insulin sensitivity. The serum GGT activity was significantly increased after 7 days of pLIV...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2017/2654520 |
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| _version_ | 1832555143136018432 |
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| author | Yang Long Dan Jia Libin Wei Yumei Yang Haoming Tian Tao Chen |
| author_facet | Yang Long Dan Jia Libin Wei Yumei Yang Haoming Tian Tao Chen |
| author_sort | Yang Long |
| collection | DOAJ |
| description | In the current study, we developed a liver-specific GGT-overexpressing mice model by rapid injection pLIVE-GGT vector through tail vein and investigated the effects of GGT elevation on glucose metabolism and insulin sensitivity. The serum GGT activity was significantly increased after 7 days of pLIVE-GGT1 vector delivery and lasted for about 3 weeks. GGT overexpression reduced the levels of GSSG and GSH in the liver and serum and had no effects on total antioxidative capacity in the liver, kidney, and skeletal muscle except for the pancreas. Increased GGT activity had no effect on the glucose tolerance but could facilitate blood glucose lowering after intraperitoneal insulin administration. The results of Western blotting showed that increased GGT activity enhanced insulin-induced AKT phosphorylation at Ser473. Furthermore, GGT inhibitor could attenuate the changes of insulin-induced blood glucose uptake and AKT phosphorylation in the liver. In summary, the present study developed a liver-specific GGT-overexpressing mice model and found that GGT elevation in short term had no effects on glucose metabolism but could increase insulin sensitivity through enhancing the activity of insulin signaling pathway. |
| format | Article |
| id | doaj-art-f0e750441e4146208a72ee7260c32163 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-f0e750441e4146208a72ee7260c321632025-02-03T05:49:36ZengWileyJournal of Diabetes Research2314-67452314-67532017-01-01201710.1155/2017/26545202654520Liver-Specific Overexpression of Gamma-Glutamyltransferase Ameliorates Insulin Sensitivity of Male C57BL/6 MiceYang Long0Dan Jia1Libin Wei2Yumei Yang3Haoming Tian4Tao Chen5Department of Endocrinology and Metabolism, West China Hospital of Sichuan University, Chengdu 610041, ChinaDivision of General Practice, West China Hospital of Sichuan University, Chengdu 610041, ChinaDepartment of Stomatology, Hebei Medical University Affiliated North China Petroleum Bureau General Hospital, Renqiu 062552, ChinaDepartment of Endocrinology and Metabolism, West China Hospital of Sichuan University, Chengdu 610041, ChinaDepartment of Endocrinology and Metabolism, West China Hospital of Sichuan University, Chengdu 610041, ChinaDepartment of Endocrinology and Metabolism, West China Hospital of Sichuan University, Chengdu 610041, ChinaIn the current study, we developed a liver-specific GGT-overexpressing mice model by rapid injection pLIVE-GGT vector through tail vein and investigated the effects of GGT elevation on glucose metabolism and insulin sensitivity. The serum GGT activity was significantly increased after 7 days of pLIVE-GGT1 vector delivery and lasted for about 3 weeks. GGT overexpression reduced the levels of GSSG and GSH in the liver and serum and had no effects on total antioxidative capacity in the liver, kidney, and skeletal muscle except for the pancreas. Increased GGT activity had no effect on the glucose tolerance but could facilitate blood glucose lowering after intraperitoneal insulin administration. The results of Western blotting showed that increased GGT activity enhanced insulin-induced AKT phosphorylation at Ser473. Furthermore, GGT inhibitor could attenuate the changes of insulin-induced blood glucose uptake and AKT phosphorylation in the liver. In summary, the present study developed a liver-specific GGT-overexpressing mice model and found that GGT elevation in short term had no effects on glucose metabolism but could increase insulin sensitivity through enhancing the activity of insulin signaling pathway.http://dx.doi.org/10.1155/2017/2654520 |
| spellingShingle | Yang Long Dan Jia Libin Wei Yumei Yang Haoming Tian Tao Chen Liver-Specific Overexpression of Gamma-Glutamyltransferase Ameliorates Insulin Sensitivity of Male C57BL/6 Mice Journal of Diabetes Research |
| title | Liver-Specific Overexpression of Gamma-Glutamyltransferase Ameliorates Insulin Sensitivity of Male C57BL/6 Mice |
| title_full | Liver-Specific Overexpression of Gamma-Glutamyltransferase Ameliorates Insulin Sensitivity of Male C57BL/6 Mice |
| title_fullStr | Liver-Specific Overexpression of Gamma-Glutamyltransferase Ameliorates Insulin Sensitivity of Male C57BL/6 Mice |
| title_full_unstemmed | Liver-Specific Overexpression of Gamma-Glutamyltransferase Ameliorates Insulin Sensitivity of Male C57BL/6 Mice |
| title_short | Liver-Specific Overexpression of Gamma-Glutamyltransferase Ameliorates Insulin Sensitivity of Male C57BL/6 Mice |
| title_sort | liver specific overexpression of gamma glutamyltransferase ameliorates insulin sensitivity of male c57bl 6 mice |
| url | http://dx.doi.org/10.1155/2017/2654520 |
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