Serological Survey of Foot-and-Mouth Disease Virus in Buffaloes (Syncerus caffer) in Zambia
A study was conducted to determine the serotypes of foot-and-mouth disease viruses (FMDV) circulating in African buffaloes (Syncerus caffer) from selected areas in Zambia. Sera and probang samples were collected between 2011 and 2012 and analysed for presence of antibodies against FMDV while probang...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2015/264528 |
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| author | T. K. W. Sikombe A. S. Mweene John Muma C. Kasanga Y. Sinkala F. Banda M. Mulumba E. M. Fana C. Mundia M. Simuunza |
| author_facet | T. K. W. Sikombe A. S. Mweene John Muma C. Kasanga Y. Sinkala F. Banda M. Mulumba E. M. Fana C. Mundia M. Simuunza |
| author_sort | T. K. W. Sikombe |
| collection | DOAJ |
| description | A study was conducted to determine the serotypes of foot-and-mouth disease viruses (FMDV) circulating in African buffaloes (Syncerus caffer) from selected areas in Zambia. Sera and probang samples were collected between 2011 and 2012 and analysed for presence of antibodies against FMDV while probang samples were used to isolate the FMDV by observing cytopathic effect (CPE). Samples with CPE were further analysed using antigen ELISA. High FMD seroprevalence was observed and antibodies to all the three Southern African Territories (SAT) serotypes were detected in four study areas represented as follows: SAT2 was 72.7 percent; SAT1 was 62.6 percent; and SAT3 was 26.2 percent. Mixed infections accounted for 68.6 percent of those that were tested positive. For probang samples, CPE were observed in three of the samples, while the antigen ELISA results showed positivity and for SAT1 (n=1) and SAT2 (n=2). It is concluded that FMDV is highly prevalent in Zambian buffaloes which could play an important role in the epidemiology of the disease. Therefore livestock reared at interface with the game parks should be included in all routine FMDV vaccination programmes. |
| format | Article |
| id | doaj-art-f0c96fb2ca72498f9007f7b21ee87ec5 |
| institution | Kabale University |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-f0c96fb2ca72498f9007f7b21ee87ec52025-02-03T01:26:12ZengWileyVeterinary Medicine International2090-81132042-00482015-01-01201510.1155/2015/264528264528Serological Survey of Foot-and-Mouth Disease Virus in Buffaloes (Syncerus caffer) in ZambiaT. K. W. Sikombe0A. S. Mweene1John Muma2C. Kasanga3Y. Sinkala4F. Banda5M. Mulumba6E. M. Fana7C. Mundia8M. Simuunza9Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, ZambiaDepartment of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, ZambiaDepartment of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, ZambiaFaculty of Veterinary Medicine, Sokoine University of Agriculture, P.O. Box 3021, Morogoro, TanzaniaDepartment of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, ZambiaCentral Veterinary Research Institute, P.O. Box 33980, Lusaka, ZambiaSouthern African Development Community Secretariat, SADC House, Plot No. 54385, Central Business District, Private Bag 0095, Gaborone, BotswanaBotswana Vaccine Institute, Private Bag 0031, Gaborone, BotswanaDepartment of Veterinary Services, Southern African Development Community, Trans-Boundary Animal Disease Section, Ministry of Agriculture and Livestock, P.O. Box 50060, Lusaka, ZambiaDepartment of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, ZambiaA study was conducted to determine the serotypes of foot-and-mouth disease viruses (FMDV) circulating in African buffaloes (Syncerus caffer) from selected areas in Zambia. Sera and probang samples were collected between 2011 and 2012 and analysed for presence of antibodies against FMDV while probang samples were used to isolate the FMDV by observing cytopathic effect (CPE). Samples with CPE were further analysed using antigen ELISA. High FMD seroprevalence was observed and antibodies to all the three Southern African Territories (SAT) serotypes were detected in four study areas represented as follows: SAT2 was 72.7 percent; SAT1 was 62.6 percent; and SAT3 was 26.2 percent. Mixed infections accounted for 68.6 percent of those that were tested positive. For probang samples, CPE were observed in three of the samples, while the antigen ELISA results showed positivity and for SAT1 (n=1) and SAT2 (n=2). It is concluded that FMDV is highly prevalent in Zambian buffaloes which could play an important role in the epidemiology of the disease. Therefore livestock reared at interface with the game parks should be included in all routine FMDV vaccination programmes.http://dx.doi.org/10.1155/2015/264528 |
| spellingShingle | T. K. W. Sikombe A. S. Mweene John Muma C. Kasanga Y. Sinkala F. Banda M. Mulumba E. M. Fana C. Mundia M. Simuunza Serological Survey of Foot-and-Mouth Disease Virus in Buffaloes (Syncerus caffer) in Zambia Veterinary Medicine International |
| title | Serological Survey of Foot-and-Mouth Disease Virus in Buffaloes (Syncerus caffer) in Zambia |
| title_full | Serological Survey of Foot-and-Mouth Disease Virus in Buffaloes (Syncerus caffer) in Zambia |
| title_fullStr | Serological Survey of Foot-and-Mouth Disease Virus in Buffaloes (Syncerus caffer) in Zambia |
| title_full_unstemmed | Serological Survey of Foot-and-Mouth Disease Virus in Buffaloes (Syncerus caffer) in Zambia |
| title_short | Serological Survey of Foot-and-Mouth Disease Virus in Buffaloes (Syncerus caffer) in Zambia |
| title_sort | serological survey of foot and mouth disease virus in buffaloes syncerus caffer in zambia |
| url | http://dx.doi.org/10.1155/2015/264528 |
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