Multi-omics revealed antibacterial mechanisms of licochalcone A against MRSA and its antimicrobic potential on pork meat
Licorice flavonoids (LFs) exhibit potent antibacterial activities against Gram-positive bacteria. However, the related mechanism remains unclear. This study aims to illustrate the mechanisms of licochalcone A (LA), a main flavonoid in LFs, against methicillin-resistant Staphylococcus aureus (MRSA)....
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
|
| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524007818 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850109110251421696 |
|---|---|
| author | Fei Zeng Shijuan Shao Zhilu Zou Siqi Guo Yu Cai Chunchao Yan Yunzhong Chen Maolin Wang Tingting Shi |
| author_facet | Fei Zeng Shijuan Shao Zhilu Zou Siqi Guo Yu Cai Chunchao Yan Yunzhong Chen Maolin Wang Tingting Shi |
| author_sort | Fei Zeng |
| collection | DOAJ |
| description | Licorice flavonoids (LFs) exhibit potent antibacterial activities against Gram-positive bacteria. However, the related mechanism remains unclear. This study aims to illustrate the mechanisms of licochalcone A (LA), a main flavonoid in LFs, against methicillin-resistant Staphylococcus aureus (MRSA). The anti-MRSA effect of LA was comprehensively investigated by a combination of proteomics and metabolomics studies. Meanwhile, LA was loaded in glycyrrhizin (GA) micelles (GA@LA micelles) to improve its water solubility. The results demonstrated that LA could disrupt the arginine metabolism and cause the accumulation of intracellular ROS in MRSA. In addition, LA could inhibit the expression of glucokinase in MRSA, which affect the synthesis of ATP, fatty acids, and peptidoglycan. GA@LA micelles have the latent ability to inhibit the growth of MRSA on fresh pork. |
| format | Article |
| id | doaj-art-f0b2806cebfe4714a975f317526b4c86 |
| institution | OA Journals |
| issn | 2590-1575 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-f0b2806cebfe4714a975f317526b4c862025-08-20T02:38:10ZengElsevierFood Chemistry: X2590-15752024-12-012410189310.1016/j.fochx.2024.101893Multi-omics revealed antibacterial mechanisms of licochalcone A against MRSA and its antimicrobic potential on pork meatFei Zeng0Shijuan Shao1Zhilu Zou2Siqi Guo3Yu Cai4Chunchao Yan5Yunzhong Chen6Maolin Wang7Tingting Shi8Faculty of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430070, ChinaFaculty of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430070, ChinaFaculty of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430070, ChinaFaculty of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430070, ChinaFaculty of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430070, ChinaFaculty of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430070, ChinaFaculty of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430070, ChinaInstitute of Traditional Chinese Medicine Health Industry, China Academy of Chinese Medical Sciences, Nanchang 330115, China; Corresponding author.Department of Human Anatomy and Histoembryology, Nanjing University of Chinese Medicine, Nanjing 210023, China; Corresponding author at: Nanjing University of Chinese Medicine, Xianlin Road, Qixia District, Nanjing City, China.Licorice flavonoids (LFs) exhibit potent antibacterial activities against Gram-positive bacteria. However, the related mechanism remains unclear. This study aims to illustrate the mechanisms of licochalcone A (LA), a main flavonoid in LFs, against methicillin-resistant Staphylococcus aureus (MRSA). The anti-MRSA effect of LA was comprehensively investigated by a combination of proteomics and metabolomics studies. Meanwhile, LA was loaded in glycyrrhizin (GA) micelles (GA@LA micelles) to improve its water solubility. The results demonstrated that LA could disrupt the arginine metabolism and cause the accumulation of intracellular ROS in MRSA. In addition, LA could inhibit the expression of glucokinase in MRSA, which affect the synthesis of ATP, fatty acids, and peptidoglycan. GA@LA micelles have the latent ability to inhibit the growth of MRSA on fresh pork.http://www.sciencedirect.com/science/article/pii/S2590157524007818Licorice flavonoidsMethicillin-resistant Staphylococcus aureusMicellesGlycyrrhizin |
| spellingShingle | Fei Zeng Shijuan Shao Zhilu Zou Siqi Guo Yu Cai Chunchao Yan Yunzhong Chen Maolin Wang Tingting Shi Multi-omics revealed antibacterial mechanisms of licochalcone A against MRSA and its antimicrobic potential on pork meat Food Chemistry: X Licorice flavonoids Methicillin-resistant Staphylococcus aureus Micelles Glycyrrhizin |
| title | Multi-omics revealed antibacterial mechanisms of licochalcone A against MRSA and its antimicrobic potential on pork meat |
| title_full | Multi-omics revealed antibacterial mechanisms of licochalcone A against MRSA and its antimicrobic potential on pork meat |
| title_fullStr | Multi-omics revealed antibacterial mechanisms of licochalcone A against MRSA and its antimicrobic potential on pork meat |
| title_full_unstemmed | Multi-omics revealed antibacterial mechanisms of licochalcone A against MRSA and its antimicrobic potential on pork meat |
| title_short | Multi-omics revealed antibacterial mechanisms of licochalcone A against MRSA and its antimicrobic potential on pork meat |
| title_sort | multi omics revealed antibacterial mechanisms of licochalcone a against mrsa and its antimicrobic potential on pork meat |
| topic | Licorice flavonoids Methicillin-resistant Staphylococcus aureus Micelles Glycyrrhizin |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524007818 |
| work_keys_str_mv | AT feizeng multiomicsrevealedantibacterialmechanismsoflicochalconeaagainstmrsaanditsantimicrobicpotentialonporkmeat AT shijuanshao multiomicsrevealedantibacterialmechanismsoflicochalconeaagainstmrsaanditsantimicrobicpotentialonporkmeat AT zhiluzou multiomicsrevealedantibacterialmechanismsoflicochalconeaagainstmrsaanditsantimicrobicpotentialonporkmeat AT siqiguo multiomicsrevealedantibacterialmechanismsoflicochalconeaagainstmrsaanditsantimicrobicpotentialonporkmeat AT yucai multiomicsrevealedantibacterialmechanismsoflicochalconeaagainstmrsaanditsantimicrobicpotentialonporkmeat AT chunchaoyan multiomicsrevealedantibacterialmechanismsoflicochalconeaagainstmrsaanditsantimicrobicpotentialonporkmeat AT yunzhongchen multiomicsrevealedantibacterialmechanismsoflicochalconeaagainstmrsaanditsantimicrobicpotentialonporkmeat AT maolinwang multiomicsrevealedantibacterialmechanismsoflicochalconeaagainstmrsaanditsantimicrobicpotentialonporkmeat AT tingtingshi multiomicsrevealedantibacterialmechanismsoflicochalconeaagainstmrsaanditsantimicrobicpotentialonporkmeat |