Rosavin exerts an antitumor role and inactivates the MAPK/ERK pathway in small-cell lung carcinoma in vitro
This study attempts to explore the function and mechanism of action of rosavin in small-cell lung cancer (SCLC) in vitro. The viability and clone formation of SCLC cells were assessed using cell counting kit-8 and colony formation assays, respectively. Apoptosis and cell cycle were detected using fl...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2023-06-01
|
| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acph-2023-0015 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832570040305582080 |
|---|---|
| author | Liu Rui Jiang Cuihong Zhao Zhizheng Lv Yutong Wang Gaoxing |
| author_facet | Liu Rui Jiang Cuihong Zhao Zhizheng Lv Yutong Wang Gaoxing |
| author_sort | Liu Rui |
| collection | DOAJ |
| description | This study attempts to explore the function and mechanism of action of rosavin in small-cell lung cancer (SCLC) in vitro. The viability and clone formation of SCLC cells were assessed using cell counting kit-8 and colony formation assays, respectively. Apoptosis and cell cycle were detected using flow cytometry and cell cycle analysis, respectively. Wound healing and transwell assays were performed to evaluate the migration and invasion of SCLC cells. Besides, protein levels of p-ERK, ERK, p-MEK and MEK were determined using Western blot analysis. Rosavin repressed the viability and clone formation of SCLC cells, and promoted apoptosis and G0/G1 arrest of SCLC cells. At the same time, rosavin suppressed migration and invasion of SCLC cells. Moreover, protein levels of p-ERK/ERK and p-MEK/MEK were decreased after rosavin addition in SCLC cells. Rosavin impaired malignant behaviors of SCLC cells, which may be associated with inhibition of the MAPK/ERK pathway in vitro. |
| format | Article |
| id | doaj-art-f093bb67d19047b094b889e9ee136294 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2023-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-f093bb67d19047b094b889e9ee1362942025-02-02T17:57:33ZengSciendoActa Pharmaceutica1846-95582023-06-0173226928010.2478/acph-2023-0015Rosavin exerts an antitumor role and inactivates the MAPK/ERK pathway in small-cell lung carcinoma in vitroLiu Rui0Jiang Cuihong1Zhao Zhizheng2Lv Yutong3Wang Gaoxing41Department of Oncology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences (South Campus)Beijing102618, China1Department of Oncology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences (South Campus)Beijing102618, China1Department of Oncology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences (South Campus)Beijing102618, China1Department of Oncology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences (South Campus)Beijing102618, China1Department of Oncology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences (South Campus)Beijing102618, ChinaThis study attempts to explore the function and mechanism of action of rosavin in small-cell lung cancer (SCLC) in vitro. The viability and clone formation of SCLC cells were assessed using cell counting kit-8 and colony formation assays, respectively. Apoptosis and cell cycle were detected using flow cytometry and cell cycle analysis, respectively. Wound healing and transwell assays were performed to evaluate the migration and invasion of SCLC cells. Besides, protein levels of p-ERK, ERK, p-MEK and MEK were determined using Western blot analysis. Rosavin repressed the viability and clone formation of SCLC cells, and promoted apoptosis and G0/G1 arrest of SCLC cells. At the same time, rosavin suppressed migration and invasion of SCLC cells. Moreover, protein levels of p-ERK/ERK and p-MEK/MEK were decreased after rosavin addition in SCLC cells. Rosavin impaired malignant behaviors of SCLC cells, which may be associated with inhibition of the MAPK/ERK pathway in vitro.https://doi.org/10.2478/acph-2023-0015small-cell lung cancerrosavinmekerk |
| spellingShingle | Liu Rui Jiang Cuihong Zhao Zhizheng Lv Yutong Wang Gaoxing Rosavin exerts an antitumor role and inactivates the MAPK/ERK pathway in small-cell lung carcinoma in vitro Acta Pharmaceutica small-cell lung cancer rosavin mek erk |
| title | Rosavin exerts an antitumor role and inactivates the MAPK/ERK pathway in small-cell lung carcinoma in vitro |
| title_full | Rosavin exerts an antitumor role and inactivates the MAPK/ERK pathway in small-cell lung carcinoma in vitro |
| title_fullStr | Rosavin exerts an antitumor role and inactivates the MAPK/ERK pathway in small-cell lung carcinoma in vitro |
| title_full_unstemmed | Rosavin exerts an antitumor role and inactivates the MAPK/ERK pathway in small-cell lung carcinoma in vitro |
| title_short | Rosavin exerts an antitumor role and inactivates the MAPK/ERK pathway in small-cell lung carcinoma in vitro |
| title_sort | rosavin exerts an antitumor role and inactivates the mapk erk pathway in small cell lung carcinoma in vitro |
| topic | small-cell lung cancer rosavin mek erk |
| url | https://doi.org/10.2478/acph-2023-0015 |
| work_keys_str_mv | AT liurui rosavinexertsanantitumorroleandinactivatesthemapkerkpathwayinsmallcelllungcarcinomainvitro AT jiangcuihong rosavinexertsanantitumorroleandinactivatesthemapkerkpathwayinsmallcelllungcarcinomainvitro AT zhaozhizheng rosavinexertsanantitumorroleandinactivatesthemapkerkpathwayinsmallcelllungcarcinomainvitro AT lvyutong rosavinexertsanantitumorroleandinactivatesthemapkerkpathwayinsmallcelllungcarcinomainvitro AT wanggaoxing rosavinexertsanantitumorroleandinactivatesthemapkerkpathwayinsmallcelllungcarcinomainvitro |