Comparative lipidome and transcriptome provide novel insights into zero-valent iron nanoparticle-treated Fremyella diplosiphon
Abstract Understanding the intricate interplay between nanoparticle-mediated cyanobacterial interactions is pivotal in elucidating their impact on the transcriptome and lipidome. In the present study, total fatty acid methyl esters (FAMEs) in the wild-type (B481-WT) and transformant (B481-SD) Fremye...
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Nature Portfolio
2024-11-01
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| Online Access: | https://doi.org/10.1038/s41598-024-79780-7 |
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| author | Yavuz S. Yalcin Samson Gichuki Huan Chen Anithachristy Sigamani Arumanayagam Shyama Malika Malwalage Viji Sitther |
| author_facet | Yavuz S. Yalcin Samson Gichuki Huan Chen Anithachristy Sigamani Arumanayagam Shyama Malika Malwalage Viji Sitther |
| author_sort | Yavuz S. Yalcin |
| collection | DOAJ |
| description | Abstract Understanding the intricate interplay between nanoparticle-mediated cyanobacterial interactions is pivotal in elucidating their impact on the transcriptome and lipidome. In the present study, total fatty acid methyl esters (FAMEs) in the wild-type (B481-WT) and transformant (B481-SD) Fremyella diplosiphon strains treated with nanoscale zero-valent iron nanoparticles (nZVIs) were characterized, and transcriptome changes analyzed. Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry revealed a 20–25% higher percentage of FAMEs in nZVI-treated F. diplosiphon strain B481-SD compared to B481-WT. Accumulation of alkanes was significantly higher (> 1.4 times) in both strains treated with 25.6 mg L−1 nZVIs compared to the untreated control. In addition, we observed significantly higher levels of monounsaturated FAMEs (11%) in B481-WT in 3.2 (11.34%) and 25.6 mg L−1 (11.22%) nZVI-treated cells when compared to the untreated control (7%). Analysis of the F. diplosiphon transcriptome treated with 3.2 mg L−1 revealed a total of 1811 and 1651 genes that were differentially expressed in B481-SD and B481-WT respectively. While the expression of iron uptake and ion channel genes was downregulated, genes coding for photosynthesis, pigment, and antioxidant enzymes were significantly (p < 0.05) upregulated in B481-SD treated with 3.2 mg L−1 nZVIs compared to the untreated control. This study on essential FAMEs and regulation of genes in nZVI-treated F. diplosiphon strains provides a molecular framework for optimization of metabolic pathways in this model species. |
| format | Article |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-f061dcc8155049769f9bb841c4f171a02025-02-02T12:25:34ZengNature PortfolioScientific Reports2045-23222024-11-0114111410.1038/s41598-024-79780-7Comparative lipidome and transcriptome provide novel insights into zero-valent iron nanoparticle-treated Fremyella diplosiphonYavuz S. Yalcin0Samson Gichuki1Huan Chen2Anithachristy Sigamani Arumanayagam3Shyama Malika Malwalage4Viji Sitther5Department of Biology, Morgan State UniversityDepartment of Biology, Morgan State UniversityNational High Magnetic Field Laboratory, Ion Cyclotron Resonance Facility, Florida State UniversityDepartment of Pathology and Genomic Medicine, Houston Methodist Hospital Research InstituteDepartment of Biology, Morgan State UniversityDepartment of Biology, Morgan State UniversityAbstract Understanding the intricate interplay between nanoparticle-mediated cyanobacterial interactions is pivotal in elucidating their impact on the transcriptome and lipidome. In the present study, total fatty acid methyl esters (FAMEs) in the wild-type (B481-WT) and transformant (B481-SD) Fremyella diplosiphon strains treated with nanoscale zero-valent iron nanoparticles (nZVIs) were characterized, and transcriptome changes analyzed. Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry revealed a 20–25% higher percentage of FAMEs in nZVI-treated F. diplosiphon strain B481-SD compared to B481-WT. Accumulation of alkanes was significantly higher (> 1.4 times) in both strains treated with 25.6 mg L−1 nZVIs compared to the untreated control. In addition, we observed significantly higher levels of monounsaturated FAMEs (11%) in B481-WT in 3.2 (11.34%) and 25.6 mg L−1 (11.22%) nZVI-treated cells when compared to the untreated control (7%). Analysis of the F. diplosiphon transcriptome treated with 3.2 mg L−1 revealed a total of 1811 and 1651 genes that were differentially expressed in B481-SD and B481-WT respectively. While the expression of iron uptake and ion channel genes was downregulated, genes coding for photosynthesis, pigment, and antioxidant enzymes were significantly (p < 0.05) upregulated in B481-SD treated with 3.2 mg L−1 nZVIs compared to the untreated control. This study on essential FAMEs and regulation of genes in nZVI-treated F. diplosiphon strains provides a molecular framework for optimization of metabolic pathways in this model species.https://doi.org/10.1038/s41598-024-79780-7CyanobacteriaLipidomeNanoparticlesTranscriptome |
| spellingShingle | Yavuz S. Yalcin Samson Gichuki Huan Chen Anithachristy Sigamani Arumanayagam Shyama Malika Malwalage Viji Sitther Comparative lipidome and transcriptome provide novel insights into zero-valent iron nanoparticle-treated Fremyella diplosiphon Scientific Reports Cyanobacteria Lipidome Nanoparticles Transcriptome |
| title | Comparative lipidome and transcriptome provide novel insights into zero-valent iron nanoparticle-treated Fremyella diplosiphon |
| title_full | Comparative lipidome and transcriptome provide novel insights into zero-valent iron nanoparticle-treated Fremyella diplosiphon |
| title_fullStr | Comparative lipidome and transcriptome provide novel insights into zero-valent iron nanoparticle-treated Fremyella diplosiphon |
| title_full_unstemmed | Comparative lipidome and transcriptome provide novel insights into zero-valent iron nanoparticle-treated Fremyella diplosiphon |
| title_short | Comparative lipidome and transcriptome provide novel insights into zero-valent iron nanoparticle-treated Fremyella diplosiphon |
| title_sort | comparative lipidome and transcriptome provide novel insights into zero valent iron nanoparticle treated fremyella diplosiphon |
| topic | Cyanobacteria Lipidome Nanoparticles Transcriptome |
| url | https://doi.org/10.1038/s41598-024-79780-7 |
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