The Isolation Rate of Escherichia coli 0157:H7 in Toronto and Surrounding Communities

Verocytotoxin-producing strains of Escherichia coli, most often serotype 0157:H7, have been associated with both sporadic and epidemic diarrheal disease in Canada. In order to determine the isolation rate of E coli 0157:H7 in outpatients with diarrhea, all stool specimens submitted for culture to Me...

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Main Authors: Andrew E Simor, Christine Watt, Donald E Low
Format: Article
Language:English
Published: Wiley 1990-01-01
Series:Canadian Journal of Infectious Diseases
Online Access:http://dx.doi.org/10.1155/1990/583209
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author Andrew E Simor
Christine Watt
Donald E Low
author_facet Andrew E Simor
Christine Watt
Donald E Low
author_sort Andrew E Simor
collection DOAJ
description Verocytotoxin-producing strains of Escherichia coli, most often serotype 0157:H7, have been associated with both sporadic and epidemic diarrheal disease in Canada. In order to determine the isolation rate of E coli 0157:H7 in outpatients with diarrhea, all stool specimens submitted for culture to Med-Chem Laboratories in Metropolitan Toronto between June 1988 and September 1989 were cultured on MacConkey-Sorbitol agar in addition to standard enteric media. A total of 46 (0.3%) of 16,125 stool specimens yielded E coli 0157:H7 or verotoxin-producing E coli 0157:H−. These isolates came from 31 patients with diarrhea; only 16 (52%) had a history of hemorrhagic colitis and one patient developed hemolytic uremic syndrome. Although MacConkey-Sorbitol agar was useful as a differential medium for detecting E coli 0157:H7, 14.5% of all specimens yielded nonsorbitol-fermenting isolates. It is not certain whether the routine use of MacConkey-Sorbitol agar is justified when isolation rates of E coli 0157:117 are very low.
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spelling doaj-art-f016f29cefd847b6a217c8c6d5b4ed5b2025-08-20T03:06:00ZengWileyCanadian Journal of Infectious Diseases1180-23321990-01-0111232710.1155/1990/583209The Isolation Rate of Escherichia coli 0157:H7 in Toronto and Surrounding CommunitiesAndrew E Simor0Christine WattDonald E Low1Department of Microbiology, Mount Sinai Hospital, Toronto, Ontario, CanadaDepartment of Microbiology, Mount Sinai Hospital, Toronto, Ontario, CanadaVerocytotoxin-producing strains of Escherichia coli, most often serotype 0157:H7, have been associated with both sporadic and epidemic diarrheal disease in Canada. In order to determine the isolation rate of E coli 0157:H7 in outpatients with diarrhea, all stool specimens submitted for culture to Med-Chem Laboratories in Metropolitan Toronto between June 1988 and September 1989 were cultured on MacConkey-Sorbitol agar in addition to standard enteric media. A total of 46 (0.3%) of 16,125 stool specimens yielded E coli 0157:H7 or verotoxin-producing E coli 0157:H−. These isolates came from 31 patients with diarrhea; only 16 (52%) had a history of hemorrhagic colitis and one patient developed hemolytic uremic syndrome. Although MacConkey-Sorbitol agar was useful as a differential medium for detecting E coli 0157:H7, 14.5% of all specimens yielded nonsorbitol-fermenting isolates. It is not certain whether the routine use of MacConkey-Sorbitol agar is justified when isolation rates of E coli 0157:117 are very low.http://dx.doi.org/10.1155/1990/583209
spellingShingle Andrew E Simor
Christine Watt
Donald E Low
The Isolation Rate of Escherichia coli 0157:H7 in Toronto and Surrounding Communities
Canadian Journal of Infectious Diseases
title The Isolation Rate of Escherichia coli 0157:H7 in Toronto and Surrounding Communities
title_full The Isolation Rate of Escherichia coli 0157:H7 in Toronto and Surrounding Communities
title_fullStr The Isolation Rate of Escherichia coli 0157:H7 in Toronto and Surrounding Communities
title_full_unstemmed The Isolation Rate of Escherichia coli 0157:H7 in Toronto and Surrounding Communities
title_short The Isolation Rate of Escherichia coli 0157:H7 in Toronto and Surrounding Communities
title_sort isolation rate of escherichia coli 0157 h7 in toronto and surrounding communities
url http://dx.doi.org/10.1155/1990/583209
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