Modulation of Vasomotive Activity in Rabbit External Ophthalmic Artery by Neuropeptides
Purpose. To investigate the vasomotive activity upon the external ophthalmic artery of vasointestinal peptide (VIP) and neuropeptide Y (NPY) using a previously developed model. Methods. Isolated rabbit eyes (n=12) were perfused in situ with tyrode through the external ophthalmic artery. Effects of i...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2012/498565 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832546098495881216 |
|---|---|
| author | Esmeralda Sofia Costa Delgado Carlos Marques-Neves Maria Isabel Sousa Rocha José Paulo Pacheco Sales-Luís Luís Filipe Silva-Carvalho |
| author_facet | Esmeralda Sofia Costa Delgado Carlos Marques-Neves Maria Isabel Sousa Rocha José Paulo Pacheco Sales-Luís Luís Filipe Silva-Carvalho |
| author_sort | Esmeralda Sofia Costa Delgado |
| collection | DOAJ |
| description | Purpose. To investigate the vasomotive activity upon the external ophthalmic artery of vasointestinal peptide (VIP) and neuropeptide Y (NPY) using a previously developed model.
Methods. Isolated rabbit eyes (n=12) were perfused in situ with tyrode through the external ophthalmic artery. Effects of intra-arterial injections of NPY 200 μg/ml (Group A; n=6) and VIP 200 μg/ml (Group B; n=6) on the recorded pressure were obtained. For statistical analysis, Student's paired t-test and Fast Fourier Transform were used.
Results. Spontaneous oscillations were observed before any drug administration in the 12 rabbit models. NPY produced an increase in total vascular resistance and a higher frequency and amplitude of oscillations, while VIP evoked the opposite effects.
Conclusions. This study provides evidence of vasomotion in basal conditions in rabbit external ophthalmic artery. Concerning drug effects, NPY increased arterial resistance and enhanced vasomotion while VIP produced opposite effects which demonstrates their profound influence in arterial vasomotion. |
| format | Article |
| id | doaj-art-f0121c81836047d49330a5a82e9def3a |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-f0121c81836047d49330a5a82e9def3a2025-02-03T07:23:53ZengWileyJournal of Ophthalmology2090-004X2090-00582012-01-01201210.1155/2012/498565498565Modulation of Vasomotive Activity in Rabbit External Ophthalmic Artery by NeuropeptidesEsmeralda Sofia Costa Delgado0Carlos Marques-Neves1Maria Isabel Sousa Rocha2José Paulo Pacheco Sales-Luís3Luís Filipe Silva-Carvalho4Clinical Department, CIISA, Faculty of Veterinary Medicine, Technical University of Lisbon, Alameda da Universidade Técnica, 1300-477 Lisbon, PortugalInstituto de Medicina Molecular and Instituto de Fisiologia, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028 Lisbon, PortugalInstituto de Medicina Molecular and Instituto de Fisiologia, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028 Lisbon, PortugalClinical Department, CIISA, Faculty of Veterinary Medicine, Technical University of Lisbon, Alameda da Universidade Técnica, 1300-477 Lisbon, PortugalInstituto de Medicina Molecular and Instituto de Fisiologia, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028 Lisbon, PortugalPurpose. To investigate the vasomotive activity upon the external ophthalmic artery of vasointestinal peptide (VIP) and neuropeptide Y (NPY) using a previously developed model. Methods. Isolated rabbit eyes (n=12) were perfused in situ with tyrode through the external ophthalmic artery. Effects of intra-arterial injections of NPY 200 μg/ml (Group A; n=6) and VIP 200 μg/ml (Group B; n=6) on the recorded pressure were obtained. For statistical analysis, Student's paired t-test and Fast Fourier Transform were used. Results. Spontaneous oscillations were observed before any drug administration in the 12 rabbit models. NPY produced an increase in total vascular resistance and a higher frequency and amplitude of oscillations, while VIP evoked the opposite effects. Conclusions. This study provides evidence of vasomotion in basal conditions in rabbit external ophthalmic artery. Concerning drug effects, NPY increased arterial resistance and enhanced vasomotion while VIP produced opposite effects which demonstrates their profound influence in arterial vasomotion.http://dx.doi.org/10.1155/2012/498565 |
| spellingShingle | Esmeralda Sofia Costa Delgado Carlos Marques-Neves Maria Isabel Sousa Rocha José Paulo Pacheco Sales-Luís Luís Filipe Silva-Carvalho Modulation of Vasomotive Activity in Rabbit External Ophthalmic Artery by Neuropeptides Journal of Ophthalmology |
| title | Modulation of Vasomotive Activity in Rabbit External Ophthalmic
Artery by Neuropeptides |
| title_full | Modulation of Vasomotive Activity in Rabbit External Ophthalmic
Artery by Neuropeptides |
| title_fullStr | Modulation of Vasomotive Activity in Rabbit External Ophthalmic
Artery by Neuropeptides |
| title_full_unstemmed | Modulation of Vasomotive Activity in Rabbit External Ophthalmic
Artery by Neuropeptides |
| title_short | Modulation of Vasomotive Activity in Rabbit External Ophthalmic
Artery by Neuropeptides |
| title_sort | modulation of vasomotive activity in rabbit external ophthalmic artery by neuropeptides |
| url | http://dx.doi.org/10.1155/2012/498565 |
| work_keys_str_mv | AT esmeraldasofiacostadelgado modulationofvasomotiveactivityinrabbitexternalophthalmicarterybyneuropeptides AT carlosmarquesneves modulationofvasomotiveactivityinrabbitexternalophthalmicarterybyneuropeptides AT mariaisabelsousarocha modulationofvasomotiveactivityinrabbitexternalophthalmicarterybyneuropeptides AT josepaulopachecosalesluis modulationofvasomotiveactivityinrabbitexternalophthalmicarterybyneuropeptides AT luisfilipesilvacarvalho modulationofvasomotiveactivityinrabbitexternalophthalmicarterybyneuropeptides |