Rapid Screening and Quantitative Determination of Illegal Phosphodiesterase Type 5 Inhibitors (PDE-5i) in Herbal Dietary Supplements
Phosphodiesterase type 5 inhibitors (PDE-5i) are the first-line medication for oral erectile dysfunction, which are used according to the prescription of doctors. However, these substances have been found illegally in supplementary foods. The quality and safety of dietary supplements for enhancing m...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2021/5579500 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832550597192056832 |
|---|---|
| author | Thi Oanh Nguyen Cao-Son Tran Thi Thu Hang Do Thi Minh Hoa Nguyen Quang-Dong Bui Cao-Tien Bui Hong-Ngoc Nguyen Thu-Hien Dang Viet-Chien Dinh Thi Anh Huong Nguyen Thi Hong Hao Le |
| author_facet | Thi Oanh Nguyen Cao-Son Tran Thi Thu Hang Do Thi Minh Hoa Nguyen Quang-Dong Bui Cao-Tien Bui Hong-Ngoc Nguyen Thu-Hien Dang Viet-Chien Dinh Thi Anh Huong Nguyen Thi Hong Hao Le |
| author_sort | Thi Oanh Nguyen |
| collection | DOAJ |
| description | Phosphodiesterase type 5 inhibitors (PDE-5i) are the first-line medication for oral erectile dysfunction, which are used according to the prescription of doctors. However, these substances have been found illegally in supplementary foods. The quality and safety of dietary supplements for enhancing male sexual performance have been questioned, raising the need for continual development of analytical methods. Liquid chromatography coupled with high-resolution mass spectrometry has become one of the most effective methods to identify and measure PDE-5i concentration. In this research, we focused on (i) developing and validating an effective screening and quantitation method for more than 53 PDE-5i in ingredients and supplementary products using LC-Q-Exactive after a simple sample extraction and (ii) assessing PDE-5i content in natural-based supplementary products available in Vietnam market. The extraction method used a small amount of organic solvent, which makes it more environmentally friendly (greener). The developed method has a limit of detection of 0.4 mg/kg, a limit of quantitation of 1.2 mg/kg, recoveries from 80 to 110%, and repeatability lower than 15%. Ninety-two herbal supplementary foods and ingredients used for enhancement of male sexual performance available in Vietnamese markets were collected. Fourteen PDE-5i including conventional and novel analogous were detected and measured in eighteen food supplements and two formulation ingredient samples. |
| format | Article |
| id | doaj-art-f00ddcf1e190493cbb1f1e7e51159685 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-f00ddcf1e190493cbb1f1e7e511596852025-02-03T06:06:27ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732021-01-01202110.1155/2021/55795005579500Rapid Screening and Quantitative Determination of Illegal Phosphodiesterase Type 5 Inhibitors (PDE-5i) in Herbal Dietary SupplementsThi Oanh Nguyen0Cao-Son Tran1Thi Thu Hang Do2Thi Minh Hoa Nguyen3Quang-Dong Bui4Cao-Tien Bui5Hong-Ngoc Nguyen6Thu-Hien Dang7Viet-Chien Dinh8Thi Anh Huong Nguyen9Thi Hong Hao Le10Vietnam Food Administration, 135 Nui Truc, Ba Dinh, Hanoi 10000, VietnamNational Institute for Food Control, 65 Pham Than Duat, Cau Giay, Hanoi 10000, VietnamNational Institute for Food Control, 65 Pham Than Duat, Cau Giay, Hanoi 10000, VietnamNational Institute for Food Control, 65 Pham Than Duat, Cau Giay, Hanoi 10000, VietnamNational Institute for Food Control, 65 Pham Than Duat, Cau Giay, Hanoi 10000, VietnamNational Institute for Food Control, 65 Pham Than Duat, Cau Giay, Hanoi 10000, VietnamNational Institute for Food Control, 65 Pham Than Duat, Cau Giay, Hanoi 10000, VietnamNational Institute for Food Control, 65 Pham Than Duat, Cau Giay, Hanoi 10000, VietnamNational Institute for Food Control, 65 Pham Than Duat, Cau Giay, Hanoi 10000, VietnamUniversity of Science, Vietnam National University, 334 Nguyen Trai, Thanh Xuan, Hanoi 10000, VietnamUniversity of Science, Vietnam National University, 334 Nguyen Trai, Thanh Xuan, Hanoi 10000, VietnamPhosphodiesterase type 5 inhibitors (PDE-5i) are the first-line medication for oral erectile dysfunction, which are used according to the prescription of doctors. However, these substances have been found illegally in supplementary foods. The quality and safety of dietary supplements for enhancing male sexual performance have been questioned, raising the need for continual development of analytical methods. Liquid chromatography coupled with high-resolution mass spectrometry has become one of the most effective methods to identify and measure PDE-5i concentration. In this research, we focused on (i) developing and validating an effective screening and quantitation method for more than 53 PDE-5i in ingredients and supplementary products using LC-Q-Exactive after a simple sample extraction and (ii) assessing PDE-5i content in natural-based supplementary products available in Vietnam market. The extraction method used a small amount of organic solvent, which makes it more environmentally friendly (greener). The developed method has a limit of detection of 0.4 mg/kg, a limit of quantitation of 1.2 mg/kg, recoveries from 80 to 110%, and repeatability lower than 15%. Ninety-two herbal supplementary foods and ingredients used for enhancement of male sexual performance available in Vietnamese markets were collected. Fourteen PDE-5i including conventional and novel analogous were detected and measured in eighteen food supplements and two formulation ingredient samples.http://dx.doi.org/10.1155/2021/5579500 |
| spellingShingle | Thi Oanh Nguyen Cao-Son Tran Thi Thu Hang Do Thi Minh Hoa Nguyen Quang-Dong Bui Cao-Tien Bui Hong-Ngoc Nguyen Thu-Hien Dang Viet-Chien Dinh Thi Anh Huong Nguyen Thi Hong Hao Le Rapid Screening and Quantitative Determination of Illegal Phosphodiesterase Type 5 Inhibitors (PDE-5i) in Herbal Dietary Supplements Journal of Analytical Methods in Chemistry |
| title | Rapid Screening and Quantitative Determination of Illegal Phosphodiesterase Type 5 Inhibitors (PDE-5i) in Herbal Dietary Supplements |
| title_full | Rapid Screening and Quantitative Determination of Illegal Phosphodiesterase Type 5 Inhibitors (PDE-5i) in Herbal Dietary Supplements |
| title_fullStr | Rapid Screening and Quantitative Determination of Illegal Phosphodiesterase Type 5 Inhibitors (PDE-5i) in Herbal Dietary Supplements |
| title_full_unstemmed | Rapid Screening and Quantitative Determination of Illegal Phosphodiesterase Type 5 Inhibitors (PDE-5i) in Herbal Dietary Supplements |
| title_short | Rapid Screening and Quantitative Determination of Illegal Phosphodiesterase Type 5 Inhibitors (PDE-5i) in Herbal Dietary Supplements |
| title_sort | rapid screening and quantitative determination of illegal phosphodiesterase type 5 inhibitors pde 5i in herbal dietary supplements |
| url | http://dx.doi.org/10.1155/2021/5579500 |
| work_keys_str_mv | AT thioanhnguyen rapidscreeningandquantitativedeterminationofillegalphosphodiesterasetype5inhibitorspde5iinherbaldietarysupplements AT caosontran rapidscreeningandquantitativedeterminationofillegalphosphodiesterasetype5inhibitorspde5iinherbaldietarysupplements AT thithuhangdo rapidscreeningandquantitativedeterminationofillegalphosphodiesterasetype5inhibitorspde5iinherbaldietarysupplements AT thiminhhoanguyen rapidscreeningandquantitativedeterminationofillegalphosphodiesterasetype5inhibitorspde5iinherbaldietarysupplements AT quangdongbui rapidscreeningandquantitativedeterminationofillegalphosphodiesterasetype5inhibitorspde5iinherbaldietarysupplements AT caotienbui rapidscreeningandquantitativedeterminationofillegalphosphodiesterasetype5inhibitorspde5iinherbaldietarysupplements AT hongngocnguyen rapidscreeningandquantitativedeterminationofillegalphosphodiesterasetype5inhibitorspde5iinherbaldietarysupplements AT thuhiendang rapidscreeningandquantitativedeterminationofillegalphosphodiesterasetype5inhibitorspde5iinherbaldietarysupplements AT vietchiendinh rapidscreeningandquantitativedeterminationofillegalphosphodiesterasetype5inhibitorspde5iinherbaldietarysupplements AT thianhhuongnguyen rapidscreeningandquantitativedeterminationofillegalphosphodiesterasetype5inhibitorspde5iinherbaldietarysupplements AT thihonghaole rapidscreeningandquantitativedeterminationofillegalphosphodiesterasetype5inhibitorspde5iinherbaldietarysupplements |