Lutein Leads to a Decrease of Factor D Secretion by Cultured Mature Human Adipocytes
Purpose. Complement plays an important role in the pathogenesis of age related macular degeneration (AMD) and trials are currently being conducted to investigate the effect of complement inhibition on AMD progression. We previously found that the plasma level of factor D (FD), which is the rate limi...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2015/430741 |
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| author | Yuan Tian Aize Kijlstra Johan Renes Martin Wabitsch Carroll A. B. Webers Tos T. J. M. Berendschot |
| author_facet | Yuan Tian Aize Kijlstra Johan Renes Martin Wabitsch Carroll A. B. Webers Tos T. J. M. Berendschot |
| author_sort | Yuan Tian |
| collection | DOAJ |
| description | Purpose. Complement plays an important role in the pathogenesis of age related macular degeneration (AMD) and trials are currently being conducted to investigate the effect of complement inhibition on AMD progression. We previously found that the plasma level of factor D (FD), which is the rate limiting enzyme of the complement alternative pathway, was significantly decreased following lutein supplementation. FD is synthesized by adipose tissue, which is also the main storage site of lutein. In view of these findings we tested the hypothesis whether lutein could affect FD synthesis by adipocytes. Methods. A cell line of mature human adipocytes was incubated with 50 μg/mL lutein for 24 and 48 h, whereafter FD mRNA and protein expression were measured. Results. Lutein significantly inhibited adipocyte FD mRNA expression and FD protein release into adipocyte culture supernatants. Conclusions. Our earlier observations showing that a daily lutein supplement in individuals with early signs of AMD lowered the level of circulating FD might be caused by blocking adipocyte FD production. |
| format | Article |
| id | doaj-art-f00d4f8617df4b2594097fb15d47447b |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-f00d4f8617df4b2594097fb15d47447b2025-02-03T06:08:38ZengWileyJournal of Ophthalmology2090-004X2090-00582015-01-01201510.1155/2015/430741430741Lutein Leads to a Decrease of Factor D Secretion by Cultured Mature Human AdipocytesYuan Tian0Aize Kijlstra1Johan Renes2Martin Wabitsch3Carroll A. B. Webers4Tos T. J. M. Berendschot5University Eye Clinic Maastricht, Postbus 5800, 6202 AZ Maastricht, NetherlandsUniversity Eye Clinic Maastricht, Postbus 5800, 6202 AZ Maastricht, NetherlandsDepartment of Human Biology, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University, Maastricht, NetherlandsSektion Pädiatrische Endokrinologie u. Diabetologie, Universität Ulm, GermanyUniversity Eye Clinic Maastricht, Postbus 5800, 6202 AZ Maastricht, NetherlandsUniversity Eye Clinic Maastricht, Postbus 5800, 6202 AZ Maastricht, NetherlandsPurpose. Complement plays an important role in the pathogenesis of age related macular degeneration (AMD) and trials are currently being conducted to investigate the effect of complement inhibition on AMD progression. We previously found that the plasma level of factor D (FD), which is the rate limiting enzyme of the complement alternative pathway, was significantly decreased following lutein supplementation. FD is synthesized by adipose tissue, which is also the main storage site of lutein. In view of these findings we tested the hypothesis whether lutein could affect FD synthesis by adipocytes. Methods. A cell line of mature human adipocytes was incubated with 50 μg/mL lutein for 24 and 48 h, whereafter FD mRNA and protein expression were measured. Results. Lutein significantly inhibited adipocyte FD mRNA expression and FD protein release into adipocyte culture supernatants. Conclusions. Our earlier observations showing that a daily lutein supplement in individuals with early signs of AMD lowered the level of circulating FD might be caused by blocking adipocyte FD production.http://dx.doi.org/10.1155/2015/430741 |
| spellingShingle | Yuan Tian Aize Kijlstra Johan Renes Martin Wabitsch Carroll A. B. Webers Tos T. J. M. Berendschot Lutein Leads to a Decrease of Factor D Secretion by Cultured Mature Human Adipocytes Journal of Ophthalmology |
| title | Lutein Leads to a Decrease of Factor D Secretion by Cultured Mature Human Adipocytes |
| title_full | Lutein Leads to a Decrease of Factor D Secretion by Cultured Mature Human Adipocytes |
| title_fullStr | Lutein Leads to a Decrease of Factor D Secretion by Cultured Mature Human Adipocytes |
| title_full_unstemmed | Lutein Leads to a Decrease of Factor D Secretion by Cultured Mature Human Adipocytes |
| title_short | Lutein Leads to a Decrease of Factor D Secretion by Cultured Mature Human Adipocytes |
| title_sort | lutein leads to a decrease of factor d secretion by cultured mature human adipocytes |
| url | http://dx.doi.org/10.1155/2015/430741 |
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