Xanthine Oxidase: Isolation, Assays of Activity, and Inhibition
Xanthine oxidase (XO) is an important enzyme catalyzing the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid which is excreted by kidneys. Excessive production and/or inadequate excretion of uric acid results in hyperuricemia. This paper presents a detailed review of methods of is...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/294858 |
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| author | Danijela A. Kostić Danica S. Dimitrijević Gordana S. Stojanović Ivan R. Palić Aleksandra S. Đorđević Jovana D. Ickovski |
| author_facet | Danijela A. Kostić Danica S. Dimitrijević Gordana S. Stojanović Ivan R. Palić Aleksandra S. Đorđević Jovana D. Ickovski |
| author_sort | Danijela A. Kostić |
| collection | DOAJ |
| description | Xanthine oxidase (XO) is an important enzyme catalyzing the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid which is excreted by kidneys. Excessive production and/or inadequate excretion of uric acid results in hyperuricemia. This paper presents a detailed review of methods of isolation, determination of xanthine oxidase activity, and the effect of plant extracts and their constituents on it. Determining the content and activities of XO can be used for diagnostic purposes. Testing inhibition of XO is important for detection of potentially effective compounds or extracts that can be used to treat diseases that are caused by increased activity of XO. In vitro bioassays are used to examine test material for XO inhibition, as inhibitors of XO may be potentially useful for the treatment of gout or other XO induced diseases. Several authors reported on the XO inhibitory potential of traditionally used medicinal plants. |
| format | Article |
| id | doaj-art-f00985630a384e96acd738f9556778c5 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-f00985630a384e96acd738f9556778c52025-02-03T06:08:38ZengWileyJournal of Chemistry2090-90632090-90712015-01-01201510.1155/2015/294858294858Xanthine Oxidase: Isolation, Assays of Activity, and InhibitionDanijela A. Kostić0Danica S. Dimitrijević1Gordana S. Stojanović2Ivan R. Palić3Aleksandra S. Đorđević4Jovana D. Ickovski5Department of Chemistry, Faculty of Science and Mathematics, University of Nis, Visegradska 33, 18 000 Nis, SerbiaDepartment of Chemistry, Faculty of Science and Mathematics, University of Nis, Visegradska 33, 18 000 Nis, SerbiaDepartment of Chemistry, Faculty of Science and Mathematics, University of Nis, Visegradska 33, 18 000 Nis, SerbiaDepartment of Chemistry, Faculty of Science and Mathematics, University of Nis, Visegradska 33, 18 000 Nis, SerbiaDepartment of Chemistry, Faculty of Science and Mathematics, University of Nis, Visegradska 33, 18 000 Nis, SerbiaDepartment of Chemistry, Faculty of Science and Mathematics, University of Nis, Visegradska 33, 18 000 Nis, SerbiaXanthine oxidase (XO) is an important enzyme catalyzing the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid which is excreted by kidneys. Excessive production and/or inadequate excretion of uric acid results in hyperuricemia. This paper presents a detailed review of methods of isolation, determination of xanthine oxidase activity, and the effect of plant extracts and their constituents on it. Determining the content and activities of XO can be used for diagnostic purposes. Testing inhibition of XO is important for detection of potentially effective compounds or extracts that can be used to treat diseases that are caused by increased activity of XO. In vitro bioassays are used to examine test material for XO inhibition, as inhibitors of XO may be potentially useful for the treatment of gout or other XO induced diseases. Several authors reported on the XO inhibitory potential of traditionally used medicinal plants.http://dx.doi.org/10.1155/2015/294858 |
| spellingShingle | Danijela A. Kostić Danica S. Dimitrijević Gordana S. Stojanović Ivan R. Palić Aleksandra S. Đorđević Jovana D. Ickovski Xanthine Oxidase: Isolation, Assays of Activity, and Inhibition Journal of Chemistry |
| title | Xanthine Oxidase: Isolation, Assays of Activity, and Inhibition |
| title_full | Xanthine Oxidase: Isolation, Assays of Activity, and Inhibition |
| title_fullStr | Xanthine Oxidase: Isolation, Assays of Activity, and Inhibition |
| title_full_unstemmed | Xanthine Oxidase: Isolation, Assays of Activity, and Inhibition |
| title_short | Xanthine Oxidase: Isolation, Assays of Activity, and Inhibition |
| title_sort | xanthine oxidase isolation assays of activity and inhibition |
| url | http://dx.doi.org/10.1155/2015/294858 |
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