Immune regulation is more effective in the U937 inflammation model with mesenchymal stem cell extracellular vesicles stimulated by pro-inflammatory cytokines
Mesenchymal stem cells (MSCs), which are multipotent adult cells with many therapeutic effects, can be derived from stromal tissues. MSCs also exert immunoregulatory effects through extracellular vesicles (EVs), cell membrane structures that carry paracrine factors. It is thought that the mediators...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Termedia Publishing House
2024-11-01
|
| Series: | Central European Journal of Immunology |
| Subjects: | |
| Online Access: | https://www.termedia.pl/Immune-regulation-is-more-effective-in-the-U937-inflammation-model-with-mesenchymal-stem-cell-extracellular-vesicles-stimulated-by-pro-inflammatory-cytokines,10,54944,1,1.html |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832584830774149120 |
|---|---|
| author | Canan Öztürk Zehra S. Halbutoğullari |
| author_facet | Canan Öztürk Zehra S. Halbutoğullari |
| author_sort | Canan Öztürk |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs), which are multipotent adult cells with many therapeutic effects, can be derived from stromal tissues. MSCs also exert immunoregulatory effects through extracellular vesicles (EVs), cell membrane structures that carry paracrine factors. It is thought that the mediators (cytokines, growth factors, etc.) secreted by stem cells change under inflammatory conditions, and the therapeutic activity of MSCs increases. The purpose of this study was to investigate the possible effects of stimulated human Wharton’s jelly-derived mesenchymal stem cell extracellular vesicles, obtained with or without stimulation with inflammatory cytokines, on inflammation. The study aimed to determine the effects of pro-inflammatory cytokines interleukin 1β (IL-1β), interferon γ (IFN-γ), and tumor necrosis factor α (TNF-α) stimulated extracellular vesicles (sEVs) on the inflammation model U937 macrophages induced by phorbol-12-myristate 13-acetate (PMA) and lipopolysaccharide (LPS) treatment.
Experimental studies were designed to investigate the effects of EVs obtained without stimulation with inflammatory cytokines and those obtained after stimulation with inflammatory cytokines in the macrophage cell line U937. Flow cytometry, gene expression, and immunofluorescence analyses were performed to investigate the apoptotic and antiproliferative effects of EVs and sEVs in the U937 macrophage inflammation model.
WJ-MSC EVs obtained after culture with inflammatory cytokines had a greater apoptotic effect on U937 cells and reduced inflammatory cytokine release than EVs cultured in standard medium. |
| format | Article |
| id | doaj-art-f0075ed97f6044f38733cae694c550b0 |
| institution | Kabale University |
| issn | 1426-3912 1644-4124 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Central European Journal of Immunology |
| spelling | doaj-art-f0075ed97f6044f38733cae694c550b02025-01-27T11:13:18ZengTermedia Publishing HouseCentral European Journal of Immunology1426-39121644-41242024-11-0149328229910.5114/ceji.2024.14372654944Immune regulation is more effective in the U937 inflammation model with mesenchymal stem cell extracellular vesicles stimulated by pro-inflammatory cytokinesCanan ÖztürkZehra S. HalbutoğullariMesenchymal stem cells (MSCs), which are multipotent adult cells with many therapeutic effects, can be derived from stromal tissues. MSCs also exert immunoregulatory effects through extracellular vesicles (EVs), cell membrane structures that carry paracrine factors. It is thought that the mediators (cytokines, growth factors, etc.) secreted by stem cells change under inflammatory conditions, and the therapeutic activity of MSCs increases. The purpose of this study was to investigate the possible effects of stimulated human Wharton’s jelly-derived mesenchymal stem cell extracellular vesicles, obtained with or without stimulation with inflammatory cytokines, on inflammation. The study aimed to determine the effects of pro-inflammatory cytokines interleukin 1β (IL-1β), interferon γ (IFN-γ), and tumor necrosis factor α (TNF-α) stimulated extracellular vesicles (sEVs) on the inflammation model U937 macrophages induced by phorbol-12-myristate 13-acetate (PMA) and lipopolysaccharide (LPS) treatment. Experimental studies were designed to investigate the effects of EVs obtained without stimulation with inflammatory cytokines and those obtained after stimulation with inflammatory cytokines in the macrophage cell line U937. Flow cytometry, gene expression, and immunofluorescence analyses were performed to investigate the apoptotic and antiproliferative effects of EVs and sEVs in the U937 macrophage inflammation model. WJ-MSC EVs obtained after culture with inflammatory cytokines had a greater apoptotic effect on U937 cells and reduced inflammatory cytokine release than EVs cultured in standard medium.https://www.termedia.pl/Immune-regulation-is-more-effective-in-the-U937-inflammation-model-with-mesenchymal-stem-cell-extracellular-vesicles-stimulated-by-pro-inflammatory-cytokines,10,54944,1,1.htmlu937 macrophage lipopolysaccharide (lps) wharton’s jelly mesenchymal stem cells (wj-mscs) extracellular vesicles (evs) |
| spellingShingle | Canan Öztürk Zehra S. Halbutoğullari Immune regulation is more effective in the U937 inflammation model with mesenchymal stem cell extracellular vesicles stimulated by pro-inflammatory cytokines Central European Journal of Immunology u937 macrophage lipopolysaccharide (lps) wharton’s jelly mesenchymal stem cells (wj-mscs) extracellular vesicles (evs) |
| title | Immune regulation is more effective in the U937 inflammation model with mesenchymal stem cell extracellular vesicles stimulated by pro-inflammatory cytokines |
| title_full | Immune regulation is more effective in the U937 inflammation model with mesenchymal stem cell extracellular vesicles stimulated by pro-inflammatory cytokines |
| title_fullStr | Immune regulation is more effective in the U937 inflammation model with mesenchymal stem cell extracellular vesicles stimulated by pro-inflammatory cytokines |
| title_full_unstemmed | Immune regulation is more effective in the U937 inflammation model with mesenchymal stem cell extracellular vesicles stimulated by pro-inflammatory cytokines |
| title_short | Immune regulation is more effective in the U937 inflammation model with mesenchymal stem cell extracellular vesicles stimulated by pro-inflammatory cytokines |
| title_sort | immune regulation is more effective in the u937 inflammation model with mesenchymal stem cell extracellular vesicles stimulated by pro inflammatory cytokines |
| topic | u937 macrophage lipopolysaccharide (lps) wharton’s jelly mesenchymal stem cells (wj-mscs) extracellular vesicles (evs) |
| url | https://www.termedia.pl/Immune-regulation-is-more-effective-in-the-U937-inflammation-model-with-mesenchymal-stem-cell-extracellular-vesicles-stimulated-by-pro-inflammatory-cytokines,10,54944,1,1.html |
| work_keys_str_mv | AT cananozturk immuneregulationismoreeffectiveintheu937inflammationmodelwithmesenchymalstemcellextracellularvesiclesstimulatedbyproinflammatorycytokines AT zehrashalbutogullari immuneregulationismoreeffectiveintheu937inflammationmodelwithmesenchymalstemcellextracellularvesiclesstimulatedbyproinflammatorycytokines |