A Role of Fluoride on Free Radical Generation and Oxidative Stress in BV-2 Microglia Cells
The generation of ROS and lipid peroxidation has been considered to play an important role in the pathogenesis of chronic fluoride toxicity. In the present study, we observed that fluoride activated BV-2 microglia cell line by observing OX-42 expression in immunocytochemistry. Intracellular superoxi...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/102954 |
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| author | Xi Shuhua Liu Ziyou Yan Ling Wang Fei Guifan Sun |
| author_facet | Xi Shuhua Liu Ziyou Yan Ling Wang Fei Guifan Sun |
| author_sort | Xi Shuhua |
| collection | DOAJ |
| description | The generation of ROS and lipid peroxidation has been considered to play an important role in the pathogenesis of chronic fluoride toxicity. In the present study, we observed that fluoride activated BV-2 microglia cell line by observing OX-42 expression in immunocytochemistry. Intracellular superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), reactive oxygen species (ROS), superoxide anions (O2∙-), nitric oxide synthase (NOS), nitrotyrosine (NT) and nitric oxide (NO), NOS in cell medium were determined for oxidative stress assessment. Our study found that NaF of concentration from 5 to 20 mg/L can stimuli BV-2 cells to change into activated microglia displaying upregulated OX-42 expression. SOD activities significantly decreased in fluoride-treated BV-2 cells as compared with control, and MDA concentrations and contents of ROS and O2∙- increased in NaF-treated cells. Activities of NOS in cells and medium significantly increased with fluoride concentrations in a dose-dependent manner. NT concentrations also increased significantly in 10 and 50 mg/L NaF-treated cells compared with the control cells. Our present study demonstrated that toxic effects of fluoride on the central nervous system possibly partly ascribed to activiting of microglia, which enhanced oxidative stress induced by ROS and reactive nitrogen species. |
| format | Article |
| id | doaj-art-efed5cfe6db9491db025e8e4ca119ebd |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-efed5cfe6db9491db025e8e4ca119ebd2025-02-03T01:10:34ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/102954102954A Role of Fluoride on Free Radical Generation and Oxidative Stress in BV-2 Microglia CellsXi Shuhua0Liu Ziyou1Yan Ling2Wang Fei3Guifan Sun4Department of Environmental and Occupational Health, Liaoning Provincial Key Laboratory of Arsenic Biological Effect and Poisoning, School of Public Health, China Medical University, District of Heping, North Er Road No. 92, Shenyang 110001, ChinaDepartment of Environmental and Occupational Health, Liaoning Provincial Key Laboratory of Arsenic Biological Effect and Poisoning, School of Public Health, China Medical University, District of Heping, North Er Road No. 92, Shenyang 110001, ChinaDepartment of Environmental and Occupational Health, Liaoning Provincial Key Laboratory of Arsenic Biological Effect and Poisoning, School of Public Health, China Medical University, District of Heping, North Er Road No. 92, Shenyang 110001, ChinaDepartment of Environmental and Occupational Health, Liaoning Provincial Key Laboratory of Arsenic Biological Effect and Poisoning, School of Public Health, China Medical University, District of Heping, North Er Road No. 92, Shenyang 110001, ChinaDepartment of Environmental and Occupational Health, Liaoning Provincial Key Laboratory of Arsenic Biological Effect and Poisoning, School of Public Health, China Medical University, District of Heping, North Er Road No. 92, Shenyang 110001, ChinaThe generation of ROS and lipid peroxidation has been considered to play an important role in the pathogenesis of chronic fluoride toxicity. In the present study, we observed that fluoride activated BV-2 microglia cell line by observing OX-42 expression in immunocytochemistry. Intracellular superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), reactive oxygen species (ROS), superoxide anions (O2∙-), nitric oxide synthase (NOS), nitrotyrosine (NT) and nitric oxide (NO), NOS in cell medium were determined for oxidative stress assessment. Our study found that NaF of concentration from 5 to 20 mg/L can stimuli BV-2 cells to change into activated microglia displaying upregulated OX-42 expression. SOD activities significantly decreased in fluoride-treated BV-2 cells as compared with control, and MDA concentrations and contents of ROS and O2∙- increased in NaF-treated cells. Activities of NOS in cells and medium significantly increased with fluoride concentrations in a dose-dependent manner. NT concentrations also increased significantly in 10 and 50 mg/L NaF-treated cells compared with the control cells. Our present study demonstrated that toxic effects of fluoride on the central nervous system possibly partly ascribed to activiting of microglia, which enhanced oxidative stress induced by ROS and reactive nitrogen species.http://dx.doi.org/10.1155/2012/102954 |
| spellingShingle | Xi Shuhua Liu Ziyou Yan Ling Wang Fei Guifan Sun A Role of Fluoride on Free Radical Generation and Oxidative Stress in BV-2 Microglia Cells Mediators of Inflammation |
| title | A Role of Fluoride on Free Radical Generation and Oxidative Stress in BV-2 Microglia Cells |
| title_full | A Role of Fluoride on Free Radical Generation and Oxidative Stress in BV-2 Microglia Cells |
| title_fullStr | A Role of Fluoride on Free Radical Generation and Oxidative Stress in BV-2 Microglia Cells |
| title_full_unstemmed | A Role of Fluoride on Free Radical Generation and Oxidative Stress in BV-2 Microglia Cells |
| title_short | A Role of Fluoride on Free Radical Generation and Oxidative Stress in BV-2 Microglia Cells |
| title_sort | role of fluoride on free radical generation and oxidative stress in bv 2 microglia cells |
| url | http://dx.doi.org/10.1155/2012/102954 |
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