Network pharmacology-based screening of the active ingredients and mechanisms of Cymbaria daurica against diabetes mellitus
Cymbaria daurica L. has a long history as a folk medicine and tea for the treatment of diabetes. However, the biological activity and mechanism of its hypoglycemic effect have not been fully elucidated. In this study, the potential mechanism of C. daurica against type 2 diabetes mellitus (T2DM) was...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000745 |
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| author | Ruyu Shi Dongxue Chen Mingyue Ji Baochang Zhou Ziyan Zhang Chunhong Zhang Minhui Li |
| author_facet | Ruyu Shi Dongxue Chen Mingyue Ji Baochang Zhou Ziyan Zhang Chunhong Zhang Minhui Li |
| author_sort | Ruyu Shi |
| collection | DOAJ |
| description | Cymbaria daurica L. has a long history as a folk medicine and tea for the treatment of diabetes. However, the biological activity and mechanism of its hypoglycemic effect have not been fully elucidated. In this study, the potential mechanism of C. daurica against type 2 diabetes mellitus (T2DM) was postulated via pharmacological network analysis. Based on data mining techniques involving topological parameters, gene ontology, and pathway enrichment, we established a compound-target, protein-protein interaction, and target-pathway network to identify central targets and pathways. Pathway enrichment analysis revealed that the most important pathway associated with C. daurica in treating T2DM is the PI3K-Akt signaling pathway. Molecular docking was performed to validate the predicted results. Then, a HepG2 cell insulin resistance model and a high-fat, high-glucose diet combined with a streptozotocin-induced T2DM rat model was established to assess the fasting glucose changes and lipid profile after C. daurica treatment, respectively. Finally, real-time PCR and western blotting were used to verify changes in key targets. The anti-diabetic mechanism of the active ingredient in C. daurica may involve the regulation of IRS-2, Akt1, GLUT4, and GSK3β. |
| format | Article |
| id | doaj-art-efebbe73677b4a428b4e16241a6d6cde |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-efebbe73677b4a428b4e16241a6d6cde2025-02-03T02:42:14ZengTsinghua University PressFood Science and Human Wellness2213-45302023-11-0112620012013Network pharmacology-based screening of the active ingredients and mechanisms of Cymbaria daurica against diabetes mellitusRuyu Shi0Dongxue Chen1Mingyue Ji2Baochang Zhou3Ziyan Zhang4Chunhong Zhang5Minhui Li6Inner Mongolia Hospital of Traditional Chinese Medicine, Hohhot 010020, China; Inner Mongolia Key Laboratory of Characteristic Geoherbs Resources Protection and Utilization, Baotou Medical College, Baotou 014040, ChinaInner Mongolia Hospital of Traditional Chinese Medicine, Hohhot 010020, China; Inner Mongolia Institute of Traditional Chinese Medicine, Hohhot 010020, ChinaInner Mongolia Key Laboratory of Characteristic Geoherbs Resources Protection and Utilization, Baotou Medical College, Baotou 014040, China; Inner Mongolia Engineering Research Center of the Planting and Development of Astragalus membranaceus of the Geoherbs, Baotou Medical College, Baotou 014040, ChinaInner Mongolia Hospital of Traditional Chinese Medicine, Hohhot 010020, China; Inner Mongolia Institute of Traditional Chinese Medicine, Hohhot 010020, ChinaInner Mongolia Hospital of Traditional Chinese Medicine, Hohhot 010020, China; Inner Mongolia Institute of Traditional Chinese Medicine, Hohhot 010020, ChinaInner Mongolia Engineering Research Center of the Planting and Development of Astragalus membranaceus of the Geoherbs, Baotou Medical College, Baotou 014040, China; Inner Mongolia Key Laboratory of Chinese Medicine Resources, Baotou Medical College, Baotou 014040, China; Corresponding authors.Inner Mongolia Hospital of Traditional Chinese Medicine, Hohhot 010020, China; Inner Mongolia Key Laboratory of Characteristic Geoherbs Resources Protection and Utilization, Baotou Medical College, Baotou 014040, China; Inner Mongolia Institute of Traditional Chinese Medicine, Hohhot 010020, China; Corresponding authors.Cymbaria daurica L. has a long history as a folk medicine and tea for the treatment of diabetes. However, the biological activity and mechanism of its hypoglycemic effect have not been fully elucidated. In this study, the potential mechanism of C. daurica against type 2 diabetes mellitus (T2DM) was postulated via pharmacological network analysis. Based on data mining techniques involving topological parameters, gene ontology, and pathway enrichment, we established a compound-target, protein-protein interaction, and target-pathway network to identify central targets and pathways. Pathway enrichment analysis revealed that the most important pathway associated with C. daurica in treating T2DM is the PI3K-Akt signaling pathway. Molecular docking was performed to validate the predicted results. Then, a HepG2 cell insulin resistance model and a high-fat, high-glucose diet combined with a streptozotocin-induced T2DM rat model was established to assess the fasting glucose changes and lipid profile after C. daurica treatment, respectively. Finally, real-time PCR and western blotting were used to verify changes in key targets. The anti-diabetic mechanism of the active ingredient in C. daurica may involve the regulation of IRS-2, Akt1, GLUT4, and GSK3β.http://www.sciencedirect.com/science/article/pii/S2213453023000745Cymbaria daurica L.Diabetes mellitusNetwork pharmacologyInsulin resistanceMolecular dockingPI3K-Akt signaling pathway |
| spellingShingle | Ruyu Shi Dongxue Chen Mingyue Ji Baochang Zhou Ziyan Zhang Chunhong Zhang Minhui Li Network pharmacology-based screening of the active ingredients and mechanisms of Cymbaria daurica against diabetes mellitus Food Science and Human Wellness Cymbaria daurica L. Diabetes mellitus Network pharmacology Insulin resistance Molecular docking PI3K-Akt signaling pathway |
| title | Network pharmacology-based screening of the active ingredients and mechanisms of Cymbaria daurica against diabetes mellitus |
| title_full | Network pharmacology-based screening of the active ingredients and mechanisms of Cymbaria daurica against diabetes mellitus |
| title_fullStr | Network pharmacology-based screening of the active ingredients and mechanisms of Cymbaria daurica against diabetes mellitus |
| title_full_unstemmed | Network pharmacology-based screening of the active ingredients and mechanisms of Cymbaria daurica against diabetes mellitus |
| title_short | Network pharmacology-based screening of the active ingredients and mechanisms of Cymbaria daurica against diabetes mellitus |
| title_sort | network pharmacology based screening of the active ingredients and mechanisms of cymbaria daurica against diabetes mellitus |
| topic | Cymbaria daurica L. Diabetes mellitus Network pharmacology Insulin resistance Molecular docking PI3K-Akt signaling pathway |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000745 |
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