Preparation, characterisation, and in vitro cancer‐suppression function of RNA nanoparticles carrying miR‐301b‐3p Inhibitor
Abstract Background Multidrug resistance is the biggest barrier on the way to chemotherapy for lung adenocarcinoma (LUAD). For some LUAD patients with cisplatin (DDP) resistance and poor prognoses, the authors put forward RNA nanoparticles (NPs) carrying miR‐301b‐3p Inhibitor. Methods The NPs were c...
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Wiley
2023-05-01
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| Series: | IET Nanobiotechnology |
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| Online Access: | https://doi.org/10.1049/nbt2.12120 |
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| author | Junjie Zhao Niu Niu Fan Yang Haitao Liu Weibo Qi |
| author_facet | Junjie Zhao Niu Niu Fan Yang Haitao Liu Weibo Qi |
| author_sort | Junjie Zhao |
| collection | DOAJ |
| description | Abstract Background Multidrug resistance is the biggest barrier on the way to chemotherapy for lung adenocarcinoma (LUAD). For some LUAD patients with cisplatin (DDP) resistance and poor prognoses, the authors put forward RNA nanoparticles (NPs) carrying miR‐301b‐3p Inhibitor. Methods The NPs were composed of miR‐301b‐3p, A549 aptamer (A549apt), and Cyanine 5 in a bottom‐up manner with a 3‐way‐junction (3WJ) structure. Diameter, assembly process, and morphology of NPs were observed by Dynamic Light Scattering, Native‐Polyacrylamide Gel Electrophoresis, and Atomic Force Microscopy. Cell internalisation, toxicity, proliferation, migration, invasion, and apoptosis were assayed by confocal laser scanning microscope, CCK8, colony formation assay, Transwell, western blot, and flow cytometry. Results 3WJ‐apt‐miR was evenly distributed, with diameter of 19.61 ± 0.49 nm and triangular branching structures. The accurate delivery of this NP in vivo was ensured by A549 aptamer featuring specific targeting, with smaller side effects than traditional chemotherapy. Such nanomaterials were effectively internalized by cancer cells, with normal cell activity intact. Cancer cell proliferation, invasion, and migration were suppressed, and DDP sensitivity was enhanced, causing DNA damage and facilitating apoptosis of DDP‐resistant cells. Conclusion Based on RNA self‐assembling, the authors researched the effect of miRNA on DDP sensitivity in LUAD regarding gene regulation. 3WJ‐apt‐miR paves the way for clinical tumour therapy. |
| format | Article |
| id | doaj-art-efe2f951c4014b5a8c82937f143cec00 |
| institution | Kabale University |
| issn | 1751-8741 1751-875X |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Wiley |
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| series | IET Nanobiotechnology |
| spelling | doaj-art-efe2f951c4014b5a8c82937f143cec002025-02-03T06:45:07ZengWileyIET Nanobiotechnology1751-87411751-875X2023-05-0117322423310.1049/nbt2.12120Preparation, characterisation, and in vitro cancer‐suppression function of RNA nanoparticles carrying miR‐301b‐3p InhibitorJunjie Zhao0Niu Niu1Fan Yang2Haitao Liu3Weibo Qi4Department of Cardiothoracic Surgery Affiliated Hospital of Jiaxing University (the First Hospital of Jiaxing) Jiaxing Zhejiang ChinaDepartment of Cardiothoracic Surgery Affiliated Hospital of Jiaxing University (the First Hospital of Jiaxing) Jiaxing Zhejiang ChinaDepartment of Cardiothoracic Surgery Affiliated Hospital of Jiaxing University (the First Hospital of Jiaxing) Jiaxing Zhejiang ChinaDepartment of Cardiothoracic Surgery Affiliated Hospital of Jiaxing University (the First Hospital of Jiaxing) Jiaxing Zhejiang ChinaDepartment of Cardiothoracic Surgery Affiliated Hospital of Jiaxing University (the First Hospital of Jiaxing) Jiaxing Zhejiang ChinaAbstract Background Multidrug resistance is the biggest barrier on the way to chemotherapy for lung adenocarcinoma (LUAD). For some LUAD patients with cisplatin (DDP) resistance and poor prognoses, the authors put forward RNA nanoparticles (NPs) carrying miR‐301b‐3p Inhibitor. Methods The NPs were composed of miR‐301b‐3p, A549 aptamer (A549apt), and Cyanine 5 in a bottom‐up manner with a 3‐way‐junction (3WJ) structure. Diameter, assembly process, and morphology of NPs were observed by Dynamic Light Scattering, Native‐Polyacrylamide Gel Electrophoresis, and Atomic Force Microscopy. Cell internalisation, toxicity, proliferation, migration, invasion, and apoptosis were assayed by confocal laser scanning microscope, CCK8, colony formation assay, Transwell, western blot, and flow cytometry. Results 3WJ‐apt‐miR was evenly distributed, with diameter of 19.61 ± 0.49 nm and triangular branching structures. The accurate delivery of this NP in vivo was ensured by A549 aptamer featuring specific targeting, with smaller side effects than traditional chemotherapy. Such nanomaterials were effectively internalized by cancer cells, with normal cell activity intact. Cancer cell proliferation, invasion, and migration were suppressed, and DDP sensitivity was enhanced, causing DNA damage and facilitating apoptosis of DDP‐resistant cells. Conclusion Based on RNA self‐assembling, the authors researched the effect of miRNA on DDP sensitivity in LUAD regarding gene regulation. 3WJ‐apt‐miR paves the way for clinical tumour therapy.https://doi.org/10.1049/nbt2.12120nanomedicinetumours |
| spellingShingle | Junjie Zhao Niu Niu Fan Yang Haitao Liu Weibo Qi Preparation, characterisation, and in vitro cancer‐suppression function of RNA nanoparticles carrying miR‐301b‐3p Inhibitor IET Nanobiotechnology nanomedicine tumours |
| title | Preparation, characterisation, and in vitro cancer‐suppression function of RNA nanoparticles carrying miR‐301b‐3p Inhibitor |
| title_full | Preparation, characterisation, and in vitro cancer‐suppression function of RNA nanoparticles carrying miR‐301b‐3p Inhibitor |
| title_fullStr | Preparation, characterisation, and in vitro cancer‐suppression function of RNA nanoparticles carrying miR‐301b‐3p Inhibitor |
| title_full_unstemmed | Preparation, characterisation, and in vitro cancer‐suppression function of RNA nanoparticles carrying miR‐301b‐3p Inhibitor |
| title_short | Preparation, characterisation, and in vitro cancer‐suppression function of RNA nanoparticles carrying miR‐301b‐3p Inhibitor |
| title_sort | preparation characterisation and in vitro cancer suppression function of rna nanoparticles carrying mir 301b 3p inhibitor |
| topic | nanomedicine tumours |
| url | https://doi.org/10.1049/nbt2.12120 |
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