Rapid and noninvasive assessment of adductor muscle performance through shell-closing strength of Zhikong scallop Chlamys farreri
Shell-closing strength (SCS) is a crucial trait of adductor muscle closely associated with the bivalve's swimming ability and health status. However, the investigation and practical application of SCS have been seriously hampered due to the limited availability and complexity of current measure...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235251342400677X |
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| author | Xiangyi Zeng Zujing Yang Mingyi Sui Zhi Liu Qiang Xing Liping Hu Liming Jiang Zhenmin Bao Xiaoting Huang |
| author_facet | Xiangyi Zeng Zujing Yang Mingyi Sui Zhi Liu Qiang Xing Liping Hu Liming Jiang Zhenmin Bao Xiaoting Huang |
| author_sort | Xiangyi Zeng |
| collection | DOAJ |
| description | Shell-closing strength (SCS) is a crucial trait of adductor muscle closely associated with the bivalve's swimming ability and health status. However, the investigation and practical application of SCS have been seriously hampered due to the limited availability and complexity of current measurement methodologies. In this study, a rapid and noninvasive method was developed to quantify SCS with four indicators including total force (TF), time of shell closing (TSC), average force (AF), and maximum force (MF) using a self-developed force gauge device. The optimal measurement time and distance for SCS were three minutes and 0.75 cm, respectively. For ten SCS tests, the coefficients of variation (CV) for all four indicators were between 2.54 % and 5.57 %, and the intraclass correlation coefficients (ICC) exceeded 0.75 in each case, which proved that the measurement results were stable and reliable. Subsequent analysis revealed that the four SCS indicators of scallops were significantly associated with the adductor muscle weight (AMW) and the adductor muscle glycogen content (AMG). The correlation coefficients with AMW were 0.609, 0.258, 0.586, and 0.467 for TF, TSC, AF, and MF, respectively (P < 0.05). For AMG, the correlation coefficients were 0.586, 0.372, 0.506, and 0.334 for TF, TSC, AF, and MF, respectively (P < 0.01). The SCS indicators were then used in a ridge regression multivariate linear model to estimate the contents of AMW and AMG in scallops, yielding coefficients of determination (R2) of 0.81 and 0.87, respectively. Furthermore, scallops with stronger SCS were proved to have longer survival times under heat stress, with correlation coefficients ranging from 0.455 to 0.619 (P < 0.01). This finding indicated that SCS could serve as a reliable metric for assessing the heat resistance of scallops. In conclusion, this study provides a quick, accurate, and noninvasive method for measuring the SCS, which could be useful for the evaluation of adductor muscle performance and the selection breeding of scallops for enhanced quality and resistance traits. |
| format | Article |
| id | doaj-art-efe2788d155044b886fab424770bc1a4 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-efe2788d155044b886fab424770bc1a42025-02-06T05:12:14ZengElsevierAquaculture Reports2352-51342025-03-0140102589Rapid and noninvasive assessment of adductor muscle performance through shell-closing strength of Zhikong scallop Chlamys farreriXiangyi Zeng0Zujing Yang1Mingyi Sui2Zhi Liu3Qiang Xing4Liping Hu5Liming Jiang6Zhenmin Bao7Xiaoting Huang8MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, ChinaMOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, ChinaMOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, ChinaMOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, ChinaMOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao, Shandong 266237, ChinaYantai Marine Economic Research Institute, Yantai, ChinaYantai Marine Economic Research Institute, Yantai, ChinaMOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao, Shandong 266237, China; Laboratory of Tropical Marine Germplasm Resources and Breeding Engineering, Sanya Oceanographic Institution, Ocean University of China, Sanya 572000, ChinaMOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao, Shandong 266237, China; Corresponding author at: MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China.Shell-closing strength (SCS) is a crucial trait of adductor muscle closely associated with the bivalve's swimming ability and health status. However, the investigation and practical application of SCS have been seriously hampered due to the limited availability and complexity of current measurement methodologies. In this study, a rapid and noninvasive method was developed to quantify SCS with four indicators including total force (TF), time of shell closing (TSC), average force (AF), and maximum force (MF) using a self-developed force gauge device. The optimal measurement time and distance for SCS were three minutes and 0.75 cm, respectively. For ten SCS tests, the coefficients of variation (CV) for all four indicators were between 2.54 % and 5.57 %, and the intraclass correlation coefficients (ICC) exceeded 0.75 in each case, which proved that the measurement results were stable and reliable. Subsequent analysis revealed that the four SCS indicators of scallops were significantly associated with the adductor muscle weight (AMW) and the adductor muscle glycogen content (AMG). The correlation coefficients with AMW were 0.609, 0.258, 0.586, and 0.467 for TF, TSC, AF, and MF, respectively (P < 0.05). For AMG, the correlation coefficients were 0.586, 0.372, 0.506, and 0.334 for TF, TSC, AF, and MF, respectively (P < 0.01). The SCS indicators were then used in a ridge regression multivariate linear model to estimate the contents of AMW and AMG in scallops, yielding coefficients of determination (R2) of 0.81 and 0.87, respectively. Furthermore, scallops with stronger SCS were proved to have longer survival times under heat stress, with correlation coefficients ranging from 0.455 to 0.619 (P < 0.01). This finding indicated that SCS could serve as a reliable metric for assessing the heat resistance of scallops. In conclusion, this study provides a quick, accurate, and noninvasive method for measuring the SCS, which could be useful for the evaluation of adductor muscle performance and the selection breeding of scallops for enhanced quality and resistance traits.http://www.sciencedirect.com/science/article/pii/S235251342400677XChlamys farreriShell-closing strengthAdductor muscleGlycogen contentHeat tolerance |
| spellingShingle | Xiangyi Zeng Zujing Yang Mingyi Sui Zhi Liu Qiang Xing Liping Hu Liming Jiang Zhenmin Bao Xiaoting Huang Rapid and noninvasive assessment of adductor muscle performance through shell-closing strength of Zhikong scallop Chlamys farreri Aquaculture Reports Chlamys farreri Shell-closing strength Adductor muscle Glycogen content Heat tolerance |
| title | Rapid and noninvasive assessment of adductor muscle performance through shell-closing strength of Zhikong scallop Chlamys farreri |
| title_full | Rapid and noninvasive assessment of adductor muscle performance through shell-closing strength of Zhikong scallop Chlamys farreri |
| title_fullStr | Rapid and noninvasive assessment of adductor muscle performance through shell-closing strength of Zhikong scallop Chlamys farreri |
| title_full_unstemmed | Rapid and noninvasive assessment of adductor muscle performance through shell-closing strength of Zhikong scallop Chlamys farreri |
| title_short | Rapid and noninvasive assessment of adductor muscle performance through shell-closing strength of Zhikong scallop Chlamys farreri |
| title_sort | rapid and noninvasive assessment of adductor muscle performance through shell closing strength of zhikong scallop chlamys farreri |
| topic | Chlamys farreri Shell-closing strength Adductor muscle Glycogen content Heat tolerance |
| url | http://www.sciencedirect.com/science/article/pii/S235251342400677X |
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