Conversion of Bacterial Gene Products to Secretion-Competent Fusion Proteins
We describe an efficient and easy procedure that allows the generation, detection and secretion of foreign proteins by the secretion apparatus of E. coli hemolysin. The gene (or gene fragment) encoding the foreign protein (or protein domain) is inserted in-frame into a residual portion of the hemoly...
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| Main Authors: | H.-J. Mollenkopf, Ivaylo Gentschev, Werner Goebel |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-11-01
|
| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96215st03 |
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