Conversion of Bacterial Gene Products to Secretion-Competent Fusion Proteins

We describe an efficient and easy procedure that allows the generation, detection and secretion of foreign proteins by the secretion apparatus of E. coli hemolysin. The gene (or gene fragment) encoding the foreign protein (or protein domain) is inserted in-frame into a residual portion of the hemoly...

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Bibliographic Details
Main Authors: H.-J. Mollenkopf, Ivaylo Gentschev, Werner Goebel
Format: Article
Language:English
Published: Taylor & Francis Group 1996-11-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/96215st03
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