Determination of Saponin Content in Hang Maidong and Chuan Maidong via HPLC-ELSD Analysis
Zhejiang and Sichuan are regarded as two genuine producing areas of Ophiopogonis radix in China. To study the difference in the quality of Ophiopogonis radix from these two places, the contents of three reported bioactive saponins, that is, ophiopogonins B, D, and D′, in tubers and fibrous roots of...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2016/7214607 |
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| author | Xian-En Li Yu-Xia Wang Peng Sun Deng-Qun Liao |
| author_facet | Xian-En Li Yu-Xia Wang Peng Sun Deng-Qun Liao |
| author_sort | Xian-En Li |
| collection | DOAJ |
| description | Zhejiang and Sichuan are regarded as two genuine producing areas of Ophiopogonis radix in China. To study the difference in the quality of Ophiopogonis radix from these two places, the contents of three reported bioactive saponins, that is, ophiopogonins B, D, and D′, in tubers and fibrous roots of Ophiopogon japonicus from Cixi city of Zhejiang and Santai county of Sichuan were quantified using HPLC-ELSD method and compared. Ophiopogonin B and ophiopogonin D′ content in tubers of HMD were higher than those in radix of CMD, whereas ophiopogonin D in HMD was about twice lower than that of CMD. Three ophiopogonins were all detected in fibrous roots of both HMD and CMD. Their averaged content in fibrous roots of CMD was about twice higher than that in tubers of CMD. Ophiopogonin D in fibrous roots of CMD was about five times higher than that of HMD. Our biochemical assay revealed that fibrous roots and tubers of CMD can be of an important saponin source, especially for ophiopogonin D. |
| format | Article |
| id | doaj-art-efd8995e5ba2433abf3623fb93180975 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-efd8995e5ba2433abf3623fb931809752025-02-03T05:52:13ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732016-01-01201610.1155/2016/72146077214607Determination of Saponin Content in Hang Maidong and Chuan Maidong via HPLC-ELSD AnalysisXian-En Li0Yu-Xia Wang1Peng Sun2Deng-Qun Liao3Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, ChinaZhejiang and Sichuan are regarded as two genuine producing areas of Ophiopogonis radix in China. To study the difference in the quality of Ophiopogonis radix from these two places, the contents of three reported bioactive saponins, that is, ophiopogonins B, D, and D′, in tubers and fibrous roots of Ophiopogon japonicus from Cixi city of Zhejiang and Santai county of Sichuan were quantified using HPLC-ELSD method and compared. Ophiopogonin B and ophiopogonin D′ content in tubers of HMD were higher than those in radix of CMD, whereas ophiopogonin D in HMD was about twice lower than that of CMD. Three ophiopogonins were all detected in fibrous roots of both HMD and CMD. Their averaged content in fibrous roots of CMD was about twice higher than that in tubers of CMD. Ophiopogonin D in fibrous roots of CMD was about five times higher than that of HMD. Our biochemical assay revealed that fibrous roots and tubers of CMD can be of an important saponin source, especially for ophiopogonin D.http://dx.doi.org/10.1155/2016/7214607 |
| spellingShingle | Xian-En Li Yu-Xia Wang Peng Sun Deng-Qun Liao Determination of Saponin Content in Hang Maidong and Chuan Maidong via HPLC-ELSD Analysis Journal of Analytical Methods in Chemistry |
| title | Determination of Saponin Content in Hang Maidong and Chuan Maidong via HPLC-ELSD Analysis |
| title_full | Determination of Saponin Content in Hang Maidong and Chuan Maidong via HPLC-ELSD Analysis |
| title_fullStr | Determination of Saponin Content in Hang Maidong and Chuan Maidong via HPLC-ELSD Analysis |
| title_full_unstemmed | Determination of Saponin Content in Hang Maidong and Chuan Maidong via HPLC-ELSD Analysis |
| title_short | Determination of Saponin Content in Hang Maidong and Chuan Maidong via HPLC-ELSD Analysis |
| title_sort | determination of saponin content in hang maidong and chuan maidong via hplc elsd analysis |
| url | http://dx.doi.org/10.1155/2016/7214607 |
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