The mRNA Expression and Methylation Status in Imprinting Control Region of H19 Gene Between Cattle-Yak and Their Parents
The H19 gene, which is imprinted with preferential expression from the maternal allele, was one of the first identified imprinting genes in mammals. Recent studies revealed that correct imprinting of the H19 gene plays a vital role in human spermatogenesis. To investigate whether imprinting defects...
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KeAi Communications Co., Ltd.
2012-10-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311912601725 |
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| author | Ming-gui LI Zhen-shan LIU Zeng-xiang PAN Hua LUO Zhuang XIE Qi-fa LI |
| author_facet | Ming-gui LI Zhen-shan LIU Zeng-xiang PAN Hua LUO Zhuang XIE Qi-fa LI |
| author_sort | Ming-gui LI |
| collection | DOAJ |
| description | The H19 gene, which is imprinted with preferential expression from the maternal allele, was one of the first identified imprinting genes in mammals. Recent studies revealed that correct imprinting of the H19 gene plays a vital role in human spermatogenesis. To investigate whether imprinting defects were associated with the hybrid sterility of male cattle-yak, the methylation patterns of the H19 imprinting control region (ICR) and H19 mRNA expression in the testes of cattle-yak, yak, and cattle were examined. The results showed that the 3rd CCCTC-binding factor (CTCF) site of the H19 ICR was significantly hypomethylated in the testes of cattle-yak compared with yak or cattle. As expected, H19 was expressed at a significantly higher level in cattle-yak than in yak or cattle. These results suggest that imprinting defects of the CTCF-binding site in the H19 ICR were possibly associated with disturbed spermatogenesis of male cattle-yak. Thus, we propose that disorders in H19 imprinting, resulting in an increased H19 mRNA expression, might contribute to the sterility of F1 male hybrids between cattle and yak. |
| format | Article |
| id | doaj-art-efcd1809cdb247eba7dbf6fff8d36dce |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2012-10-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-efcd1809cdb247eba7dbf6fff8d36dce2025-08-20T03:57:55ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192012-10-0111101691169910.1016/S2095-3119(12)60172-5The mRNA Expression and Methylation Status in Imprinting Control Region of H19 Gene Between Cattle-Yak and Their ParentsMing-gui LI0Zhen-shan LIU1Zeng-xiang PAN2Hua LUO3Zhuang XIE4Qi-fa LI5College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaCorrespondence LI Qi-fa, Tel: +86-25-84395046, Fax: +86-25-84395314; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaThe H19 gene, which is imprinted with preferential expression from the maternal allele, was one of the first identified imprinting genes in mammals. Recent studies revealed that correct imprinting of the H19 gene plays a vital role in human spermatogenesis. To investigate whether imprinting defects were associated with the hybrid sterility of male cattle-yak, the methylation patterns of the H19 imprinting control region (ICR) and H19 mRNA expression in the testes of cattle-yak, yak, and cattle were examined. The results showed that the 3rd CCCTC-binding factor (CTCF) site of the H19 ICR was significantly hypomethylated in the testes of cattle-yak compared with yak or cattle. As expected, H19 was expressed at a significantly higher level in cattle-yak than in yak or cattle. These results suggest that imprinting defects of the CTCF-binding site in the H19 ICR were possibly associated with disturbed spermatogenesis of male cattle-yak. Thus, we propose that disorders in H19 imprinting, resulting in an increased H19 mRNA expression, might contribute to the sterility of F1 male hybrids between cattle and yak.http://www.sciencedirect.com/science/article/pii/S2095311912601725cattle-yakmale sterilityH19 ICRCTCF-binding sitemRNA expressionDNA methylation |
| spellingShingle | Ming-gui LI Zhen-shan LIU Zeng-xiang PAN Hua LUO Zhuang XIE Qi-fa LI The mRNA Expression and Methylation Status in Imprinting Control Region of H19 Gene Between Cattle-Yak and Their Parents Journal of Integrative Agriculture cattle-yak male sterility H19 ICR CTCF-binding site mRNA expression DNA methylation |
| title | The mRNA Expression and Methylation Status in Imprinting Control Region of H19 Gene Between Cattle-Yak and Their Parents |
| title_full | The mRNA Expression and Methylation Status in Imprinting Control Region of H19 Gene Between Cattle-Yak and Their Parents |
| title_fullStr | The mRNA Expression and Methylation Status in Imprinting Control Region of H19 Gene Between Cattle-Yak and Their Parents |
| title_full_unstemmed | The mRNA Expression and Methylation Status in Imprinting Control Region of H19 Gene Between Cattle-Yak and Their Parents |
| title_short | The mRNA Expression and Methylation Status in Imprinting Control Region of H19 Gene Between Cattle-Yak and Their Parents |
| title_sort | mrna expression and methylation status in imprinting control region of h19 gene between cattle yak and their parents |
| topic | cattle-yak male sterility H19 ICR CTCF-binding site mRNA expression DNA methylation |
| url | http://www.sciencedirect.com/science/article/pii/S2095311912601725 |
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