Magnetic control of membrane damage in early endosomes using internalized magnetic nanoparticles
Membrane stiffness is essential for cell migration, tumorigenesis, and development; however, the physical properties of intracellular membrane are poorly characterized. In this study, we internalized 20 nm magnetic nanoparticles (MNPs) into MCF7 human breast cancer cells and applied a magnetic field...
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| Format: | Article |
| Language: | English |
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Japan Society for Cell Biology
2024-12-01
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| Series: | Cell Structure and Function |
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| Online Access: | https://www.jstage.jst.go.jp/article/csf/50/1/50_24037/_html/-char/en |
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| author | Yuta Yonekawa Kazuki Oikawa Boldbaatar Bayarkhuu Kizuna Kobayashi Nana Saito Ibuki Oikawa Ryohei Yamada Yu-han Chen Koichi Oyanagi Yuji Shibasaki Satoru Kobayashi Yoko Shiba |
| author_facet | Yuta Yonekawa Kazuki Oikawa Boldbaatar Bayarkhuu Kizuna Kobayashi Nana Saito Ibuki Oikawa Ryohei Yamada Yu-han Chen Koichi Oyanagi Yuji Shibasaki Satoru Kobayashi Yoko Shiba |
| author_sort | Yuta Yonekawa |
| collection | DOAJ |
| description | Membrane stiffness is essential for cell migration, tumorigenesis, and development; however, the physical properties of intracellular membrane are poorly characterized. In this study, we internalized 20 nm magnetic nanoparticles (MNPs) into MCF7 human breast cancer cells and applied a magnetic field. We investigated whether magnetic field could induce membrane damage of the early endosomes by analyzing the colocalization of MNPs with galectin 3 (Gal3), a cytosolic protein recruited to the lumen of damaged organelles. We first tried to apply magnetic field by electromagnet, and found a direct-current (DC) magnetic field for five minutes increased the colocalization of the MNPs with Gal3, suggesting that the magnetic field damaged the endosomal membrane. We used a neodymium magnet to apply longer and stronger static magnetic fields. The static magnetic field more than 50 mT for five minutes started to damage endosomes, while 100 mT was the most effective. Longer exposure or higher magnetic field strengths did not induce further membrane damage. We confirmed that a Gal3 positive compartment was also positive for the early endosome marker, EEA1, suggesting that the external magnetic field induced membrane damage in the early endosomes. Our results indicate that a static magnetic field can control the membrane damage in early endosomes using internalized MNPs. Key words: magnetic nanoparticles, endosomes, membrane damage, organelle |
| format | Article |
| id | doaj-art-efa9bcfb63464fc4aece5d4e784cf584 |
| institution | DOAJ |
| issn | 0386-7196 1347-3700 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Japan Society for Cell Biology |
| record_format | Article |
| series | Cell Structure and Function |
| spelling | doaj-art-efa9bcfb63464fc4aece5d4e784cf5842025-08-20T03:12:36ZengJapan Society for Cell BiologyCell Structure and Function0386-71961347-37002024-12-01501253910.1247/csf.24037csfMagnetic control of membrane damage in early endosomes using internalized magnetic nanoparticlesYuta Yonekawa0Kazuki Oikawa1Boldbaatar Bayarkhuu2Kizuna Kobayashi3Nana Saito4Ibuki Oikawa5Ryohei Yamada6Yu-han Chen7Koichi Oyanagi8Yuji Shibasaki9Satoru Kobayashi10Yoko Shiba11Graduate Course in Biological Sciences, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate UniversityGraduate Course in Biological Sciences, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate UniversityGraduate Course in Biological Sciences, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate UniversityGraduate Course in Biological Sciences, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate UniversityGraduate Course in Biological Sciences, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate UniversityGraduate Course in Materials Science and Engineering, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate UniversityGraduate Course in Materials Science and Engineering, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate UniversityDepartment of Biochemical Science and Technology, National Chiayi UniversityGraduate Course in Materials Science and Engineering, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate UniversityGraduate Course in Chemistry, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate UniversityGraduate Course in Materials Science and Engineering, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate UniversityGraduate Course in Biological Sciences, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate UniversityMembrane stiffness is essential for cell migration, tumorigenesis, and development; however, the physical properties of intracellular membrane are poorly characterized. In this study, we internalized 20 nm magnetic nanoparticles (MNPs) into MCF7 human breast cancer cells and applied a magnetic field. We investigated whether magnetic field could induce membrane damage of the early endosomes by analyzing the colocalization of MNPs with galectin 3 (Gal3), a cytosolic protein recruited to the lumen of damaged organelles. We first tried to apply magnetic field by electromagnet, and found a direct-current (DC) magnetic field for five minutes increased the colocalization of the MNPs with Gal3, suggesting that the magnetic field damaged the endosomal membrane. We used a neodymium magnet to apply longer and stronger static magnetic fields. The static magnetic field more than 50 mT for five minutes started to damage endosomes, while 100 mT was the most effective. Longer exposure or higher magnetic field strengths did not induce further membrane damage. We confirmed that a Gal3 positive compartment was also positive for the early endosome marker, EEA1, suggesting that the external magnetic field induced membrane damage in the early endosomes. Our results indicate that a static magnetic field can control the membrane damage in early endosomes using internalized MNPs. Key words: magnetic nanoparticles, endosomes, membrane damage, organellehttps://www.jstage.jst.go.jp/article/csf/50/1/50_24037/_html/-char/enmagnetic nanoparticlesendosomesmembrane damageorganelle |
| spellingShingle | Yuta Yonekawa Kazuki Oikawa Boldbaatar Bayarkhuu Kizuna Kobayashi Nana Saito Ibuki Oikawa Ryohei Yamada Yu-han Chen Koichi Oyanagi Yuji Shibasaki Satoru Kobayashi Yoko Shiba Magnetic control of membrane damage in early endosomes using internalized magnetic nanoparticles Cell Structure and Function magnetic nanoparticles endosomes membrane damage organelle |
| title | Magnetic control of membrane damage in early endosomes using internalized magnetic nanoparticles |
| title_full | Magnetic control of membrane damage in early endosomes using internalized magnetic nanoparticles |
| title_fullStr | Magnetic control of membrane damage in early endosomes using internalized magnetic nanoparticles |
| title_full_unstemmed | Magnetic control of membrane damage in early endosomes using internalized magnetic nanoparticles |
| title_short | Magnetic control of membrane damage in early endosomes using internalized magnetic nanoparticles |
| title_sort | magnetic control of membrane damage in early endosomes using internalized magnetic nanoparticles |
| topic | magnetic nanoparticles endosomes membrane damage organelle |
| url | https://www.jstage.jst.go.jp/article/csf/50/1/50_24037/_html/-char/en |
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