Differential Immunogenicity and Protective Efficacy Elicited by MTO- and DMT-Adjuvanted CMFO Subunit Vaccines against Mycobacterium tuberculosis Infection
Tuberculosis (TB) remains a major and global problem of public health. An effective TB subunit vaccine is urgently needed. Proper selection of the delivery system for the vaccine is crucial for inducing an appropriate immune response tailored to control the target pathogen. In this study, we compare...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/2083793 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561466480263168 |
|---|---|
| author | Nadeem Ullah Ling Hao Yaqi Wu Yandi Zhang Qing Lei Jo-Lewis Banga Ndzouboukou Xiaosong Lin Xionglin Fan |
| author_facet | Nadeem Ullah Ling Hao Yaqi Wu Yandi Zhang Qing Lei Jo-Lewis Banga Ndzouboukou Xiaosong Lin Xionglin Fan |
| author_sort | Nadeem Ullah |
| collection | DOAJ |
| description | Tuberculosis (TB) remains a major and global problem of public health. An effective TB subunit vaccine is urgently needed. Proper selection of the delivery system for the vaccine is crucial for inducing an appropriate immune response tailored to control the target pathogen. In this study, we compared the immunogenicity and protective efficacy of CMFO subunit vaccines against primary progressive TB in two different adjuvant systems: the MTO oil-in-water (O/W) emulsion composed of monophosphoryl lipid A (MPL), trehalose-6,60-dibehenate (TDB), and oil in water emulsion MF59 and the DMT liposome containing dimethyldioctadecylammonium bromide (DDA), monophosphoryl lipid A (MPL), and trehalose-6,60-dibehenate (TDB). Our results demonstrated that the DMT-adjuvanted CMFO could confer more significant protection against M. tuberculosis infection than the CMFO/MTO did in mice. In particular, the adjuvant DMT showed a stronger ability than the O/W emulsion to adjuvant CMFO subunit vaccine and enhanced protection, attributed to elicit Th1-biased responses, strong Th1/Th17 cytokine responses, and IFN-γ+ or IL-2+ T cell responses. Therefore, our findings demonstrate that the liposome delivery system shows more effectiveness to adjuvant TB subunit vaccine than O/W emulsion and highlight the importance of adjuvant formulation for the better efficacy of a protein vaccine. |
| format | Article |
| id | doaj-art-ef9c98debc6f455aa092e21b03acd0e7 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-ef9c98debc6f455aa092e21b03acd0e72025-02-03T01:24:56ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/20837932083793Differential Immunogenicity and Protective Efficacy Elicited by MTO- and DMT-Adjuvanted CMFO Subunit Vaccines against Mycobacterium tuberculosis InfectionNadeem Ullah0Ling Hao1Yaqi Wu2Yandi Zhang3Qing Lei4Jo-Lewis Banga Ndzouboukou5Xiaosong Lin6Xionglin Fan7Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, ChinaDepartment of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, ChinaDepartment of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, ChinaDepartment of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, ChinaDepartment of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, ChinaDepartment of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, ChinaDepartment of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, ChinaDepartment of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, ChinaTuberculosis (TB) remains a major and global problem of public health. An effective TB subunit vaccine is urgently needed. Proper selection of the delivery system for the vaccine is crucial for inducing an appropriate immune response tailored to control the target pathogen. In this study, we compared the immunogenicity and protective efficacy of CMFO subunit vaccines against primary progressive TB in two different adjuvant systems: the MTO oil-in-water (O/W) emulsion composed of monophosphoryl lipid A (MPL), trehalose-6,60-dibehenate (TDB), and oil in water emulsion MF59 and the DMT liposome containing dimethyldioctadecylammonium bromide (DDA), monophosphoryl lipid A (MPL), and trehalose-6,60-dibehenate (TDB). Our results demonstrated that the DMT-adjuvanted CMFO could confer more significant protection against M. tuberculosis infection than the CMFO/MTO did in mice. In particular, the adjuvant DMT showed a stronger ability than the O/W emulsion to adjuvant CMFO subunit vaccine and enhanced protection, attributed to elicit Th1-biased responses, strong Th1/Th17 cytokine responses, and IFN-γ+ or IL-2+ T cell responses. Therefore, our findings demonstrate that the liposome delivery system shows more effectiveness to adjuvant TB subunit vaccine than O/W emulsion and highlight the importance of adjuvant formulation for the better efficacy of a protein vaccine.http://dx.doi.org/10.1155/2020/2083793 |
| spellingShingle | Nadeem Ullah Ling Hao Yaqi Wu Yandi Zhang Qing Lei Jo-Lewis Banga Ndzouboukou Xiaosong Lin Xionglin Fan Differential Immunogenicity and Protective Efficacy Elicited by MTO- and DMT-Adjuvanted CMFO Subunit Vaccines against Mycobacterium tuberculosis Infection Journal of Immunology Research |
| title | Differential Immunogenicity and Protective Efficacy Elicited by MTO- and DMT-Adjuvanted CMFO Subunit Vaccines against Mycobacterium tuberculosis Infection |
| title_full | Differential Immunogenicity and Protective Efficacy Elicited by MTO- and DMT-Adjuvanted CMFO Subunit Vaccines against Mycobacterium tuberculosis Infection |
| title_fullStr | Differential Immunogenicity and Protective Efficacy Elicited by MTO- and DMT-Adjuvanted CMFO Subunit Vaccines against Mycobacterium tuberculosis Infection |
| title_full_unstemmed | Differential Immunogenicity and Protective Efficacy Elicited by MTO- and DMT-Adjuvanted CMFO Subunit Vaccines against Mycobacterium tuberculosis Infection |
| title_short | Differential Immunogenicity and Protective Efficacy Elicited by MTO- and DMT-Adjuvanted CMFO Subunit Vaccines against Mycobacterium tuberculosis Infection |
| title_sort | differential immunogenicity and protective efficacy elicited by mto and dmt adjuvanted cmfo subunit vaccines against mycobacterium tuberculosis infection |
| url | http://dx.doi.org/10.1155/2020/2083793 |
| work_keys_str_mv | AT nadeemullah differentialimmunogenicityandprotectiveefficacyelicitedbymtoanddmtadjuvantedcmfosubunitvaccinesagainstmycobacteriumtuberculosisinfection AT linghao differentialimmunogenicityandprotectiveefficacyelicitedbymtoanddmtadjuvantedcmfosubunitvaccinesagainstmycobacteriumtuberculosisinfection AT yaqiwu differentialimmunogenicityandprotectiveefficacyelicitedbymtoanddmtadjuvantedcmfosubunitvaccinesagainstmycobacteriumtuberculosisinfection AT yandizhang differentialimmunogenicityandprotectiveefficacyelicitedbymtoanddmtadjuvantedcmfosubunitvaccinesagainstmycobacteriumtuberculosisinfection AT qinglei differentialimmunogenicityandprotectiveefficacyelicitedbymtoanddmtadjuvantedcmfosubunitvaccinesagainstmycobacteriumtuberculosisinfection AT jolewisbangandzouboukou differentialimmunogenicityandprotectiveefficacyelicitedbymtoanddmtadjuvantedcmfosubunitvaccinesagainstmycobacteriumtuberculosisinfection AT xiaosonglin differentialimmunogenicityandprotectiveefficacyelicitedbymtoanddmtadjuvantedcmfosubunitvaccinesagainstmycobacteriumtuberculosisinfection AT xionglinfan differentialimmunogenicityandprotectiveefficacyelicitedbymtoanddmtadjuvantedcmfosubunitvaccinesagainstmycobacteriumtuberculosisinfection |