Comprehensive Chloroplast Genome Analysis of Four <i>Callitriche</i> (Plantaginaceae) Species for Phylogenetic and Conservation Insights
<i>Callitriche</i> species are capable of purifying water, promoting wetland restoration, and providing natural shelters. Moreover, they can be utilized as horticultural plants for landscape greening. However, due to the threats of climate change and environmental degradation, some speci...
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2025-01-01
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| Series: | Horticulturae |
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| author | Zirui Zhang Wenbo Shi Siqi Hu Lanruo Mou Chao Shi Bingyue Zhu Jing Yang |
| author_facet | Zirui Zhang Wenbo Shi Siqi Hu Lanruo Mou Chao Shi Bingyue Zhu Jing Yang |
| author_sort | Zirui Zhang |
| collection | DOAJ |
| description | <i>Callitriche</i> species are capable of purifying water, promoting wetland restoration, and providing natural shelters. Moreover, they can be utilized as horticultural plants for landscape greening. However, due to the threats of climate change and environmental degradation, some species within this genus have been listed as endangered. This study utilizes chloroplast genome analysis to provide molecular evidence for the classification and conservation of these species. We conducted a comprehensive sequencing and characterization of the complete chloroplast genomes of four species within the genus <i>Callitriche</i>: <i>C. cophocarpa</i>, <i>C. hermaphroditica</i>, <i>C. palustris</i>, and <i>C. stagnalis</i>. The genome sizes ranged from 150,042 to 150,879 bp, with a GC content of 37.5–37.8% and between 131 and 132 genes. Comparative genomic analysis revealed several highly variable intergenic regions (e.g., <i>rps</i>16–<i>psb</i>K, <i>trn</i>S-GCU–<i>trn</i>G-UCC, <i>ccs</i>A–<i>ndh</i>D, <i>ndh</i>F–<i>rpl</i>32, and <i>trn</i>N-UGG) and the <i>ycf</i>1 gene, highlighting their potential as phylogenetic markers. Phylogenetic analyses confirmed the monophyly of <i>Callitriche</i> and supported <i>C. hermaphroditica</i> as an early-diverging lineage within the genus. Notably, the phylogeny also resolved <i>Hemiphragma</i> and <i>Veronicastrum</i> as sister taxa, contributing insights into evolutionary relationships within Plantaginaceae. This study provides comprehensive chloroplast genomic data for <i>Callitriche</i>, offering valuable molecular markers for phylogenetic research, taxonomic clarification, and conservation of this ecologically significant genus. |
| format | Article |
| id | doaj-art-ef63200fff1246088c7e8107d44bcda3 |
| institution | Kabale University |
| issn | 2311-7524 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj-art-ef63200fff1246088c7e8107d44bcda32025-01-24T13:34:39ZengMDPI AGHorticulturae2311-75242025-01-011116610.3390/horticulturae11010066Comprehensive Chloroplast Genome Analysis of Four <i>Callitriche</i> (Plantaginaceae) Species for Phylogenetic and Conservation InsightsZirui Zhang0Wenbo Shi1Siqi Hu2Lanruo Mou3Chao Shi4Bingyue Zhu5Jing Yang6College of Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, ChinaCollege of Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, ChinaCollege of Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, ChinaCollege of Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, ChinaCollege of Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, ChinaCollege of Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, ChinaCollege of Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China<i>Callitriche</i> species are capable of purifying water, promoting wetland restoration, and providing natural shelters. Moreover, they can be utilized as horticultural plants for landscape greening. However, due to the threats of climate change and environmental degradation, some species within this genus have been listed as endangered. This study utilizes chloroplast genome analysis to provide molecular evidence for the classification and conservation of these species. We conducted a comprehensive sequencing and characterization of the complete chloroplast genomes of four species within the genus <i>Callitriche</i>: <i>C. cophocarpa</i>, <i>C. hermaphroditica</i>, <i>C. palustris</i>, and <i>C. stagnalis</i>. The genome sizes ranged from 150,042 to 150,879 bp, with a GC content of 37.5–37.8% and between 131 and 132 genes. Comparative genomic analysis revealed several highly variable intergenic regions (e.g., <i>rps</i>16–<i>psb</i>K, <i>trn</i>S-GCU–<i>trn</i>G-UCC, <i>ccs</i>A–<i>ndh</i>D, <i>ndh</i>F–<i>rpl</i>32, and <i>trn</i>N-UGG) and the <i>ycf</i>1 gene, highlighting their potential as phylogenetic markers. Phylogenetic analyses confirmed the monophyly of <i>Callitriche</i> and supported <i>C. hermaphroditica</i> as an early-diverging lineage within the genus. Notably, the phylogeny also resolved <i>Hemiphragma</i> and <i>Veronicastrum</i> as sister taxa, contributing insights into evolutionary relationships within Plantaginaceae. This study provides comprehensive chloroplast genomic data for <i>Callitriche</i>, offering valuable molecular markers for phylogenetic research, taxonomic clarification, and conservation of this ecologically significant genus.https://www.mdpi.com/2311-7524/11/1/66<i>Callitriche</i>chloroplast genomecomparative analysisphylogeny |
| spellingShingle | Zirui Zhang Wenbo Shi Siqi Hu Lanruo Mou Chao Shi Bingyue Zhu Jing Yang Comprehensive Chloroplast Genome Analysis of Four <i>Callitriche</i> (Plantaginaceae) Species for Phylogenetic and Conservation Insights Horticulturae <i>Callitriche</i> chloroplast genome comparative analysis phylogeny |
| title | Comprehensive Chloroplast Genome Analysis of Four <i>Callitriche</i> (Plantaginaceae) Species for Phylogenetic and Conservation Insights |
| title_full | Comprehensive Chloroplast Genome Analysis of Four <i>Callitriche</i> (Plantaginaceae) Species for Phylogenetic and Conservation Insights |
| title_fullStr | Comprehensive Chloroplast Genome Analysis of Four <i>Callitriche</i> (Plantaginaceae) Species for Phylogenetic and Conservation Insights |
| title_full_unstemmed | Comprehensive Chloroplast Genome Analysis of Four <i>Callitriche</i> (Plantaginaceae) Species for Phylogenetic and Conservation Insights |
| title_short | Comprehensive Chloroplast Genome Analysis of Four <i>Callitriche</i> (Plantaginaceae) Species for Phylogenetic and Conservation Insights |
| title_sort | comprehensive chloroplast genome analysis of four i callitriche i plantaginaceae species for phylogenetic and conservation insights |
| topic | <i>Callitriche</i> chloroplast genome comparative analysis phylogeny |
| url | https://www.mdpi.com/2311-7524/11/1/66 |
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