Genome-wide identification of the nuclear redox protein gene family revealed its potential role in drought stress tolerance in rice

Genome-wide identification of the nuclear redox protein gene family revealed its potential role in drought stress tolerance in rice

IntroductionThioredoxins (TRX) are redox-active proteins critical for plant stress adaptation. As a TRX family member, nucleoredoxin (NRX) maintains drought-induced redox homeostasis, yet its genome-wide characterization in rice remains uninvestigated.MethodsUsing HMMER3.0 (E-value <1e-5) and...

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Bibliographic Details
Main Authors: Zijie Liu, Xingfei Zheng, Dabing Yang, Lanzhi Li, Hexing Yin
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Plant Science
Subjects:
rice
nucleoredoxin gene family
drought stress
genome-wide identification
expression pattern
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2025.1562718/full
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Summary:IntroductionThioredoxins (TRX) are redox-active proteins critical for plant stress adaptation. As a TRX family member, nucleoredoxin (NRX) maintains drought-induced redox homeostasis, yet its genome-wide characterization in rice remains uninvestigated.MethodsUsing HMMER3.0 (E-value <1e-5) and TBtools, we identified OsNRX genes across three rice varieties (Minghui63, Nipponbare, 9311). Conserved domains were verified by SMART/CDD, while promoter cis-elements were systematically predicted with PlantCARE. Tissue-specific expression patterns were analyzed using RiceXPro data, and drought responses were quantified via qRT-PCR in drought-tolerant (Jiangnong Zao 1B) versus sensitive (TAISEN GLUTINOUS YU 1157) varieties under PEG6000 stress.ResultsTen OsNRX genes were classified into three subfamilies (NRX1/NRX2/NRX3) exhibiting conserved domain architectures. Promoter analysis identified abundant stress-responsive elements (ABRE, MBS) and phytohormone signals (ABA/JA/SA). Tissue-specific expression profiles revealed NRX1a dominance in roots/hulls, versus NRX1b/NRX2 enrichment in endosperm. Drought stress triggered rapid OsNRX upregulation (20-53-fold within 3-6h), with tolerant varieties showing earlier NRX2 activation than sensitive counterparts.DiscussionOsNRX genes exhibit dynamic drought-responsive regulation, while their spatiotemporal expression in glumes, embryos, and endosperm suggests potential dual roles in stress adaptation and grain development. These results provide molecular targets for improving drought resilience in rice breeding.
ISSN:1664-462X

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