Identification, Pathogenicity, and Antimicrobial Resistance Analysis of Bacterial Pathogenesis <i>Aeromonas hydrophila</i> from Hybrid Sturgeon (<i>Huso dauricus</i> ♀ × <i>A. schrenckii</i> ♂) in Zhejiang, China

Identification, Pathogenicity, and Antimicrobial Resistance Analysis of Bacterial Pathogenesis <i>Aeromonas hydrophila</i> from Hybrid Sturgeon (<i>Huso dauricus</i> ♀ × <i>A. schrenckii</i> ♂) in Zhejiang, China

In 2019, a disease outbreak struck a hybrid sturgeon farm (<i>Huso dauricus</i> ♀ × <i>A. schrenckii</i> ♂) in Tiantai, Zhejiang province, leading to the deaths of 8000 sturgeons. The sturgeons exhibited reduced appetite, lethargic and uncoordinated swimming, and physical sig...

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Main Authors: Haojie Hu, Xinzhi Weng, Gang Pang, Xiaobing Li, Jing Xia, Xiu Gao, Jie He, Ji Li, Dong Qian
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Microorganisms
Subjects:
hybrid sturgeon
<i>Aeromonas hydrophila</i>
identification
pathogenicity
antimicrobial resistance
Online Access:https://www.mdpi.com/2076-2607/13/2/278
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Summary:In 2019, a disease outbreak struck a hybrid sturgeon farm (<i>Huso dauricus</i> ♀ × <i>A. schrenckii</i> ♂) in Tiantai, Zhejiang province, leading to the deaths of 8000 sturgeons. The sturgeons exhibited reduced appetite, lethargic and uncoordinated swimming, and physical signs such as reddish petechiae and ulcers on the body and fins. Hemorrhagic spots were observed on the kidneys, spleen, and gonads, alongside reddish intestines with hemorrhagic ascites in the abdominal cavity. ST-1902 was isolated and identified as <i>Aeromonas hydrophila</i> through physiological and biochemical characterization and 16S rDNA sequence analysis. The pathogenicity of ST-1902 was confirmed through a challenge test, with a median lethal dosage (LD50) of 7.9 × 10<sup>6</sup> CFU/IND. Histopathological examination showed hyperplasia and neoplasm-like changes in the epicedial mesothelial tissues, enlarged and necrosis renal tissue, and serious hemosiderosis in spleen and gills. Virulent genes (<i>Aer</i>, <i>Epa</i>, <i>Alt</i>, <i>Hly</i>, and <i>Act</i>) were detected in ST-1902, corresponding to typical β-hemolysis, extracellular protease, and enterotoxin. Moreover, antimicrobial experiment detection indicated ST-1902 is sensitive to quinolones and phenicols but resistant to sulfamethoxazole, aminoglycoside antibiotics with <i>Sul1</i>, and <i>Intl</i> and <i>Ant</i> (<i>3”</i>)-I. These results suggest that <i>A. hydrophila</i> was the causative agent of the sturgeon disease and highlight the emerging threat it poses to the sturgeon industry.
ISSN:2076-2607

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