Enhancing Bioactive Cordycepin Production via Precision Fermentation with an Engineered <i>Aspergillus oryzae</i>
An optimal culture medium and highly stable biometabolites are important in industrial production processes. The response surface methodology with a Box–Behnken design was performed to determine the optimal culture medium of an engineered <i>Aspergillus oryzae</i> strain for cordycepin p...
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MDPI AG
2025-01-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/11/1/32 |
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| author | Jutamas Anantayanon Warinthon Chamkhuy Nakul Rattanaphan Sarocha Panchanawaporn Kobkul Laoteng Sukanya Jeennor |
| author_facet | Jutamas Anantayanon Warinthon Chamkhuy Nakul Rattanaphan Sarocha Panchanawaporn Kobkul Laoteng Sukanya Jeennor |
| author_sort | Jutamas Anantayanon |
| collection | DOAJ |
| description | An optimal culture medium and highly stable biometabolites are important in industrial production processes. The response surface methodology with a Box–Behnken design was performed to determine the optimal culture medium of an engineered <i>Aspergillus oryzae</i> strain for cordycepin production by submerged fermentation. The influences of glucose, yeast extract, and adenine concentrations on cordycepin production were explored, and their concentrations were used for experimental design. The results reveal that the optimal culture components involved 30.0 g/L of glucose, 9.8 g/L of yeast extract, and 1.5 g/L of adenine. As predicted, the maximum cordycepin concentration (1724.53 ± 18.30 mg/L) was obtained with a short fermentation time (2 days). A significant increase in cordycepin yield (>50% increase) was observed in the culture grown in the optimized culture medium compared to that grown in the basal medium. A xanthine oxidase inhibitory activity assay demonstrated that the cordycepin product had a pharmacological function. It exhibited strong stability under high thermal and acidic conditions, with over 95% product recovery. The findings of this study are valuable for developing cost-effective processes for producing health-benefiting products. |
| format | Article |
| id | doaj-art-ef28e1c49f494e6893f415731325c284 |
| institution | Kabale University |
| issn | 2311-5637 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj-art-ef28e1c49f494e6893f415731325c2842025-01-24T13:32:07ZengMDPI AGFermentation2311-56372025-01-011113210.3390/fermentation11010032Enhancing Bioactive Cordycepin Production via Precision Fermentation with an Engineered <i>Aspergillus oryzae</i>Jutamas Anantayanon0Warinthon Chamkhuy1Nakul Rattanaphan2Sarocha Panchanawaporn3Kobkul Laoteng4Sukanya Jeennor5Industrial Bioprocess Technology Research Team, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang 12120, Pathum Thani, ThailandIndustrial Bioprocess Technology Research Team, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang 12120, Pathum Thani, ThailandIndustrial Bioprocess Technology Research Team, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang 12120, Pathum Thani, ThailandIndustrial Bioprocess Technology Research Team, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang 12120, Pathum Thani, ThailandIndustrial Bioprocess Technology Research Team, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang 12120, Pathum Thani, ThailandIndustrial Bioprocess Technology Research Team, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang 12120, Pathum Thani, ThailandAn optimal culture medium and highly stable biometabolites are important in industrial production processes. The response surface methodology with a Box–Behnken design was performed to determine the optimal culture medium of an engineered <i>Aspergillus oryzae</i> strain for cordycepin production by submerged fermentation. The influences of glucose, yeast extract, and adenine concentrations on cordycepin production were explored, and their concentrations were used for experimental design. The results reveal that the optimal culture components involved 30.0 g/L of glucose, 9.8 g/L of yeast extract, and 1.5 g/L of adenine. As predicted, the maximum cordycepin concentration (1724.53 ± 18.30 mg/L) was obtained with a short fermentation time (2 days). A significant increase in cordycepin yield (>50% increase) was observed in the culture grown in the optimized culture medium compared to that grown in the basal medium. A xanthine oxidase inhibitory activity assay demonstrated that the cordycepin product had a pharmacological function. It exhibited strong stability under high thermal and acidic conditions, with over 95% product recovery. The findings of this study are valuable for developing cost-effective processes for producing health-benefiting products.https://www.mdpi.com/2311-5637/11/1/32<i>Aspergillus oryzae</i>cordycepinsynthetic biologysubmerged fermentationBox–Behnken designresponse surface methodology |
| spellingShingle | Jutamas Anantayanon Warinthon Chamkhuy Nakul Rattanaphan Sarocha Panchanawaporn Kobkul Laoteng Sukanya Jeennor Enhancing Bioactive Cordycepin Production via Precision Fermentation with an Engineered <i>Aspergillus oryzae</i> Fermentation <i>Aspergillus oryzae</i> cordycepin synthetic biology submerged fermentation Box–Behnken design response surface methodology |
| title | Enhancing Bioactive Cordycepin Production via Precision Fermentation with an Engineered <i>Aspergillus oryzae</i> |
| title_full | Enhancing Bioactive Cordycepin Production via Precision Fermentation with an Engineered <i>Aspergillus oryzae</i> |
| title_fullStr | Enhancing Bioactive Cordycepin Production via Precision Fermentation with an Engineered <i>Aspergillus oryzae</i> |
| title_full_unstemmed | Enhancing Bioactive Cordycepin Production via Precision Fermentation with an Engineered <i>Aspergillus oryzae</i> |
| title_short | Enhancing Bioactive Cordycepin Production via Precision Fermentation with an Engineered <i>Aspergillus oryzae</i> |
| title_sort | enhancing bioactive cordycepin production via precision fermentation with an engineered i aspergillus oryzae i |
| topic | <i>Aspergillus oryzae</i> cordycepin synthetic biology submerged fermentation Box–Behnken design response surface methodology |
| url | https://www.mdpi.com/2311-5637/11/1/32 |
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