Effect of attenueted parapoxvirus ovis on levels of some serum cytokines and antibody response in sheep
<b>Aim:</b> Parapoxvirus ovis (ORFV) infection is one of important contagious viral diseases of small ruminants. The aim of this study was to determine the concentration of cytokine and neutralizing antibody in blood serum of sheep that were carried out attenuated orf virus vaccine strai...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
2022-06-01
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1394 |
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| author | Kamil Atlı Atilla Şimşek |
| author_facet | Kamil Atlı Atilla Şimşek |
| author_sort | Kamil Atlı |
| collection | DOAJ |
| description | <b>Aim:</b> Parapoxvirus ovis (ORFV) infection is one of important contagious
viral diseases of small ruminants. The aim of this study was to determine the
concentration of cytokine and neutralizing antibody in blood serum of sheep
that were carried out attenuated orf virus vaccine strain.<p>
<b>Materials and Methods:</b> In this study, 10 male Akkaraman sheep which are
6-8 months of age and tested negative for antibodies against ORFV were used.
Scarification was applied with 0.5 ml of attenuated ORFV to each animal.
Serum samples were obtained from vena jugularis at 0. hour (control) before
the inoculation and at the following 6., 12., 24., 48., 72., 96., 120. hours, 7.,
14., 21., 30., 60., 90., 120., 150. and 180. days. Neutralizing antibody presence
was investigated in obtained serums. In addition, levels of IL-1?, IL-12, IFN-γ,
TNF-α from proinflammatory cytokines (Th1) and IL-4 and IL-10 from antiinflammatory
cytokines (Th2) were determined by ELISA.<p>
<b>Results:</b> ORFV caused fluctiations but no statistically significant change
in levels of IL-1β, IL-12, IFNγ, TNF-α and IL-10 were detected compared to
control sera (p>0,05), and significant increase in IL-4 level was detected
in the 30<sup>th</sup> day (p<0,05). As a result of the study, attenuated live ORFV
administration and have no effect on levels of some serum cytokine except
IL-4 and neutralizing antibodies.<p>
<b>Conclusion:</b> It was concluded that the vaccines against ORFV infection
considered to be used in the future should be applied after the effects on the
immune system have been clearly demonstrated. |
| format | Article |
| id | doaj-art-ef22a6738fc4416ba0c6b525c346891e |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publishDate | 2022-06-01 |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-ef22a6738fc4416ba0c6b525c346891e2025-02-03T11:25:37ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532022-06-013821221301394Effect of attenueted parapoxvirus ovis on levels of some serum cytokines and antibody response in sheepKamil Atlı0Atilla Şimşek1Burdur Mehmet Akif Ersoy University, Veterinary Faculty, Department of Virology, Burdur, TurkeySelcuk University, Veterinary Faculty, Department of Virology, Konya, Turkey<b>Aim:</b> Parapoxvirus ovis (ORFV) infection is one of important contagious viral diseases of small ruminants. The aim of this study was to determine the concentration of cytokine and neutralizing antibody in blood serum of sheep that were carried out attenuated orf virus vaccine strain.<p> <b>Materials and Methods:</b> In this study, 10 male Akkaraman sheep which are 6-8 months of age and tested negative for antibodies against ORFV were used. Scarification was applied with 0.5 ml of attenuated ORFV to each animal. Serum samples were obtained from vena jugularis at 0. hour (control) before the inoculation and at the following 6., 12., 24., 48., 72., 96., 120. hours, 7., 14., 21., 30., 60., 90., 120., 150. and 180. days. Neutralizing antibody presence was investigated in obtained serums. In addition, levels of IL-1?, IL-12, IFN-γ, TNF-α from proinflammatory cytokines (Th1) and IL-4 and IL-10 from antiinflammatory cytokines (Th2) were determined by ELISA.<p> <b>Results:</b> ORFV caused fluctiations but no statistically significant change in levels of IL-1β, IL-12, IFNγ, TNF-α and IL-10 were detected compared to control sera (p>0,05), and significant increase in IL-4 level was detected in the 30<sup>th</sup> day (p<0,05). As a result of the study, attenuated live ORFV administration and have no effect on levels of some serum cytokine except IL-4 and neutralizing antibodies.<p> <b>Conclusion:</b> It was concluded that the vaccines against ORFV infection considered to be used in the future should be applied after the effects on the immune system have been clearly demonstrated.http://eurasianjvetsci.org/pdf.php3?id=1394orfvattenuated straincell culturecytokinesheep |
| spellingShingle | Kamil Atlı Atilla Şimşek Effect of attenueted parapoxvirus ovis on levels of some serum cytokines and antibody response in sheep Eurasian Journal of Veterinary Sciences orfv attenuated strain cell culture cytokine sheep |
| title | Effect of attenueted parapoxvirus ovis on levels of some serum cytokines and antibody response in sheep |
| title_full | Effect of attenueted parapoxvirus ovis on levels of some serum cytokines and antibody response in sheep |
| title_fullStr | Effect of attenueted parapoxvirus ovis on levels of some serum cytokines and antibody response in sheep |
| title_full_unstemmed | Effect of attenueted parapoxvirus ovis on levels of some serum cytokines and antibody response in sheep |
| title_short | Effect of attenueted parapoxvirus ovis on levels of some serum cytokines and antibody response in sheep |
| title_sort | effect of attenueted parapoxvirus ovis on levels of some serum cytokines and antibody response in sheep |
| topic | orfv attenuated strain cell culture cytokine sheep |
| url | http://eurasianjvetsci.org/pdf.php3?id=1394 |
| work_keys_str_mv | AT kamilatlı effectofattenuetedparapoxvirusovisonlevelsofsomeserumcytokinesandantibodyresponseinsheep AT atillasimsek effectofattenuetedparapoxvirusovisonlevelsofsomeserumcytokinesandantibodyresponseinsheep |