A Reusable Capillary Flow-Driven Microfluidic System for Abscisic Acid Detection Using a Competitive Immunoassay
Point-of-care (PoC) devices offer a promising solution for fast, portable, and easy-to-use diagnostics. These characteristics are particularly relevant in agrifood fields like viticulture where the early detection of plant stresses is crucial to crop yield. Microfluidics, with its low reagent volume...
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MDPI AG
2025-01-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/25/2/411 |
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| author | Cristiana Domingues Marta S. C. Rodrigues Pedro G. M. Condelipes Ana Margarida Fortes Virginia Chu João Pedro Conde |
| author_facet | Cristiana Domingues Marta S. C. Rodrigues Pedro G. M. Condelipes Ana Margarida Fortes Virginia Chu João Pedro Conde |
| author_sort | Cristiana Domingues |
| collection | DOAJ |
| description | Point-of-care (PoC) devices offer a promising solution for fast, portable, and easy-to-use diagnostics. These characteristics are particularly relevant in agrifood fields like viticulture where the early detection of plant stresses is crucial to crop yield. Microfluidics, with its low reagent volume requirements, is well-suited for such applications. Self-driven microfluidic devices, which rely on capillary forces for fluid motion, offer an attractive alternative by eliminating the need for external pumps and complex fluid control systems. However, traditional microfluidic prototyping materials like polydimethylsiloxane (PDMS) present challenges due to their hydrophobic nature. This paper presents the development of a reusable, portable, capillary-driven microfluidic platform based on a PDMS-PEG (polyethylene glycol) copolymer designed for the rapid low-cost detection of abscisic acid (ABA), a key biomarker for the onset of ripening of non-climacteric fruits and drought stress in vines. By employing passive fluid transport mechanisms, such as capillary-driven sequential flow, this platform enables precise biological and chemical screenings while maintaining portability and ease of use. A simplified field-ready sample processing method is used to prepare the grapes for analysis. |
| format | Article |
| id | doaj-art-ef1b5f6dafdc4bfb8288168fa8fd8bbe |
| institution | Kabale University |
| issn | 1424-8220 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj-art-ef1b5f6dafdc4bfb8288168fa8fd8bbe2025-01-24T13:48:49ZengMDPI AGSensors1424-82202025-01-0125241110.3390/s25020411A Reusable Capillary Flow-Driven Microfluidic System for Abscisic Acid Detection Using a Competitive ImmunoassayCristiana Domingues0Marta S. C. Rodrigues1Pedro G. M. Condelipes2Ana Margarida Fortes3Virginia Chu4João Pedro Conde5Instituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias (INESC-MN), Rua Alves Redol, 1000-029 Lisbon, PortugalInstituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias (INESC-MN), Rua Alves Redol, 1000-029 Lisbon, PortugalInstituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias (INESC-MN), Rua Alves Redol, 1000-029 Lisbon, PortugalInstituto de Biossistemas e Ciências Integrativas (BioISI), Faculdade de Ciências de Lisboa, Universidade de Lisboa, 1749-016 Lisbon, PortugalInstituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias (INESC-MN), Rua Alves Redol, 1000-029 Lisbon, PortugalInstituto de Engenharia de Sistemas e Computadores–Microsistemas e Nanotecnologias (INESC-MN), Rua Alves Redol, 1000-029 Lisbon, PortugalPoint-of-care (PoC) devices offer a promising solution for fast, portable, and easy-to-use diagnostics. These characteristics are particularly relevant in agrifood fields like viticulture where the early detection of plant stresses is crucial to crop yield. Microfluidics, with its low reagent volume requirements, is well-suited for such applications. Self-driven microfluidic devices, which rely on capillary forces for fluid motion, offer an attractive alternative by eliminating the need for external pumps and complex fluid control systems. However, traditional microfluidic prototyping materials like polydimethylsiloxane (PDMS) present challenges due to their hydrophobic nature. This paper presents the development of a reusable, portable, capillary-driven microfluidic platform based on a PDMS-PEG (polyethylene glycol) copolymer designed for the rapid low-cost detection of abscisic acid (ABA), a key biomarker for the onset of ripening of non-climacteric fruits and drought stress in vines. By employing passive fluid transport mechanisms, such as capillary-driven sequential flow, this platform enables precise biological and chemical screenings while maintaining portability and ease of use. A simplified field-ready sample processing method is used to prepare the grapes for analysis.https://www.mdpi.com/1424-8220/25/2/411microfluidicsabscisic acidcapillarityhydrophilic polydimethylsiloxane copolymer (PDMS-PEG)portability |
| spellingShingle | Cristiana Domingues Marta S. C. Rodrigues Pedro G. M. Condelipes Ana Margarida Fortes Virginia Chu João Pedro Conde A Reusable Capillary Flow-Driven Microfluidic System for Abscisic Acid Detection Using a Competitive Immunoassay Sensors microfluidics abscisic acid capillarity hydrophilic polydimethylsiloxane copolymer (PDMS-PEG) portability |
| title | A Reusable Capillary Flow-Driven Microfluidic System for Abscisic Acid Detection Using a Competitive Immunoassay |
| title_full | A Reusable Capillary Flow-Driven Microfluidic System for Abscisic Acid Detection Using a Competitive Immunoassay |
| title_fullStr | A Reusable Capillary Flow-Driven Microfluidic System for Abscisic Acid Detection Using a Competitive Immunoassay |
| title_full_unstemmed | A Reusable Capillary Flow-Driven Microfluidic System for Abscisic Acid Detection Using a Competitive Immunoassay |
| title_short | A Reusable Capillary Flow-Driven Microfluidic System for Abscisic Acid Detection Using a Competitive Immunoassay |
| title_sort | reusable capillary flow driven microfluidic system for abscisic acid detection using a competitive immunoassay |
| topic | microfluidics abscisic acid capillarity hydrophilic polydimethylsiloxane copolymer (PDMS-PEG) portability |
| url | https://www.mdpi.com/1424-8220/25/2/411 |
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