Immune responses and resistance of white shrimp (Litopenaeus vannamei) fed Probiotic Bacillus sp NP5 and prebiotic honey against White Spot Syndrome Virus infection
ABSTRACT White spot disease caused by White Spot Syndrome Virus (WSSV) is the most serious viral disease and has a major impact on the decline in production of white shrimp farm. Improving the immune response through the application of prebiotic, probiotic and synbiotic is expected to be on...
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Asosiasi Sains Akuakultur Indonesia
2020-09-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/28498 |
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| author | Widanarni Widanarni Dewi Rahmi Muhamad Gustilatov Sukenda Sukenda Diah Ayu Satyari Utami |
| author_facet | Widanarni Widanarni Dewi Rahmi Muhamad Gustilatov Sukenda Sukenda Diah Ayu Satyari Utami |
| author_sort | Widanarni Widanarni |
| collection | DOAJ |
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ABSTRACT
White spot disease caused by White Spot Syndrome Virus (WSSV) is the most serious viral disease and has a major impact on the decline in production of white shrimp farm. Improving the immune response through the application of prebiotic, probiotic and synbiotic is expected to be one of the environmentally friendly alternatives to prevent the disease. This study aimed to evaluate the effect of administrating Baccillus sp. NP5 probiotic, honey prebiotic and a combination both (synbiotic) in enhancing immune response and resistence of white shrimp to WSSV infection. This study consisted of five treatments and three replications, namely positive control (feeding without probiotics, prebiotics, and synbiotics then challenged with WSSV), negative control (feeding without probiotics, prebiotics, synbiotics, then injected with PBS), pro (feeding with the addition of Bacillus sp. NP5 probiotics then challenged against WSSV), pre (feeding with the addition of honey prebiotics then challenged against WSSV), and sin (feeding with the addition of synbiotics then challenged against WSSV). White shrimp with an average weight of 1.8±0.06 gram/shrimp were reared at a density of 15 shrimps per aquarium (60 cm x 30 cm x 30 cm and water height of 20 cm) for eight weeks, then challenge against WSSV at lethal dose (LD50) dose as much as 0.1 ml per shrimp. The results showed that the immune response and resistence of white shrimp after treatments of probiotics, prebiotics, and synbiotics were better than those in controls with the optimal found in prebiotics treatment.
Keywords: Bacillus sp. NP5, honey, immune response, white shrimp, WSSV
ABSTRAK
White spot disease yang disebabkan oleh infeksi white spot syndrome virus (WSSV) merupakan penyakit viral paling serius dan berdampak besar terhadap penurunan produksi budidaya udang vaname. Perbaikan respons imun melalui aplikasi probiotik, prebiotik dan sinbiotik dapat menjadi salah satu alternatif ramah lingkungan untuk pencegahan serangan penyakit tersebut. Penelitian ini bertujuan untuk mengevaluasi pengaruh pemberian probiotik Bacillus sp. NP5, prebiotik madu dan gabungan keduanya (sinbiotik) dalam meningkatkan respons imun dan resistansi udang vaname terhadap infeksi WSSV. Penelitian ini terdiri dari lima perlakuan dan tiga ulangan yaitu kontrol positif (pemberian pakan tanpa probiotik, prebiotik, dan sinbiotik kemudian diuji tantang dengan WSSV), kontrol negatif (pemberian pakan tanpa probiotik, prebiotik, dan sinbiotik kemudian diinjeksi PBS), pro (pemberian pakan dengan penambahan probiotik Bacillus sp. NP5 kemudian diuji tantang dengan WSSV), pre (pemberian pakan dengan penambahan prebiotik madu kemudian diuji tantang WSSV), dan sin (pemberian pakan dengan penambahan sinbiotik kemudian diuji tantang dengan WSSV). Udang vaname dengan bobot rata-rata 1.8±0.06 gram/ekor dipelihara dengan kepadatan 15 ekor per akuarium (60 cm x 30 cm x 30 cm) selama delapan minggu, kemudian diuji tantang dengan WSSV sebanyak 0.1 ml per ekor pada dosis LD50. Hasil penelitian menunjukkan bahwa respons imun dan resistansi udang vaname setelah pemberian probiotik, prebiotik dan sinbiotik lebih baik (P<0.05) dibandingkan kontrol dengan hasil optimal pada perlakuan prebiotik.
Kata kunci: Bacillus sp. NP5, madu, respons imun, udang vaname, WSSV.
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| format | Article |
| id | doaj-art-ef0b04ed207c4ef9a9500a5d3d8296b2 |
| institution | Kabale University |
| issn | 1412-5269 2354-6700 |
| language | English |
| publishDate | 2020-09-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj-art-ef0b04ed207c4ef9a9500a5d3d8296b22025-01-24T17:56:47ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002020-09-0119210.19027/jai.19.2.118-13028498Immune responses and resistance of white shrimp (Litopenaeus vannamei) fed Probiotic Bacillus sp NP5 and prebiotic honey against White Spot Syndrome Virus infectionWidanarni Widanarni0Dewi Rahmi1Muhamad Gustilatov2Sukenda Sukenda3Diah Ayu Satyari Utami4Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB UniversityDepartment of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB UniversityDepartment of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB UniversityDepartment of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB UniversityDepartment of Aquaculture, Marine and Fisheries Polytechnic of Jembrana, Jembrana, Bali, Indonesia ABSTRACT White spot disease caused by White Spot Syndrome Virus (WSSV) is the most serious viral disease and has a major impact on the decline in production of white shrimp farm. Improving the immune response through the application of prebiotic, probiotic and synbiotic is expected to be one of the environmentally friendly alternatives to prevent the disease. This study aimed to evaluate the effect of administrating Baccillus sp. NP5 probiotic, honey prebiotic and a combination both (synbiotic) in enhancing immune response and resistence of white shrimp to WSSV infection. This study consisted of five treatments and three replications, namely positive control (feeding without probiotics, prebiotics, and synbiotics then challenged with WSSV), negative control (feeding without probiotics, prebiotics, synbiotics, then injected with PBS), pro (feeding with the addition of Bacillus sp. NP5 probiotics then challenged against WSSV), pre (feeding with the addition of honey prebiotics then challenged against WSSV), and sin (feeding with the addition of synbiotics then challenged against WSSV). White shrimp with an average weight of 1.8±0.06 gram/shrimp were reared at a density of 15 shrimps per aquarium (60 cm x 30 cm x 30 cm and water height of 20 cm) for eight weeks, then challenge against WSSV at lethal dose (LD50) dose as much as 0.1 ml per shrimp. The results showed that the immune response and resistence of white shrimp after treatments of probiotics, prebiotics, and synbiotics were better than those in controls with the optimal found in prebiotics treatment. Keywords: Bacillus sp. NP5, honey, immune response, white shrimp, WSSV ABSTRAK White spot disease yang disebabkan oleh infeksi white spot syndrome virus (WSSV) merupakan penyakit viral paling serius dan berdampak besar terhadap penurunan produksi budidaya udang vaname. Perbaikan respons imun melalui aplikasi probiotik, prebiotik dan sinbiotik dapat menjadi salah satu alternatif ramah lingkungan untuk pencegahan serangan penyakit tersebut. Penelitian ini bertujuan untuk mengevaluasi pengaruh pemberian probiotik Bacillus sp. NP5, prebiotik madu dan gabungan keduanya (sinbiotik) dalam meningkatkan respons imun dan resistansi udang vaname terhadap infeksi WSSV. Penelitian ini terdiri dari lima perlakuan dan tiga ulangan yaitu kontrol positif (pemberian pakan tanpa probiotik, prebiotik, dan sinbiotik kemudian diuji tantang dengan WSSV), kontrol negatif (pemberian pakan tanpa probiotik, prebiotik, dan sinbiotik kemudian diinjeksi PBS), pro (pemberian pakan dengan penambahan probiotik Bacillus sp. NP5 kemudian diuji tantang dengan WSSV), pre (pemberian pakan dengan penambahan prebiotik madu kemudian diuji tantang WSSV), dan sin (pemberian pakan dengan penambahan sinbiotik kemudian diuji tantang dengan WSSV). Udang vaname dengan bobot rata-rata 1.8±0.06 gram/ekor dipelihara dengan kepadatan 15 ekor per akuarium (60 cm x 30 cm x 30 cm) selama delapan minggu, kemudian diuji tantang dengan WSSV sebanyak 0.1 ml per ekor pada dosis LD50. Hasil penelitian menunjukkan bahwa respons imun dan resistansi udang vaname setelah pemberian probiotik, prebiotik dan sinbiotik lebih baik (P<0.05) dibandingkan kontrol dengan hasil optimal pada perlakuan prebiotik. Kata kunci: Bacillus sp. NP5, madu, respons imun, udang vaname, WSSV. https://journal.ipb.ac.id/index.php/jai/article/view/28498 |
| spellingShingle | Widanarni Widanarni Dewi Rahmi Muhamad Gustilatov Sukenda Sukenda Diah Ayu Satyari Utami Immune responses and resistance of white shrimp (Litopenaeus vannamei) fed Probiotic Bacillus sp NP5 and prebiotic honey against White Spot Syndrome Virus infection Jurnal Akuakultur Indonesia |
| title | Immune responses and resistance of white shrimp (Litopenaeus vannamei) fed Probiotic Bacillus sp NP5 and prebiotic honey against White Spot Syndrome Virus infection |
| title_full | Immune responses and resistance of white shrimp (Litopenaeus vannamei) fed Probiotic Bacillus sp NP5 and prebiotic honey against White Spot Syndrome Virus infection |
| title_fullStr | Immune responses and resistance of white shrimp (Litopenaeus vannamei) fed Probiotic Bacillus sp NP5 and prebiotic honey against White Spot Syndrome Virus infection |
| title_full_unstemmed | Immune responses and resistance of white shrimp (Litopenaeus vannamei) fed Probiotic Bacillus sp NP5 and prebiotic honey against White Spot Syndrome Virus infection |
| title_short | Immune responses and resistance of white shrimp (Litopenaeus vannamei) fed Probiotic Bacillus sp NP5 and prebiotic honey against White Spot Syndrome Virus infection |
| title_sort | immune responses and resistance of white shrimp litopenaeus vannamei fed probiotic bacillus sp np5 and prebiotic honey against white spot syndrome virus infection |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/28498 |
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