Identification of Fasciola Species Isolates from Nghe An Province, Vietnam, Based on ITS1 Sequence of Ribosomal DNA Using a Simple PCR-RFLP Method
Fascioliasis—a disease caused by Fasciola spp. (Platyhelminthes: Trematoda: Digenea)—is considered as the most important helminthic infection of bovine, sheep, and buffalo in Vietnam. The aim of this study is to detect the genotype of Fasciola spp. isolated from bovine and buffalo in the Nghe An pro...
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Wiley
2018-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/2958026 |
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| author | Do Ngoc Anh Le Tran Anh Le Quoc Tuan Nguyen Duy Bac Tran Viet Tien Vu Thi Binh Phuong Tran Thanh Duong Nguyen Khac Luc Nguyen Ba Quang |
| author_facet | Do Ngoc Anh Le Tran Anh Le Quoc Tuan Nguyen Duy Bac Tran Viet Tien Vu Thi Binh Phuong Tran Thanh Duong Nguyen Khac Luc Nguyen Ba Quang |
| author_sort | Do Ngoc Anh |
| collection | DOAJ |
| description | Fascioliasis—a disease caused by Fasciola spp. (Platyhelminthes: Trematoda: Digenea)—is considered as the most important helminthic infection of bovine, sheep, and buffalo in Vietnam. The aim of this study is to detect the genotype of Fasciola spp. isolated from bovine and buffalo in the Nghe An province, central Vietnam, using PCR-RFLP and sequence analysis of the first nuclear ribosomal internal transcribed spacer (ITS1). Adult Fasciola spp. were isolated from bile ducts of bovine and buffalo in Nghe An province, Vietnam. Overall, 96 adult flukes from livers of slaughtered animals were collected from abattoirs of different areas. They included 7 samples from infected bovine and 89 samples from infected buffalo. 96/96 samples were identified as Fasciola species by ITS1 of rDNA. In this study, a PCR-RFLP method was used to distinguish between F. hepatica and F. gigantica in ITS1 of rDNA (680 bp) with RsaI restriction enzyme. RFLP pattern with RsaI produced a consistent pattern of 360, 100, and 60 bp fragments in F. hepatica, whereas F. gigantica worms had a profile of 360, 170, and 60 bp in size, respectively. The results showed that using PCR-RFLP based on the first internal transcribed spacers (ITS1) of the ribosomal RNA revealed that 93 out of 96 isolates were of Fasciola gigantica type, whereas three isolates presented an intermediate Fasciola. In the present study, F. gigantica and intermediate form were coexisting in bovine and buffalo in the Nghe An province of central Vietnam, whereas F. hepatica was not detected. |
| format | Article |
| id | doaj-art-eefdb15b4fcc43e395bbd073ec5c1f6f |
| institution | OA Journals |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
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| series | Journal of Parasitology Research |
| spelling | doaj-art-eefdb15b4fcc43e395bbd073ec5c1f6f2025-08-20T02:22:25ZengWileyJournal of Parasitology Research2090-00232090-00312018-01-01201810.1155/2018/29580262958026Identification of Fasciola Species Isolates from Nghe An Province, Vietnam, Based on ITS1 Sequence of Ribosomal DNA Using a Simple PCR-RFLP MethodDo Ngoc Anh0Le Tran Anh1Le Quoc Tuan2Nguyen Duy Bac3Tran Viet Tien4Vu Thi Binh Phuong5Tran Thanh Duong6Nguyen Khac Luc7Nguyen Ba Quang8Department of Medical Parasitology, Vietnam Military Medical University, Hanoi 100000, VietnamDepartment of Medical Parasitology, Vietnam Military Medical University, Hanoi 100000, VietnamDepartment of Medical Parasitology, Vietnam Military Medical University, Hanoi 100000, VietnamDepartment of Anatomy, Vietnam Military Medical University, Hanoi 100000, VietnamDepartment of Infectious Diseases, Vietnam Military Medical University, Hanoi 100000, VietnamDepartment of Medical Parasitology, Thai Binh University of Medicine and Pharmacy, Thai Binh 410000, VietnamNational Institute of Malariology Parasitology and Entomology Vietnam, Hanoi 100000, VietnamDepartment of Medical Parasitology, Vietnam Military Medical University, Hanoi 100000, VietnamDepartment of Laboratory Medicine, Vietnam National Hospital of Acupuncture, Hanoi 100000, VietnamFascioliasis—a disease caused by Fasciola spp. (Platyhelminthes: Trematoda: Digenea)—is considered as the most important helminthic infection of bovine, sheep, and buffalo in Vietnam. The aim of this study is to detect the genotype of Fasciola spp. isolated from bovine and buffalo in the Nghe An province, central Vietnam, using PCR-RFLP and sequence analysis of the first nuclear ribosomal internal transcribed spacer (ITS1). Adult Fasciola spp. were isolated from bile ducts of bovine and buffalo in Nghe An province, Vietnam. Overall, 96 adult flukes from livers of slaughtered animals were collected from abattoirs of different areas. They included 7 samples from infected bovine and 89 samples from infected buffalo. 96/96 samples were identified as Fasciola species by ITS1 of rDNA. In this study, a PCR-RFLP method was used to distinguish between F. hepatica and F. gigantica in ITS1 of rDNA (680 bp) with RsaI restriction enzyme. RFLP pattern with RsaI produced a consistent pattern of 360, 100, and 60 bp fragments in F. hepatica, whereas F. gigantica worms had a profile of 360, 170, and 60 bp in size, respectively. The results showed that using PCR-RFLP based on the first internal transcribed spacers (ITS1) of the ribosomal RNA revealed that 93 out of 96 isolates were of Fasciola gigantica type, whereas three isolates presented an intermediate Fasciola. In the present study, F. gigantica and intermediate form were coexisting in bovine and buffalo in the Nghe An province of central Vietnam, whereas F. hepatica was not detected.http://dx.doi.org/10.1155/2018/2958026 |
| spellingShingle | Do Ngoc Anh Le Tran Anh Le Quoc Tuan Nguyen Duy Bac Tran Viet Tien Vu Thi Binh Phuong Tran Thanh Duong Nguyen Khac Luc Nguyen Ba Quang Identification of Fasciola Species Isolates from Nghe An Province, Vietnam, Based on ITS1 Sequence of Ribosomal DNA Using a Simple PCR-RFLP Method Journal of Parasitology Research |
| title | Identification of Fasciola Species Isolates from Nghe An Province, Vietnam, Based on ITS1 Sequence of Ribosomal DNA Using a Simple PCR-RFLP Method |
| title_full | Identification of Fasciola Species Isolates from Nghe An Province, Vietnam, Based on ITS1 Sequence of Ribosomal DNA Using a Simple PCR-RFLP Method |
| title_fullStr | Identification of Fasciola Species Isolates from Nghe An Province, Vietnam, Based on ITS1 Sequence of Ribosomal DNA Using a Simple PCR-RFLP Method |
| title_full_unstemmed | Identification of Fasciola Species Isolates from Nghe An Province, Vietnam, Based on ITS1 Sequence of Ribosomal DNA Using a Simple PCR-RFLP Method |
| title_short | Identification of Fasciola Species Isolates from Nghe An Province, Vietnam, Based on ITS1 Sequence of Ribosomal DNA Using a Simple PCR-RFLP Method |
| title_sort | identification of fasciola species isolates from nghe an province vietnam based on its1 sequence of ribosomal dna using a simple pcr rflp method |
| url | http://dx.doi.org/10.1155/2018/2958026 |
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