Bubble cloud-mediated cavitation for tumor mechanical ablation and effector immune cell deployment
Histotripsy is a cavitation-based tumor ablation technology. To achieve precise cavitation-based ablation requires investigating the cavitation behavior of the bubble cloud and their impact on tumor tissue. This study explored the cavitation behavior of bubble clouds generated by perfluoropentane (P...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-04-01
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| Series: | Ultrasonics Sonochemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417725000756 |
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| author | Jing Cao Ling Wang Jiarui Li Mengyu Song Yinuo Zheng Xiangling He Xiaoying Li Songcheng Xu Litao Sun |
| author_facet | Jing Cao Ling Wang Jiarui Li Mengyu Song Yinuo Zheng Xiangling He Xiaoying Li Songcheng Xu Litao Sun |
| author_sort | Jing Cao |
| collection | DOAJ |
| description | Histotripsy is a cavitation-based tumor ablation technology. To achieve precise cavitation-based ablation requires investigating the cavitation behavior of the bubble cloud and their impact on tumor tissue. This study explored the cavitation behavior of bubble clouds generated by perfluoropentane (PFP)-loaded nanodroplets and efficacy of bubble cloud cavitation in tumor ablation under varying ultrasound intensities. PFP-loaded nanodroplets (∼200 nm) were employed as exogenous cavitation nuclei to reducing the required ultrasound energy for activation of bubble cloud. We investigated the formation, vibration, and collapse of bubble clouds in solution and phantom models under varying ultrasound intensities. Results indicated distinct cavitation patterns: (1) Nanodroplets slowly vaporized and formed continuously vibrating bubble clouds; (2) Nanodroplets rapidly vaporized and resulted in quickly collapsing bubble clouds. At both the cellular and animal levels, cavitation ablation efficacy was examined, revealing that all bubble cloud cavitation patterns could induce immunogenic cell death (ICD), promoting the release of damage-associated molecular patterns (DAMPs) and triggering effector immune cell deployment of peripheral immune response and local tumor infiltration. During the treatment, the ultrasound intensity of 0.5 W/cm2 had the highest level of central tumor CD8+ T cell infiltration. The conclusion was that sustained bubble cloud oscillation, rather than rapid vaporization and rupture, proved more beneficial for antitumor therapy, particularly in enhancing the local infiltration of effector immune cells. |
| format | Article |
| id | doaj-art-eed58ed018b94f71b48e3481c15c2d35 |
| institution | DOAJ |
| issn | 1350-4177 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj-art-eed58ed018b94f71b48e3481c15c2d352025-08-20T03:07:24ZengElsevierUltrasonics Sonochemistry1350-41772025-04-0111510729610.1016/j.ultsonch.2025.107296Bubble cloud-mediated cavitation for tumor mechanical ablation and effector immune cell deploymentJing Cao0Ling Wang1Jiarui Li2Mengyu Song3Yinuo Zheng4Xiangling He5Xiaoying Li6Songcheng Xu7Litao Sun8Cancer Center, Department of Ultrasound Medicine, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, ChinaCancer Center, Department of Ultrasound Medicine, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang, ChinaCancer Center, Department of Ultrasound Medicine, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang, ChinaCancer Center, Department of Medical Oncology, Zhejiang Provincial People’s Hospital, Hangzhou Medical College, Hangzhou, Zhejiang, ChinaCancer Center, Department of Ultrasound Medicine, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China; Second School of Clinical Medicine, Hangzhou Normal University, Hangzhou, Zhejiang, ChinaJinzhou Medical University Postgraduate Training Base (Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College), Hangzhou, Zhejiang, ChinaCancer Center, Department of Ultrasound Medicine, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, ChinaCancer Center, Department of Ultrasound Medicine, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, ChinaCancer Center, Department of Ultrasound Medicine, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China; Corresponding author.Histotripsy is a cavitation-based tumor ablation technology. To achieve precise cavitation-based ablation requires investigating the cavitation behavior of the bubble cloud and their impact on tumor tissue. This study explored the cavitation behavior of bubble clouds generated by perfluoropentane (PFP)-loaded nanodroplets and efficacy of bubble cloud cavitation in tumor ablation under varying ultrasound intensities. PFP-loaded nanodroplets (∼200 nm) were employed as exogenous cavitation nuclei to reducing the required ultrasound energy for activation of bubble cloud. We investigated the formation, vibration, and collapse of bubble clouds in solution and phantom models under varying ultrasound intensities. Results indicated distinct cavitation patterns: (1) Nanodroplets slowly vaporized and formed continuously vibrating bubble clouds; (2) Nanodroplets rapidly vaporized and resulted in quickly collapsing bubble clouds. At both the cellular and animal levels, cavitation ablation efficacy was examined, revealing that all bubble cloud cavitation patterns could induce immunogenic cell death (ICD), promoting the release of damage-associated molecular patterns (DAMPs) and triggering effector immune cell deployment of peripheral immune response and local tumor infiltration. During the treatment, the ultrasound intensity of 0.5 W/cm2 had the highest level of central tumor CD8+ T cell infiltration. The conclusion was that sustained bubble cloud oscillation, rather than rapid vaporization and rupture, proved more beneficial for antitumor therapy, particularly in enhancing the local infiltration of effector immune cells.http://www.sciencedirect.com/science/article/pii/S1350417725000756Acoustic droplet vaporizationBubble cloudUltrasound cavitationPerfluoropentaneTumor treatment |
| spellingShingle | Jing Cao Ling Wang Jiarui Li Mengyu Song Yinuo Zheng Xiangling He Xiaoying Li Songcheng Xu Litao Sun Bubble cloud-mediated cavitation for tumor mechanical ablation and effector immune cell deployment Ultrasonics Sonochemistry Acoustic droplet vaporization Bubble cloud Ultrasound cavitation Perfluoropentane Tumor treatment |
| title | Bubble cloud-mediated cavitation for tumor mechanical ablation and effector immune cell deployment |
| title_full | Bubble cloud-mediated cavitation for tumor mechanical ablation and effector immune cell deployment |
| title_fullStr | Bubble cloud-mediated cavitation for tumor mechanical ablation and effector immune cell deployment |
| title_full_unstemmed | Bubble cloud-mediated cavitation for tumor mechanical ablation and effector immune cell deployment |
| title_short | Bubble cloud-mediated cavitation for tumor mechanical ablation and effector immune cell deployment |
| title_sort | bubble cloud mediated cavitation for tumor mechanical ablation and effector immune cell deployment |
| topic | Acoustic droplet vaporization Bubble cloud Ultrasound cavitation Perfluoropentane Tumor treatment |
| url | http://www.sciencedirect.com/science/article/pii/S1350417725000756 |
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