Proteomic Profiling and Functional Analysis of B Cell-Derived Exosomes upon Pneumocystis Infection
Pneumocystis is a life-threatening fungal pathogen that frequently causes fatal pneumonia (PCP) in immunocompromised individuals. Recently, B cells have been reported to play a crucial role in the pathogenesis of PCP through producing antibodies and activating CD4+ T cell response. Exosomes are nano...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/5187166 |
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| author | Dan Ma Qian-Yu Zhang Heng-Mo Rong Kan Zhai Zhao-Hui Tong |
| author_facet | Dan Ma Qian-Yu Zhang Heng-Mo Rong Kan Zhai Zhao-Hui Tong |
| author_sort | Dan Ma |
| collection | DOAJ |
| description | Pneumocystis is a life-threatening fungal pathogen that frequently causes fatal pneumonia (PCP) in immunocompromised individuals. Recently, B cells have been reported to play a crucial role in the pathogenesis of PCP through producing antibodies and activating CD4+ T cell response. Exosomes are nanoscale small extracellular vesicles abundant with protein cargo and can mediate immune response during infectious disease. In this study, using tandem mass tag-based quantitative proteomics coupled with bioinformatic analysis, we attempted to characterize exosomes derived from B lymphocytes in response to PCP. Several proteins were verified by parallel reaction monitoring (PRM) analysis. Also, the effects of B cell exosomes on CD4+ T cell response and phagocytic function of macrophages were clarified. Briefly, 1701 proteins were identified from B cell exosomes, and the majority of them were reported in Vesiclepedia. A total of 51 differentially expressed proteins of B cell exosomes were found in response to PCP. They were mainly associated with immune response and transcription regulation. PRM analysis confirmed the significantly changed levels of histone H1.3, vimentin, and tyrosine-protein phosphatase nonreceptor type 6 (PTPN6). Moreover, a functional study revealed the proinflammatory profile of B cell exosomes on CD4+ T cell response in PCP. Taken together, our results suggest the involvement of exosomes derived from B cells in cell-to-cell communication, providing new information on the function of B cells in response to PCP. |
| format | Article |
| id | doaj-art-eec58a7aab0747189760461200c2bddf |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-eec58a7aab0747189760461200c2bddf2025-02-03T01:06:33ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/5187166Proteomic Profiling and Functional Analysis of B Cell-Derived Exosomes upon Pneumocystis InfectionDan Ma0Qian-Yu Zhang1Heng-Mo Rong2Kan Zhai3Zhao-Hui Tong4Department of Respiratory and Critical Care MedicineDepartment of Respiratory and Critical Care MedicineDepartment of Respiratory and Critical Care MedicineDepartment of Respiratory and Critical Care MedicineDepartment of Respiratory and Critical Care MedicinePneumocystis is a life-threatening fungal pathogen that frequently causes fatal pneumonia (PCP) in immunocompromised individuals. Recently, B cells have been reported to play a crucial role in the pathogenesis of PCP through producing antibodies and activating CD4+ T cell response. Exosomes are nanoscale small extracellular vesicles abundant with protein cargo and can mediate immune response during infectious disease. In this study, using tandem mass tag-based quantitative proteomics coupled with bioinformatic analysis, we attempted to characterize exosomes derived from B lymphocytes in response to PCP. Several proteins were verified by parallel reaction monitoring (PRM) analysis. Also, the effects of B cell exosomes on CD4+ T cell response and phagocytic function of macrophages were clarified. Briefly, 1701 proteins were identified from B cell exosomes, and the majority of them were reported in Vesiclepedia. A total of 51 differentially expressed proteins of B cell exosomes were found in response to PCP. They were mainly associated with immune response and transcription regulation. PRM analysis confirmed the significantly changed levels of histone H1.3, vimentin, and tyrosine-protein phosphatase nonreceptor type 6 (PTPN6). Moreover, a functional study revealed the proinflammatory profile of B cell exosomes on CD4+ T cell response in PCP. Taken together, our results suggest the involvement of exosomes derived from B cells in cell-to-cell communication, providing new information on the function of B cells in response to PCP.http://dx.doi.org/10.1155/2022/5187166 |
| spellingShingle | Dan Ma Qian-Yu Zhang Heng-Mo Rong Kan Zhai Zhao-Hui Tong Proteomic Profiling and Functional Analysis of B Cell-Derived Exosomes upon Pneumocystis Infection Journal of Immunology Research |
| title | Proteomic Profiling and Functional Analysis of B Cell-Derived Exosomes upon Pneumocystis Infection |
| title_full | Proteomic Profiling and Functional Analysis of B Cell-Derived Exosomes upon Pneumocystis Infection |
| title_fullStr | Proteomic Profiling and Functional Analysis of B Cell-Derived Exosomes upon Pneumocystis Infection |
| title_full_unstemmed | Proteomic Profiling and Functional Analysis of B Cell-Derived Exosomes upon Pneumocystis Infection |
| title_short | Proteomic Profiling and Functional Analysis of B Cell-Derived Exosomes upon Pneumocystis Infection |
| title_sort | proteomic profiling and functional analysis of b cell derived exosomes upon pneumocystis infection |
| url | http://dx.doi.org/10.1155/2022/5187166 |
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