Hepatic Cerebroside Sulfotransferase Is Induced by PPARα Activation in Mice
Sulfatides are one of the major sphingoglycolipids in mammalian serum and are synthesized and secreted mainly from the liver as a component of lipoproteins. Recent studies revealed a protective role for serum sulfatides against arteriosclerosis and hypercoagulation. Although peroxisome proliferator-...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2012/174932 |
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| author | Takefumi Kimura Takero Nakajima Yuji Kamijo Naoki Tanaka Lixuan Wang Atsushi Hara Eiko Sugiyama Eiji Tanaka Frank J. Gonzalez Toshifumi Aoyama |
| author_facet | Takefumi Kimura Takero Nakajima Yuji Kamijo Naoki Tanaka Lixuan Wang Atsushi Hara Eiko Sugiyama Eiji Tanaka Frank J. Gonzalez Toshifumi Aoyama |
| author_sort | Takefumi Kimura |
| collection | DOAJ |
| description | Sulfatides are one of the major sphingoglycolipids in mammalian serum and are synthesized and secreted mainly from the liver as a component of lipoproteins. Recent studies revealed a protective role for serum sulfatides against arteriosclerosis and hypercoagulation. Although peroxisome proliferator-activated receptor (PPAR) α has important functions in hepatic lipoprotein metabolism, its association with sulfatides has not been investigated. In this study, sulfatide levels and the expression of enzymes related to sulfatide metabolism were examined using wild-type (+/+), Ppara-heterozygous (+/−), and Ppara-null (−/−) mice given a control diet or one containing 0.1% fenofibrate, a clinically used hypolipidemic drug and PPARα activator. Fenofibrate treatment increased serum and hepatic sulfatides in Ppara (+/+) and (+/−) mice through a marked induction of hepatic cerebroside sulfotransferase (CST), a key enzyme in sulfatide synthesis, in a PPARα-dependent manner. Furthermore, increases in CST mRNA levels were correlated with mRNA elevations of several known PPARα target genes, and such changes were not observed for other sulfatide-metabolism enzymes in the liver. These results suggest that PPARα activation enhances hepatic sulfatide synthesis via CST induction and implicate CST as a novel PPARα target gene. |
| format | Article |
| id | doaj-art-eeb6d13042e54a7ea9fb749c3d6a166e |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-eeb6d13042e54a7ea9fb749c3d6a166e2025-02-03T06:11:45ZengWileyPPAR Research1687-47571687-47652012-01-01201210.1155/2012/174932174932Hepatic Cerebroside Sulfotransferase Is Induced by PPARα Activation in MiceTakefumi Kimura0Takero Nakajima1Yuji Kamijo2Naoki Tanaka3Lixuan Wang4Atsushi Hara5Eiko Sugiyama6Eiji Tanaka7Frank J. Gonzalez8Toshifumi Aoyama9Department of Metabolic Regulation, Institute on Aging and Adaptation, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, JapanDepartment of Metabolic Regulation, Institute on Aging and Adaptation, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, JapanDepartment of Metabolic Regulation, Institute on Aging and Adaptation, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, JapanDepartment of Metabolic Regulation, Institute on Aging and Adaptation, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, JapanDepartment of Metabolic Regulation, Institute on Aging and Adaptation, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, JapanDepartment of Metabolic Regulation, Institute on Aging and Adaptation, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, JapanDepartment of Nutritional Science, Nagano Prefectural College, Nagano 380-8525, JapanDepartment of Gastroenterology, Shinshu University School of Medicine, Matsumoto 390-8621, JapanLaboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USADepartment of Metabolic Regulation, Institute on Aging and Adaptation, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, JapanSulfatides are one of the major sphingoglycolipids in mammalian serum and are synthesized and secreted mainly from the liver as a component of lipoproteins. Recent studies revealed a protective role for serum sulfatides against arteriosclerosis and hypercoagulation. Although peroxisome proliferator-activated receptor (PPAR) α has important functions in hepatic lipoprotein metabolism, its association with sulfatides has not been investigated. In this study, sulfatide levels and the expression of enzymes related to sulfatide metabolism were examined using wild-type (+/+), Ppara-heterozygous (+/−), and Ppara-null (−/−) mice given a control diet or one containing 0.1% fenofibrate, a clinically used hypolipidemic drug and PPARα activator. Fenofibrate treatment increased serum and hepatic sulfatides in Ppara (+/+) and (+/−) mice through a marked induction of hepatic cerebroside sulfotransferase (CST), a key enzyme in sulfatide synthesis, in a PPARα-dependent manner. Furthermore, increases in CST mRNA levels were correlated with mRNA elevations of several known PPARα target genes, and such changes were not observed for other sulfatide-metabolism enzymes in the liver. These results suggest that PPARα activation enhances hepatic sulfatide synthesis via CST induction and implicate CST as a novel PPARα target gene.http://dx.doi.org/10.1155/2012/174932 |
| spellingShingle | Takefumi Kimura Takero Nakajima Yuji Kamijo Naoki Tanaka Lixuan Wang Atsushi Hara Eiko Sugiyama Eiji Tanaka Frank J. Gonzalez Toshifumi Aoyama Hepatic Cerebroside Sulfotransferase Is Induced by PPARα Activation in Mice PPAR Research |
| title | Hepatic Cerebroside Sulfotransferase Is Induced by PPARα Activation in Mice |
| title_full | Hepatic Cerebroside Sulfotransferase Is Induced by PPARα Activation in Mice |
| title_fullStr | Hepatic Cerebroside Sulfotransferase Is Induced by PPARα Activation in Mice |
| title_full_unstemmed | Hepatic Cerebroside Sulfotransferase Is Induced by PPARα Activation in Mice |
| title_short | Hepatic Cerebroside Sulfotransferase Is Induced by PPARα Activation in Mice |
| title_sort | hepatic cerebroside sulfotransferase is induced by pparα activation in mice |
| url | http://dx.doi.org/10.1155/2012/174932 |
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