Analysis of Circulating MicroRNAs In Vivo following Administration of Dexamethasone and Adrenocorticotropin
Purpose. The interaction of hormones of the pituitary-adrenal axis and adrenal cortex-associated circulating microRNAs is mostly unknown. We have studied the effects of dexamethasone and adrenocorticotropin on the expression of five circulating microRNAs (hsa-miR-27a, hsa-miR-200b, hsa-miR-214, hsa-...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2015/589230 |
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| author | Ivan Igaz Gábor Nyírő Zoltán Nagy Henriett Butz Zsolt Nagy Pál Perge Peter Sahin Miklós Tóth Károly Rácz Peter Igaz Attila Patócs |
| author_facet | Ivan Igaz Gábor Nyírő Zoltán Nagy Henriett Butz Zsolt Nagy Pál Perge Peter Sahin Miklós Tóth Károly Rácz Peter Igaz Attila Patócs |
| author_sort | Ivan Igaz |
| collection | DOAJ |
| description | Purpose. The interaction of hormones of the pituitary-adrenal axis and adrenal cortex-associated circulating microRNAs is mostly unknown. We have studied the effects of dexamethasone and adrenocorticotropin on the expression of five circulating microRNAs (hsa-miR-27a, hsa-miR-200b, hsa-miR-214, hsa-miR-483-5p, and hsa-miR-503) reported to be related to the adrenal cortex in plasma samples. Methods. Expression of microRNAs was studied in plasma samples of 10 individuals examined by 1 mg dexamethasone suppression test and another 10 individuals stimulated by 250 μg tetracosactide (adrenocorticotropin). Total RNA was isolated and microRNA expression was analyzed by real-time reverse transcription quantitative polymerase chain reaction normalized to cel-miR-39 as reference. Results. Only circulating hsa-miR-27a proved to be significantly modulated in vivo by hormonal treatments: its expression was upregulated by dexamethasone whereas it was suppressed by adrenocorticotropin. Secreted hsa-miR-27a was significantly induced by dexamethasone in vitro in NCI-H295R cells, as well. The expression of hsa-miR-483-5p proposed as diagnostic marker for adrenocortical malignancy was not affected by dexamethasone or tetracosactide administration. Conclusions. hsa-miR-27a expression is modulated by hormones of the hypothalamic-pituitary-adrenal axis both in vitro and in vivo. The biological relevance of hsa-miR-27a modulation by hormones is unclear, but the responsiveness of circulating microRNAs to hormones of the pituitary-adrenal axis is noteworthy. |
| format | Article |
| id | doaj-art-ee79aa9e59c04a069f61dd40515a6340 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-ee79aa9e59c04a069f61dd40515a63402025-02-03T01:12:27ZengWileyInternational Journal of Endocrinology1687-83371687-83452015-01-01201510.1155/2015/589230589230Analysis of Circulating MicroRNAs In Vivo following Administration of Dexamethasone and AdrenocorticotropinIvan Igaz0Gábor Nyírő1Zoltán Nagy2Henriett Butz3Zsolt Nagy4Pál Perge5Peter Sahin6Miklós Tóth7Károly Rácz8Peter Igaz9Attila Patócs10Department of Gastroenterology, Szent Imre Teaching Hospital, Tétényi Street 12-16, Budapest 1115, HungaryMolecular Medicine Research Group, Hungarian Academy of Sciences and Semmelweis University, Szentkirályi Street 46, Budapest 1088, Hungary2nd Department of Medicine, Faculty of Medicine, Semmelweis University, Szentkirályi Street 46, Budapest 1088, HungaryMolecular Medicine Research Group, Hungarian Academy of Sciences and Semmelweis University, Szentkirályi Street 46, Budapest 1088, Hungary2nd Department of Medicine, Faculty of Medicine, Semmelweis University, Szentkirályi Street 46, Budapest 1088, Hungary2nd Department of Medicine, Faculty of Medicine, Semmelweis University, Szentkirályi Street 46, Budapest 1088, HungaryDepartment of Gastroenterology, Szent Imre Teaching Hospital, Tétényi Street 12-16, Budapest 1115, Hungary2nd Department of Medicine, Faculty of Medicine, Semmelweis University, Szentkirályi Street 46, Budapest 1088, HungaryMolecular Medicine Research Group, Hungarian Academy of Sciences and Semmelweis University, Szentkirályi Street 46, Budapest 1088, Hungary2nd Department of Medicine, Faculty of Medicine, Semmelweis University, Szentkirályi Street 46, Budapest 1088, Hungary“Lendület-2013” Hereditary Endocrine Tumors Research Group, Hungarian Academy of Sciences and Semmelweis University, Szentkirályi Street 46, Budapest 1088, HungaryPurpose. The interaction of hormones of the pituitary-adrenal axis and adrenal cortex-associated circulating microRNAs is mostly unknown. We have studied the effects of dexamethasone and adrenocorticotropin on the expression of five circulating microRNAs (hsa-miR-27a, hsa-miR-200b, hsa-miR-214, hsa-miR-483-5p, and hsa-miR-503) reported to be related to the adrenal cortex in plasma samples. Methods. Expression of microRNAs was studied in plasma samples of 10 individuals examined by 1 mg dexamethasone suppression test and another 10 individuals stimulated by 250 μg tetracosactide (adrenocorticotropin). Total RNA was isolated and microRNA expression was analyzed by real-time reverse transcription quantitative polymerase chain reaction normalized to cel-miR-39 as reference. Results. Only circulating hsa-miR-27a proved to be significantly modulated in vivo by hormonal treatments: its expression was upregulated by dexamethasone whereas it was suppressed by adrenocorticotropin. Secreted hsa-miR-27a was significantly induced by dexamethasone in vitro in NCI-H295R cells, as well. The expression of hsa-miR-483-5p proposed as diagnostic marker for adrenocortical malignancy was not affected by dexamethasone or tetracosactide administration. Conclusions. hsa-miR-27a expression is modulated by hormones of the hypothalamic-pituitary-adrenal axis both in vitro and in vivo. The biological relevance of hsa-miR-27a modulation by hormones is unclear, but the responsiveness of circulating microRNAs to hormones of the pituitary-adrenal axis is noteworthy.http://dx.doi.org/10.1155/2015/589230 |
| spellingShingle | Ivan Igaz Gábor Nyírő Zoltán Nagy Henriett Butz Zsolt Nagy Pál Perge Peter Sahin Miklós Tóth Károly Rácz Peter Igaz Attila Patócs Analysis of Circulating MicroRNAs In Vivo following Administration of Dexamethasone and Adrenocorticotropin International Journal of Endocrinology |
| title | Analysis of Circulating MicroRNAs In Vivo following Administration of Dexamethasone and Adrenocorticotropin |
| title_full | Analysis of Circulating MicroRNAs In Vivo following Administration of Dexamethasone and Adrenocorticotropin |
| title_fullStr | Analysis of Circulating MicroRNAs In Vivo following Administration of Dexamethasone and Adrenocorticotropin |
| title_full_unstemmed | Analysis of Circulating MicroRNAs In Vivo following Administration of Dexamethasone and Adrenocorticotropin |
| title_short | Analysis of Circulating MicroRNAs In Vivo following Administration of Dexamethasone and Adrenocorticotropin |
| title_sort | analysis of circulating micrornas in vivo following administration of dexamethasone and adrenocorticotropin |
| url | http://dx.doi.org/10.1155/2015/589230 |
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