Pectinolytic and Cellulolytic Enzymes Enhance Fusarium compactum Virulence on Tubercles Infection of Egyptian Broomrape
The use of enzyme could facilitate pathogen penetration into plant host. Here the combination of cellulase and pectinase was ascertained on the pathogenicity of F. compactum (1.4×106 propagules ml−1) on broomrape tubercles. F. compactum alone infected all the inoculated tubercles but did not kill...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2010/273264 |
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| author | Olubukola O. Babalola |
| author_facet | Olubukola O. Babalola |
| author_sort | Olubukola O. Babalola |
| collection | DOAJ |
| description | The use of enzyme could facilitate pathogen penetration into plant host. Here the combination of cellulase and pectinase was ascertained on the pathogenicity of F. compactum (1.4×106 propagules ml−1) on broomrape tubercles. F. compactum alone infected all the inoculated tubercles but did not kill any significant number. Infested tomato roots that were inoculated with mycelia plus pectinase (20 U ml−1) had over 50% tubercles dead one week after treatment. Those inoculated with mycelia plus cellulase (20 U ml−1) had above 60% mortality. Mixtures of mycelial plus the two enzymes (10 U ml−1 of each enzyme) showed synergy. The activity catalyzed by an enzyme is a measure of the amount of enzyme present. It was shown that, in a 1 mg (10 U mg−1) cellulase used, 0.055 mg pectinase (1.1 U mg−1) is present. This explains why mycelial plus cellulase mix contends with mycelial plus the two enzymes. |
| format | Article |
| id | doaj-art-ee7283ecf0e44906806a48ec9b155ae2 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-ee7283ecf0e44906806a48ec9b155ae22025-02-03T01:01:26ZengWileyInternational Journal of Microbiology1687-918X1687-91982010-01-01201010.1155/2010/273264273264Pectinolytic and Cellulolytic Enzymes Enhance Fusarium compactum Virulence on Tubercles Infection of Egyptian BroomrapeOlubukola O. Babalola0Department of Biological Sciences, Faculty of Agriculture, Science and Technology, North-West University, Mafikeng Campus, Private Bag X2046, Mmabatho 2735, South AfricaThe use of enzyme could facilitate pathogen penetration into plant host. Here the combination of cellulase and pectinase was ascertained on the pathogenicity of F. compactum (1.4×106 propagules ml−1) on broomrape tubercles. F. compactum alone infected all the inoculated tubercles but did not kill any significant number. Infested tomato roots that were inoculated with mycelia plus pectinase (20 U ml−1) had over 50% tubercles dead one week after treatment. Those inoculated with mycelia plus cellulase (20 U ml−1) had above 60% mortality. Mixtures of mycelial plus the two enzymes (10 U ml−1 of each enzyme) showed synergy. The activity catalyzed by an enzyme is a measure of the amount of enzyme present. It was shown that, in a 1 mg (10 U mg−1) cellulase used, 0.055 mg pectinase (1.1 U mg−1) is present. This explains why mycelial plus cellulase mix contends with mycelial plus the two enzymes.http://dx.doi.org/10.1155/2010/273264 |
| spellingShingle | Olubukola O. Babalola Pectinolytic and Cellulolytic Enzymes Enhance Fusarium compactum Virulence on Tubercles Infection of Egyptian Broomrape International Journal of Microbiology |
| title | Pectinolytic and Cellulolytic Enzymes Enhance Fusarium compactum Virulence on Tubercles Infection of Egyptian Broomrape |
| title_full | Pectinolytic and Cellulolytic Enzymes Enhance Fusarium compactum Virulence on Tubercles Infection of Egyptian Broomrape |
| title_fullStr | Pectinolytic and Cellulolytic Enzymes Enhance Fusarium compactum Virulence on Tubercles Infection of Egyptian Broomrape |
| title_full_unstemmed | Pectinolytic and Cellulolytic Enzymes Enhance Fusarium compactum Virulence on Tubercles Infection of Egyptian Broomrape |
| title_short | Pectinolytic and Cellulolytic Enzymes Enhance Fusarium compactum Virulence on Tubercles Infection of Egyptian Broomrape |
| title_sort | pectinolytic and cellulolytic enzymes enhance fusarium compactum virulence on tubercles infection of egyptian broomrape |
| url | http://dx.doi.org/10.1155/2010/273264 |
| work_keys_str_mv | AT olubukolaobabalola pectinolyticandcellulolyticenzymesenhancefusariumcompactumvirulenceontuberclesinfectionofegyptianbroomrape |