Morphology of Peeled Internal Limiting Membrane in Macular Hole Surgery
Purpose. The aim of this work was to describe the ultrastructure and behavior of peeled internal limiting membrane (ILM) in macular hole (MH) surgery. Methods. Seven patients with MH were included, and vitrectomy with ILM peeling was performed in all patients. The ILM inverted flap technique was use...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2019/1345683 |
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| author | Mun Y. Faria David C. Sousa Bruna C. Almeida Andreia L. Pinto Nuno P. Ferreira |
| author_facet | Mun Y. Faria David C. Sousa Bruna C. Almeida Andreia L. Pinto Nuno P. Ferreira |
| author_sort | Mun Y. Faria |
| collection | DOAJ |
| description | Purpose. The aim of this work was to describe the ultrastructure and behavior of peeled internal limiting membrane (ILM) in macular hole (MH) surgery. Methods. Seven patients with MH were included, and vitrectomy with ILM peeling was performed in all patients. The ILM inverted flap technique was used. Two other flaps of ILM of the same patient were collected and studied using light and transmission electron microscopy (TEM). ILM cell type, distribution, and morphology were analyzed, and the proliferation or fusion potential of the ILM interface was evaluated. Results. ILM vitreous sides in apposition showed signs of proliferative fibrotic activity, producing a basal membrane that merges ILM sides. Conclusions. Epiretinal cells in ILM show proliferative capacity, with formation of microfibrils between adjacent sides of the ILM, which may explain adherence of ILM flaps to the hole border, contributing to closure of the hole in MH surgery. This trail is registered with NCT03799575. |
| format | Article |
| id | doaj-art-ee5fba0a5b054070a8b11dcb77b8010a |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-ee5fba0a5b054070a8b11dcb77b8010a2025-02-03T00:59:05ZengWileyJournal of Ophthalmology2090-004X2090-00582019-01-01201910.1155/2019/13456831345683Morphology of Peeled Internal Limiting Membrane in Macular Hole SurgeryMun Y. Faria0David C. Sousa1Bruna C. Almeida2Andreia L. Pinto3Nuno P. Ferreira4Ophthalmology University Clinic, Faculdade de Medicina Lisboa, Universidade de Lisboa, 1649-028 Lisboa, PortugalOphthalmology University Clinic, Faculdade de Medicina Lisboa, Universidade de Lisboa, 1649-028 Lisboa, PortugalHistology and Comparative Pathology Laboratory, Instituto de Medicina Molecular, Lisboa, PortugalHistology and Comparative Pathology Laboratory, Instituto de Medicina Molecular, Lisboa, PortugalOphthalmology University Clinic, Faculdade de Medicina Lisboa, Universidade de Lisboa, 1649-028 Lisboa, PortugalPurpose. The aim of this work was to describe the ultrastructure and behavior of peeled internal limiting membrane (ILM) in macular hole (MH) surgery. Methods. Seven patients with MH were included, and vitrectomy with ILM peeling was performed in all patients. The ILM inverted flap technique was used. Two other flaps of ILM of the same patient were collected and studied using light and transmission electron microscopy (TEM). ILM cell type, distribution, and morphology were analyzed, and the proliferation or fusion potential of the ILM interface was evaluated. Results. ILM vitreous sides in apposition showed signs of proliferative fibrotic activity, producing a basal membrane that merges ILM sides. Conclusions. Epiretinal cells in ILM show proliferative capacity, with formation of microfibrils between adjacent sides of the ILM, which may explain adherence of ILM flaps to the hole border, contributing to closure of the hole in MH surgery. This trail is registered with NCT03799575.http://dx.doi.org/10.1155/2019/1345683 |
| spellingShingle | Mun Y. Faria David C. Sousa Bruna C. Almeida Andreia L. Pinto Nuno P. Ferreira Morphology of Peeled Internal Limiting Membrane in Macular Hole Surgery Journal of Ophthalmology |
| title | Morphology of Peeled Internal Limiting Membrane in Macular Hole Surgery |
| title_full | Morphology of Peeled Internal Limiting Membrane in Macular Hole Surgery |
| title_fullStr | Morphology of Peeled Internal Limiting Membrane in Macular Hole Surgery |
| title_full_unstemmed | Morphology of Peeled Internal Limiting Membrane in Macular Hole Surgery |
| title_short | Morphology of Peeled Internal Limiting Membrane in Macular Hole Surgery |
| title_sort | morphology of peeled internal limiting membrane in macular hole surgery |
| url | http://dx.doi.org/10.1155/2019/1345683 |
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