Multiple action sites of ultrasound on Escherichia coli and Staphylococcus aureus
Ultrasound, is thought to a potential non-thermal sterilization technology in food industry. However, the exact mechanisms underlying microbial inactivation by ultrasound still remain obscure. In this study, the action modes of ultrasound on both Gram-negative and Gram-positive microorganisms were e...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2018-03-01
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| Series: | Food Science and Human Wellness |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345301730191X |
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| author | Xinyu Liao Jiao Li Yuanjie Suo Shiguo Chen Xingqian Ye Donghong Liu Tian Ding |
| author_facet | Xinyu Liao Jiao Li Yuanjie Suo Shiguo Chen Xingqian Ye Donghong Liu Tian Ding |
| author_sort | Xinyu Liao |
| collection | DOAJ |
| description | Ultrasound, is thought to a potential non-thermal sterilization technology in food industry. However, the exact mechanisms underlying microbial inactivation by ultrasound still remain obscure. In this study, the action modes of ultrasound on both Gram-negative and Gram-positive microorganisms were estimated. From colony results, ultrasound acted as an irreversible effect on both Eshcerichia coli and Staphylococcus aureus without sublethal injury. The result in this study also showed that a proportion of bacteria subpopulation suffered from serious damage of intracellular components (e.g. DNA and enzymes) but with intact cell envelopes. We speculated that the inactivated effects of ultrasound on microbes might more than simply completed disruption of cell exteriors. Those microbial cells who had not enter the valid area of ultrasonic cavitation might be injected with free radicals produced by ultrasound and experienced interior injury with intact exterior structure, and others who were in close proximity to the ultrasonic wave field would be immediately and completely disrupted into debris by high power mechanic forces. These findings here try to provide extension for the inactivation mechanisms of ultrasound on microorganisms. Keywords: Ultrasound, Inactivation mechanism, Reactive oxygen species (ROS), Staphylococcus aureus, Escherichia coli |
| format | Article |
| id | doaj-art-ee0a766a6f234751bac0a02a32399a27 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2018-03-01 |
| publisher | Tsinghua University Press |
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| series | Food Science and Human Wellness |
| spelling | doaj-art-ee0a766a6f234751bac0a02a32399a272025-02-03T10:32:34ZengTsinghua University PressFood Science and Human Wellness2213-45302018-03-0171102109Multiple action sites of ultrasound on Escherichia coli and Staphylococcus aureusXinyu Liao0Jiao Li1Yuanjie Suo2Shiguo Chen3Xingqian Ye4Donghong Liu5Tian Ding6Department of Food Science and Nutrition, National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou, Zhejiang 310058, China; Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou, Zhejiang 310058, China; Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, ChinaDepartment of Food Science and Nutrition, National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou, Zhejiang 310058, China; Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou, Zhejiang 310058, ChinaDepartment of Food Science and Nutrition, National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou, Zhejiang 310058, China; Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou, Zhejiang 310058, ChinaDepartment of Food Science and Nutrition, National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou, Zhejiang 310058, China; Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou, Zhejiang 310058, ChinaDepartment of Food Science and Nutrition, National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou, Zhejiang 310058, China; Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou, Zhejiang 310058, ChinaDepartment of Food Science and Nutrition, National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou, Zhejiang 310058, China; Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou, Zhejiang 310058, China; Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, ChinaDepartment of Food Science and Nutrition, National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou, Zhejiang 310058, China; Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou, Zhejiang 310058, China; Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China; Corresponding author at: Department of Food Science and Nutrition, Zhejiang Key 14 Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou, Zhejiang 15 310058, China.Ultrasound, is thought to a potential non-thermal sterilization technology in food industry. However, the exact mechanisms underlying microbial inactivation by ultrasound still remain obscure. In this study, the action modes of ultrasound on both Gram-negative and Gram-positive microorganisms were estimated. From colony results, ultrasound acted as an irreversible effect on both Eshcerichia coli and Staphylococcus aureus without sublethal injury. The result in this study also showed that a proportion of bacteria subpopulation suffered from serious damage of intracellular components (e.g. DNA and enzymes) but with intact cell envelopes. We speculated that the inactivated effects of ultrasound on microbes might more than simply completed disruption of cell exteriors. Those microbial cells who had not enter the valid area of ultrasonic cavitation might be injected with free radicals produced by ultrasound and experienced interior injury with intact exterior structure, and others who were in close proximity to the ultrasonic wave field would be immediately and completely disrupted into debris by high power mechanic forces. These findings here try to provide extension for the inactivation mechanisms of ultrasound on microorganisms. Keywords: Ultrasound, Inactivation mechanism, Reactive oxygen species (ROS), Staphylococcus aureus, Escherichia colihttp://www.sciencedirect.com/science/article/pii/S221345301730191X |
| spellingShingle | Xinyu Liao Jiao Li Yuanjie Suo Shiguo Chen Xingqian Ye Donghong Liu Tian Ding Multiple action sites of ultrasound on Escherichia coli and Staphylococcus aureus Food Science and Human Wellness |
| title | Multiple action sites of ultrasound on Escherichia coli and Staphylococcus aureus |
| title_full | Multiple action sites of ultrasound on Escherichia coli and Staphylococcus aureus |
| title_fullStr | Multiple action sites of ultrasound on Escherichia coli and Staphylococcus aureus |
| title_full_unstemmed | Multiple action sites of ultrasound on Escherichia coli and Staphylococcus aureus |
| title_short | Multiple action sites of ultrasound on Escherichia coli and Staphylococcus aureus |
| title_sort | multiple action sites of ultrasound on escherichia coli and staphylococcus aureus |
| url | http://www.sciencedirect.com/science/article/pii/S221345301730191X |
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