Modern issues of sugar beet (<i>Beta vulgaris</i> L.) hybrid breeding
High efficiency of the cultivation of unfertilized sugar beet ovules and preparation of haploid regenerants (microclones) of pollinators – maintainers of О-type sterility and MS forms of the RMS 120 hybrid components has been shown. A technological method that accelerates the creation of new uniform...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2021-07-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/3040 |
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| author | S. D. Karakotov I. V. Apasov A. A. Nalbandyan E. N. Vasilchenko T. P. Fedulova |
| author_facet | S. D. Karakotov I. V. Apasov A. A. Nalbandyan E. N. Vasilchenko T. P. Fedulova |
| author_sort | S. D. Karakotov |
| collection | DOAJ |
| description | High efficiency of the cultivation of unfertilized sugar beet ovules and preparation of haploid regenerants (microclones) of pollinators – maintainers of О-type sterility and MS forms of the RMS 120 hybrid components has been shown. A technological method that accelerates the creation of new uniform starting material is proposed. It speeds up the breeding process two to threefold. The identification of haploid regenerants with sterile cytoplasm in initial populations is of great theoretical and practical importance for breeding, as it facilitates the production of homozygous lines with cytoplasmic male sterility and high-performance hybrids on sterile basis. As shown by molecular analysis, a single-nucleotide polymorphism never reported hitherto is present in the mitochondrial genome of the haploid plant regenerants. It allows identification of microclones as fertile and sterile forms. It has been found that DNA markers of the sugar beet mitochondrial genome belonging to the TR minisatellite family (TR1 and TR3) enable reliable enough identification of haploid microclonal plants as MSor O-type forms. Fragments of 1000 bp in length have been detected in monogenic forms in the analysis of 11 sugar beet plants cultured in vitro by PCR with the OP-S4 random RAPD primer. Testing of the OP-S4 marker’s being in the same linkage group as the genes responsible for expression of the economically valuable trait monogermity demonstrates its relative reliability. By the proposed method, dihaploid lines (DH) of the male-sterile form and the О-type sterility maintainer of the RMS 120 sugar beet hybrid have been obtained in in vitro culture. These lines are highly uniform in biomorphological traits, as proven under field conditions. |
| format | Article |
| id | doaj-art-ee05ab2c509c42f2b15778c6a992d07b |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2021-07-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-ee05ab2c509c42f2b15778c6a992d07b2025-02-01T09:58:10ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592021-07-0125439440010.18699/VJ21.0431171Modern issues of sugar beet (<i>Beta vulgaris</i> L.) hybrid breedingS. D. Karakotov0I. V. Apasov1A. A. Nalbandyan2E. N. Vasilchenko3T. P. Fedulova4Shchelkovo Agrokhim CompanyThe A.L. Mazlumov All-Russian Research Institute of Sugar Beet and SugarThe A.L. Mazlumov All-Russian Research Institute of Sugar Beet and SugarThe A.L. Mazlumov All-Russian Research Institute of Sugar Beet and SugarThe A.L. Mazlumov All-Russian Research Institute of Sugar Beet and SugarHigh efficiency of the cultivation of unfertilized sugar beet ovules and preparation of haploid regenerants (microclones) of pollinators – maintainers of О-type sterility and MS forms of the RMS 120 hybrid components has been shown. A technological method that accelerates the creation of new uniform starting material is proposed. It speeds up the breeding process two to threefold. The identification of haploid regenerants with sterile cytoplasm in initial populations is of great theoretical and practical importance for breeding, as it facilitates the production of homozygous lines with cytoplasmic male sterility and high-performance hybrids on sterile basis. As shown by molecular analysis, a single-nucleotide polymorphism never reported hitherto is present in the mitochondrial genome of the haploid plant regenerants. It allows identification of microclones as fertile and sterile forms. It has been found that DNA markers of the sugar beet mitochondrial genome belonging to the TR minisatellite family (TR1 and TR3) enable reliable enough identification of haploid microclonal plants as MSor O-type forms. Fragments of 1000 bp in length have been detected in monogenic forms in the analysis of 11 sugar beet plants cultured in vitro by PCR with the OP-S4 random RAPD primer. Testing of the OP-S4 marker’s being in the same linkage group as the genes responsible for expression of the economically valuable trait monogermity demonstrates its relative reliability. By the proposed method, dihaploid lines (DH) of the male-sterile form and the О-type sterility maintainer of the RMS 120 sugar beet hybrid have been obtained in in vitro culture. These lines are highly uniform in biomorphological traits, as proven under field conditions.https://vavilov.elpub.ru/jour/article/view/3040sugar beetmonogermitycytoplasmic male sterilityhomozygous haploid linespcr analysis. |
| spellingShingle | S. D. Karakotov I. V. Apasov A. A. Nalbandyan E. N. Vasilchenko T. P. Fedulova Modern issues of sugar beet (<i>Beta vulgaris</i> L.) hybrid breeding Вавиловский журнал генетики и селекции sugar beet monogermity cytoplasmic male sterility homozygous haploid lines pcr analysis. |
| title | Modern issues of sugar beet (<i>Beta vulgaris</i> L.) hybrid breeding |
| title_full | Modern issues of sugar beet (<i>Beta vulgaris</i> L.) hybrid breeding |
| title_fullStr | Modern issues of sugar beet (<i>Beta vulgaris</i> L.) hybrid breeding |
| title_full_unstemmed | Modern issues of sugar beet (<i>Beta vulgaris</i> L.) hybrid breeding |
| title_short | Modern issues of sugar beet (<i>Beta vulgaris</i> L.) hybrid breeding |
| title_sort | modern issues of sugar beet i beta vulgaris i l hybrid breeding |
| topic | sugar beet monogermity cytoplasmic male sterility homozygous haploid lines pcr analysis. |
| url | https://vavilov.elpub.ru/jour/article/view/3040 |
| work_keys_str_mv | AT sdkarakotov modernissuesofsugarbeetibetavulgarisilhybridbreeding AT ivapasov modernissuesofsugarbeetibetavulgarisilhybridbreeding AT aanalbandyan modernissuesofsugarbeetibetavulgarisilhybridbreeding AT envasilchenko modernissuesofsugarbeetibetavulgarisilhybridbreeding AT tpfedulova modernissuesofsugarbeetibetavulgarisilhybridbreeding |